+ssRNA

no capsid enzymes

replicates in cytoplasm

genome has VPg protein at 5', polyA tail 3' (fools ribosome into translating it)

Monosystronic mRNA is translated directly to polyprotein

codes for proteases, structural coat proteins, RNA replicase 

not QUITE eliminated worldwide, but close.

-ssRNA, enveloped

bullet-shaped capsid contains RNA-dependent RNA polymerase

replicates in cytoplasm -- forms envelope by budding out of the cell

Genome is used to make several +ssRNA/mRNA transcripts, each of which encodes a single protein

Genome is also transcribed completely into +ssRNA, then back to -ssRNA for replication

-ssRNA

RNA-dependent RNA polymerase

RNA endonuclease (removes 5' cap from cellular transcripts)

Replicates in the nucleus

Genome is segmented -- each of 8 encodes a different gene

Envelope proteins include HA (hemagglutinin -- spikes on structure that aid attachment) and NA (neuraminidase -- blocks hemagglutinin attachment)

antigenic shift is common

removes 5' caps on cell mRNAs

dsRNA

capsid contains RNA-dependent RNA polymerase

replicates in the cytoplasm

Genome is segmented, with some overlapping genes -- first AUG sometimes missed

There is also a template strand

Capsid is a double shell

leads to severe diarrhea in children

circular dsDNA genome

no capsid enzymes

replicates in nucleus

nonpermissive infection: infection results in viral replication

nonpermissive infection: becomes part of host genome, activities of T-antigen proteins involved (--> tumor formation)

dsDNA

no capsid enzymes

Site of replication: nucleus

replication by rolling circle mechanism

Transcription stages:

immediate early, delayed early (DNA replication enzymes), late (structural proteins)

virus buds through the inner nuclear membrane

RNA --> DNA --> host genome --> host gene expression

pre-HIV, NIH took shit for wanting to study retroviruses. Then AIDS hit and everyone was like "....oh."

enveloped

complex structure

HIV: phospholipid bilayer, protein shell, nucleocapsid (core protein), RNA genome (w/capsid proteins)

protease wtf. Actually I have no idea why this was in my notes, but I'm getting tired so it goes in here anyway.