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VT 242 - CBC Automation
N/A
13
Veterinary Medicine
Undergraduate 2
03/13/2013

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Term
A Complete Blood Count is indicated for:
Definition
-Diagnostic evaluation of any and all disease states
-Pre-surgical screening
-Well animal screening
Term
Blood tube should be filled to...
Definition
75-90%
Term
Advantages to in house automation.
Definition
-Sample is always fresh
-Immediate information
-Easy to follow the progression or regression of a disease
-Standardization
-Potential income intake
Term
Disadvantages to in house automation?
Definition
-Cost of the Equipment
-Cost of supplies
-Training of the technician
-Upkeep of the equipment
Term
Four types of CBC equipment?
Definition
-Hematocrit tube and blood film micro evaluation
-Quantitative buffy coat analyzers
-Impedence Analyzers
-Flow Cytometry
Term
How does a Quantitative buffy coat analyzer work? Simple
Definition
-Uses measurements from an expanded buffy coat.
- Stains agran.'s green and gran's orange/yellow
-Can only do a simple diff.
-Gives agran and gran %'s only
Term
Advantages to a buffy coat analyzers?
Definition
-easy to operate
-compact size of the machine
-low maintenance
-minimum consumables to track
-service is relatively inexpensive
Term
Disadvantages to a buffy coat analyzers?
Definition
-Requires live specimens
-highest cost per test
-slow turn around time - 10mins
-No quality control
-limited diagnostic application
Term
How does the impedance method/coulter counter principle work? Simple
Definition
-cells are passed through an opening containing electrical current created by two electrodes. Each cell that passes through the electrodes creates an interruption/impedance which is measured by the machine. The machine is set to measure within a certain threshold/range.
Term
Advantages to an impedance method machiens
Definition
-simple to use and maintain
-entry level units are very affordable
-low cost per test
-fast turn around
Term
Disadvantages to impedance method machines
Definition
-RBC/PLT overlaps
-Physical size of the instrument
-relatively expensive to service
-most inventory is dated like solutions and other consumables
Term
How does a flow cytometry machine work?
Definition
Cells pass as a single stream in front of a laser to determine the size and density of the cell. Some dyes can be added to detect immature cells
Term
List some abnormals not found on an auto CBC
Definition
-NRBC's
-mega platelets
-polychromasia
-any type of abnormal rbc morphology
-basophils
-etc etc etc......
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