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What Is Life (Part 2 & 3)
Vocabulary & Key Concepts
18
Biology
Undergraduate 1
09/20/2013

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Cards

Term
iodine test
Definition
starch; turns blue
Term
Benedict's test
Definition
reducing sugars; turns orange-red
Term
spectrophotometer
Definition
uses standard solutions to quantify qualitative color results
Term
fat & oil test
Definition
translucent spot appears on glazed paper
Term
Biuret's test
Definition
protein; turns red
Term
Feulgen's/Schiff's stain
Definition
stains DNA
Term
wet weight
Definition
live or fresh weight of an organism or tissue
Term
dry weight
Definition
weight after drying
Term
acetic acid
Definition
fixative; 45% aq; rapid; loss/distortion of proteins
Term
acetic alcohol
Definition
fixative; 3 parts 100% ethanol, 1 part glacial acetic acid; good for squashing
Term
air drying
Definition
fixative; single isolated cells; extreme distortion/unstable; doesn't extract any soluble molecules
Term
formaldehyde
Definition
fixative; 5% aq; preserves proteins; rigid
Term
freeze-drying
Definition
fixative; rapid/minimum distortion; instable (unless chemical fixation)
Term
freeze-substitution
Definition
fixative; rapidly frozen & then brought to room temperature; minimal distortion/movement; complex/rigid
Term
acetocarmine
Definition
stain; basic dye in acid solution; stains acidic compounds
Term
fast green
Definition
stain; pH2 = protein stain; pH8 = histone stain
Term
triphenyl tetrazolium chloride
Definition
VITAL stain; shows redox reaction
Term
phase contrast microscopy
Definition
can provide contrast without stains
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