Term
| feline panleucopaenia virus (FPV) = |
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Definition
systemic neonatal dz
cerebellar hypoplasia
enteritis leukopaenia |
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Term
| CPV 1 (canine parvovirus) = |
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Definition
neonatal deaths
myocarditis
repro failure
mild enteritis? |
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Term
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Definition
systemic neonatal dz
enteritis
myocarditis
leukopenia |
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Term
| porcine parvovirus (PPV) = |
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Definition
Stillbirths
Mummification
Eembryonic Death
= INFERTILITY |
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Term
| FPV and CPV are relatively rare noe, why? |
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Definition
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Term
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Definition
| icosahedral (made of 2 proteins) |
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Term
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Definition
ssDNA
v. small!
4ORFs - only encodes 4 proteins :
-NSP1
-NSP2
-VP1
-VP2
(VP = structural, capsid components) |
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Term
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Definition
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Term
| parvovirus particels are resistant to... |
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Definition
v stable
-resistant to heat
extreme pH
detergents
and some disinfectants |
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Term
| parvovirus is spread by... |
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Definition
| -spread by vets, feeding bowls, animal sheds |
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Term
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Definition
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Term
| CPV and FPC bind to what R on host cell? |
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Definition
| transferrin (widely expressed on dividing cells) |
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Term
| parvovirus is released into host cell cytoplasm by |
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Definition
| acidifictaion of endosome = activate phospholipid domain in capsi protein = detroy endosome mb and release capsid |
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Term
| when parvovirus at host cell nucleus it... |
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Definition
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Term
| replication of viral DNA by |
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Definition
| host DNA pol (only present in dividing cells) - turns ssDNA to dsDNA and then host RNA pol transcribes DNA to RNA |
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Term
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Definition
-bind to viral DNA and is essential for initiation of replication
-helicase: splits DNA strand so transcription can occur
-endonuclease = nicks virus DNA to make new viral genome to package, also nicks cellular DNA = arrests host cell in synthesis/ S phase= cell keeps replicating - stuck in S phase. This triggers apoptosis and virus release |
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Term
| CPV appeared in EU and spread around world within... |
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Definition
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Term
| why doesnt parvovirus mutate alot? |
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Definition
| replicate by hst cell enzymes |
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Term
| 5 clincial symptoms you can get with parvovirus = |
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Definition
1. generalised neonatal dz
2. leukopaenia
3. enteritis
4. cerebellar hypoplasia
5. myocarditis |
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Term
| generalised neonatal dz occurs because |
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Definition
| neonates have lots of dividing cells=infect all cells in neonate |
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Term
| age of D or C with generalised neonatal dz = |
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Definition
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Term
| leukopaenia/enteritis due to infection of... |
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Definition
...BM and crypt cells (constantly dividing cells)
usually seen in D and Cs 2-4months old |
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Term
| enteritis seen in D&Cs how old? |
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Definition
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Term
| what clincial sign tends to dominate in parvovirus inection in adult D&Cs |
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Definition
enteritis
-haemorrhagic enteritis, fever, bloody diarrhoea, vom
Pathological lesions with congestion and thickening in subacute cases |
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Term
| cerebellar hypoplasia occurs usually if... |
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Definition
...queen survived, infection of kittens with parvovirus
seen in kittens infected 2wks b4 birth to 4wks old |
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Term
| mycarditis occurs mainly in |
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Definition
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Term
| cerebellar hypoplasia occurs mainly in |
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Definition
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Term
| young puppies infected with parvovirus at 12days old = |
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Definition
| will get myocarditis, which can cause sudden death in puppies |
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Term
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Definition
1. virus shed in faeces
2. haemagglutination of swine RBCs
3. inhibiton by specific antisera
4. PCR (gd as genome = DNA NOT RNA = no reverse transcriptase needed, = more reliabel - less chance of mutation occuring :))
4. virus isolation in cell culture
5. Ag capture ELISA test using faecal sample |
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Term
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Definition
live attenuated and inactivated vacc against CPV and FPV
ubiquitous use of vacc |
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Term
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Definition
| ...protect neonate but in hibit vacc |
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Term
| what signs in a puppy or kitten may you worry about CPV/FPV infection? |
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Definition
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Term
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Definition
| embryo (crosses placenta) |
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Term
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Definition
| worlwide and endemic in many herds |
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Term
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Definition
Stillbirths
Mummification
Embryonic Death
= INFERTILITY |
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Term
| how well does PPV persost in enviro? |
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Definition
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Term
| after recovery PPV shedding = |
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Definition
| can carry on for several months! |
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Term
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Definition
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Term
| what pig is at risk of PPV |
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Definition
| gilts - maternal Abs protect from infection UNTIL 1st preg is established |
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Term
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Definition
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