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Unit Test 2 Epstein
Lecture 6-11
127
Biology
Undergraduate 1
03/04/2009

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Term
How do enzymes catalyze biochemical reactions? 1.
Definition
1. Orient substrates for their proper interaction.
Term
How do enzymes catalyze biochemical reactions? 2.
Definition
2. Stress or bend substrate bonds to get them closer to transition state.
Term
How do enzymes catalyze biochemical reactions? 3.
Definition
3. Provide a favorable microenvironment (acidic and basic groups in active site) that facilitates the reaction.
Term
How do enzymes catalyze biochemical reactions? 4.
Definition
4. Covalent bonding of enzyme to substrate; enzyme becomes part of the transition state, but later released intact.
Term
Blocks substrate so that enzyme cannot properly bond.
Definition
competitive inhibitor
Term
impedes enzymatic reactions without directly competing with substrate for enzyme bonding
Definition
non competitive inhibitor
Term
when a regulatory molecule binds to a protein at one site and affects the protein’s function at another site
Definition
Allosteric regulation
Term
Allosteric activator
Definition
stabilizes the shape that has functional active sites
Term
noncompetitive inhibitor
Definition
stabilizes the inactive form of the enzyme
Term
cooperativity
Definition
form of allosteric regulation that can amplify enzyme activity. In cooperativity, binding of a substrate to one active site stabilizes favorable conformational changes at the other active sites.
Term
Which inhibitor is more potent?
Definition
Noncompetitive inhibitors are more potent because they cannot be overpowered by an increased amount of substrate.
Term
How can a competitive inhibitor be overpowered?
Definition
A competitive inhibitor can be overpowered by increasing the concentration of the substrate.
Term
How does enrve gas effect the nervous system?
Definition
Acetylcholine binds to ion channels allowing the flow of ions making an electrical impulse. nerve gas binds to acetylcholine covalently which blocks ligand gated ion channels, not allowing acetylcholine to bind.
Term
partial degradation of sugars that occurs without the use of oxygen
Definition
fermentation
Term
The most prevalent and efficient catabolic pathway
Definition
aerobic respiration
Term
aerobic respiration
Definition
oxygen is consumed as a reactant along with organic fuel
Term
Transfer of one or more electrons from on reactant to another
Definition
redox reaction
Term
loss of electrons from one substance during a redox reaction
Definition
oxidation
Term
addition of electrons to a substance in redox reactions
Definition
reduction
Term
the electron donor in a redox reaction
Definition
reducing agent
Term
the electron acceptor in a redox reaction
Definition
oxidizing agent
Term
tubules in the mitochondria
Definition
cristae
Term
sacs in the golgi
Definition
golgi stacks
Term
sacs in the ER
Definition
cisternae
Term
VACUOLES HOLD FOUR THINGS
Definition
1. PROTEINS
2. INORGANI IONS
3. METABOLIC BYPRODUCTS
4. PIGMENTS
Term
LOCATION OF RIBOSOMAL SUBUNITS
Definition
NUCLEOLUS
Term
RIBOSOMES BUILD _______
Definition
PROTEINS
Term
NETWORKS WITHIN THE ENDOPLASMIC RETICULUM
Definition
LUMEN
Term
THE MEMBRANE FACTORY OF THE CELL
Definition
ROUGH ER
Term
THE ROUGH ER CARRIES OUT
Definition
1. SYNTHESIS OF MEMBRANE PROTEINS
2. SECRETORY PROTEINS
3. SITE OF GLYCOSYLATION
Term
THE SMOOTH ER FUNCTIONS AS
Definition
THE LOCATIONS OF SYNTHESIS OF LIPIDS
DETOX OF POISONS
STORAGE OF CALCIUM
Term
TERM DESCRIBING SOMETHING THAT BREAKS THINGS DOWN INTO ITS SIMPLEST UNITS
Definition
HYDROLYTIC
Term
FUNCTION OF PEROXISOME
Definition
BREAKS THINGS DOWN EX.) FATTY ACIDS INTO HYDROGEN
Term
SACS OF HYDROLYTIC FAT ENZYMES THAT ANIMAL CELLS USE TO DIGEST MACROMOLECULES
Definition
LYSOSOMES
Term
PHAGOCYTOSIS
Definition
AMOEBA AND BACTERIA ENGULFS FOOD- THE FOOD BECOMES A FOOD VACUOLE- IT FUSES WITH A LYSOSOME- THE LYSOSOME BREAKS DOWN FOOD AND THE DIGESTED PRODUCTS PASS INTO THE CELL TO BECOME NUTRIENTS
Term
AUTOPHAGY
Definition
THE RECYCLING OF A CELLS OWN MATERIAL

DAMAGED ORGANELLS ACQUIRES A DOUBLE MEMBRANE- LYSOSOME FUSES WITH OUTER MEMBRANE- LYSOSOMAL ENZYMES DISMANTLE MATERIAL AND RETURN IT TO CELL
Term
PATH OF A TRANSPORT VESICLE
Definition
BUDS OFF OF ROUGH ER- ENTER CIS GOLGI- EXITS TRANS GOLGI AFTER A MODIFICATION OF PRODUCTS- EXITS PLASMA MEMBRANE- FUSES WITH LYSOSOME
Term
WHAT ARE MICROTUBULES MADE OF?
