Term
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Definition
| Moles of solute/kg solvent |
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Term
| Flame emission Spectroscopy |
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Definition
| lithium=red, sodium=yellow, potassium = violet, magnesium =blue. |
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Term
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Definition
Serum tube
1-3 minutes blood will clot
After 3 minutes centrifuge (you will only see two layers)
Collect top layer: serum which contains everything except coagulation factors |
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Term
| Lavendar and Pink top tubes |
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Definition
plasma tube
Contains ethylenediaminetetraacetic acid
EDTA binds to calcium |
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Term
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Definition
plasma tube
Contains Heparin (anticoagulant) |
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Term
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Definition
plasma tube
Contains sodium citrate (anticoagulant) |
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Term
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Definition
| Chem, Hematology, Urine Microscopy, Serology, Microbiology. |
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Term
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Definition
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Term
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Definition
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Term
| Components of Clinical Chemistry |
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Definition
| Specimens, Reagents, instrumentation. |
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Term
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Definition
Wet kits: shorter shelf life, they have specific storage conditions
Dry kits: longer shelf life, come with a diluent |
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Term
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Definition
| free from ions, particles, microorganisms, etc. |
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Term
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Definition
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Term
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Definition
volume added/total volume, then take the inverse
Example: 1mL serum = 1 = Dilution Factor of 2
1 mL serum + 1 mL water 2 |
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Term
| Absorbance Spectrophotometry |
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Definition
| A spectrophotometer can be used to distinguish compounds by analyzing the pattern of wavelengths absorbed by a given sample |
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Term
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Definition
%Transmittance: amount of light transmitted through the chemical
A: absorbance – amount of light absorbed by the chemical
A = ebc
e: absorbance coefficent factor
b: width of the light path
c: concentration of the solution
Therefore: A = -log T or A = 1/ log T |
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Term
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Definition
| Helps to correlate known concentrations to unknown concentrations |
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Term
| How do you make a standard curve? |
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Definition
Do a serial dilution
Add reagent to dilutions, incubate, then take the absorbances and graph them |
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Term
| Flame Photometry (emission spectrophotometry) |
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Definition
| Different ions emit different colored light when excited |
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Term
| Atomic Absorption Spectrophotometry |
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Definition
Inverse of flame photometry
No excitation/ionization
Causes the atoms to be in a ground state
Measuring the light absorbed by the atoms |
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Term
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Definition
| Measuring fluorescent light |
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Term
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Definition
Amount of scattered light
Measuring the collision of molecules, how many molecules are present
Not measuring concentration
Kidneys |
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Term
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Definition
Measuring how much light was blocked
Usually used in determining bacterial growth |
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Term
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Definition
Osmolarity: measure of solute concentration, # of osmoles per solution
Used for urine and blood testing
Urine osmolality vs plasma osmolality
Please add more notes on this topic
Refractometer
Instrument that helps determine the concentration of a solution |
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Term
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Definition
Separation of molecules according to molecular weight and net charge with a buffering system
Tertiary structures of proteins are used
Three types of gels
Polyacrylamide
Starch
Agarose |
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Term
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Definition
| Measures the compostition of proteins |
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Term
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Definition
Measurement of electromotive potential
Example: ISE (ion selective electrode)
pH meter: selective for Hydrogen ions
Glucose meters are based on this technology |
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Term
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Definition
Competitive binding assay
You administer a radioactive labeled agent to a patient then evaluate how much you see in blood or urine |
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Term
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Definition
| Plasma-coagulation factors |
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Term
| Flame Emission Spec Colors |
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Definition
| NaY LiaRs KV MgB (Na=Yellow, Li=Red, K=Violet , Mg=Blue) |
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