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Urinalysis Confirmatory Testing
Urinalysis Confirmatory Testing
24
Medical
Undergraduate 2
03/04/2012

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Term
Protein-Sulfosalicylic Acid (SSA) Test Definition
Definition

confirmatory test for protein

 

used to confirm protein values when reagent strip values are in question

Term
SSA Test Principle
Definition
based on cold precipitation of protein with a strong acid
Term
SSA Test Specificity
Definition
free SSA in the working reagent precipitates protein in the specimen
Term
SSA Test Results
Definition

negative, no precipitate

trace, faint white precipitate

1+, turbid

2+, can't read through but can see lines

3+, can't see through, granules might be present

4+, gelled tube

Term
SSA Test Sensitivity
Definition
5 - 10 mg/dL
Term
SSA Test False Positives
Definition

radiographic dyes

 

penicillin

Term
SSA Test False Negatives
Definition

highly buffered urine

 

alkaline urine

Term
Protein Precipitation by Heat Test Principle
Definition
free light chains (Bence Jones protein) are soluble at 100C and precipitate at 60C
Term
Protein Precipitation by Heat Test Normal Result
Definition
negative
Term
Protein Precipitation by Heat Test Sensitivity
Definition
20 mg/dL
Term
Glucose-Copper Reduction Test Specificity
Definition
reducing substances: glucose, galactose, lactose
Term
Glucose-Copper Reduction Test Principle
Definition
CuSo4 + reduced substance --> CuOH + Cu2O + oxidized substance
Term
Glucose-Copper Reduction Test Sensitivity
Definition
250 mg/dL
Term
Glucose-Copper Reduction Test False Positive
Definition
acorbic acid
Term
Glucose-Copper Reduction Test Nickname
Definition
Clinitest
Term
Ketones-Nitroprusside Test Principal
Definition
acetoacetic acid + Na nitroprusside gives a purple color
Term
Ketone-Nitroprusside Test Sensitivity
Definition
5 - 10 mg/dL acetoacetic acid
Term
Ketone-Nitroprusside Test False Negative
Definition
breakdown of acetoacetate by bacteria
Term
Ketone-Nitroprusside Test Nickname
Definition
Acetest
Term
Bilirubin-Diazo Tablet Test Principle
Definition
conjugated bilirubin + diazo reagent --> azobilirubin
Term
Bilirbun-Diazo Tablet Test Normal Result
Definition
negative
Term
Bilirubin-Diazo Tablet Test Sensitivity
Definition
0.05 - 0.1 mg/dL
Term
Bilirubin-Diazo Tablet Test Specificity
Definition
conjugated bilirubin
Term
Bilirubin-Diazo Tablet Test False Negatives
Definition

vitamin C

 

photo oxidation

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