Definition
13 COLUMNS OF TUBULIN
Term
WHAT COMPRISES TUBULIN?
Definition
IT IS A PROTEIN MADE UP OF A DIMER, AN A-TUBULIN AND A B-TUBULIN
Term
MAKES MICROTUBULES BEND
Definition
DYNEINS
Term
9+2?
Definition
9 DOUBLETS OF MICROTUBULES AND 2 CENTER MICROTUBULES, ATTACHED BY DYENINS
Term
DOUBLETS ARE ATTACHED BY
Definition
CROSS LINKING PROTEINS
Term
WHAT MAKES UP MICROFILAMENTS?
Definition
2 STRANDS OF ACTIN
Term
WHAT PURPOSE DO MICROFILAMENTS SERVE?
Definition
MAINTAINING CELL SHAPE, MUSCLE CONTRACTION, CYTOPLASMIC STREAMING, CELL DIVISION
Term
WHAT PURPOSE DO MICROTUBULES SERVE?
Definition
SHAPE CELL, CELL MOTILITY, ORGANELLE MOVEMENT, CHROMOSOME MOVEMENT IN CELL DIVISION
Term
WHAT MOTOR PROTEIN HELPS MUSCLES MOVE?
Definition
MYOSIN
Term
HOW DO MUSCLES CONTRACT?
Definition
MULITPLE STRANDS OF ACTIN BOUND BY MYOSIN SLIDE PAST ONE ANOTHER
Term
WHAT IS THE FUNCTION OF INTERMEDIATE FILAMENTS?
Definition
CELL SHAPE
ANCHORAGE OF NUCLEAUS AND OTHER ORGANELLES
Term
WHAT ARE INTERMEDIATE FILAMENTS PROTEINS?
Definition
KERATINS
Term
WHAT IS THE SIZE OF EACH COMPONENT OF THE CYTOSKELETON?
Definition
MICROTUB- 25
MICROFIL- 7
INTERM.- 8-12
Term
WHAT IS THE CORE OF THE CELL MEMBRANE MADE OF?
Definition
PHOSPHOLIPID BILAYER
Term
WHAT IS FLUIDITY EFFECTED BY?
Definition
KINKS AT THE DOUBLE BONDS OF HYDROCARBON TAILS
Term
THE MORE FLUID THE MORE ________
Definition
UNSATURATED
Term
THE MORE VISCOUS THE MORE ______
Definition
SATURATED
Term
WHAT DOES CHOLESTEROL DO TO MEMBRANES?
Definition
CHOLESTEROL MAKES MEMBRANES LESS FLUID BY RESTRAINING PHOSPHOLIPID MOVEMENT.
Term
INTEGRAL PROTEINS
Definition
PENTRATE THE HYDROPHOBIC CORE
Term
FOUR TYPES OF INTERGRAL PROTEINS-
Definition
TRANSPORT, SIGNAL TRANSDUCTION, CELL-CELL RECOGNITION, INTERCELLULAR JOINING
Term
SIMPLE DIFFUSION
Definition
USES NO ENERGY, FLOW DOWN CONCENTRATION GRADIENT
Term
DIFFUSION OF WATER
Definition
OSMOSIS
Term
IN A HYPOTONIC SOLUTION A CELL WILL
Definition
GAIN WATER AND BURST
Term
IN A HYPERTONIC SOLUTION
Definition
A CELL WILL LOSE WATER AND SHRIVEL
Term
A PLANT CELL IS TURGID IN
Definition
A HYPOTONIC SOLUTION
Term
A PLANT CELL IS PLASMOLYZED IN A
Definition
HYPERTONIC SOLUTION
Term
A PLANT CELL IS FLACCID IN A
Definition
ISOTONIC SOLUTION
Term
ACTIVE TRANSPORT
Definition
REQUIRES ENERGY, AGAINST CONCENTRATION GRADIENT, ENCOMPASSES ALL CARRIER PROTEINS,
Term
ATP SUPPLIES ENERGY IN ACTIVE TRANSPORT BY
Definition
TRANSFERRING A PHOSPHATE GROUP TO A TRANSFER PROTEIN SO THE PROTEIN CHANGES SHAPE AND LOCATES THE MOLECULE ACROSS THE MEMBRANE
Term
VOLTAGE
Definition
DIFFERING ION CONCENTRATIONS ON EACH SIDE OF THE MEMBRANE MAKE DIFFERENT CHARGES TO EACH SIDE
Term
AMOUNT OF VOLTAGE ACROSS A MEMBRANE
Definition
MEMBRANE POTENTIAL
Term
HOW DOES A SODIUM POTASSIUM PUMP WORK?
Definition
THE SODIUM POTASSIUM PUMP PUMPS THREE SODIUM IONS PER EVERY TWO POTASSIUM ION. THE TRANSFER IS 1 POSITIVE CHARGE, STORING ENERGY AS VOLTAGE.
Term
SODIUM POTASSIUM PUMP IS AN EXAMPLE OF A
Definition
ELECTROGENIC PUMP, THE MAJOR PUMP IN ANIMAL CELLS
Term
WHAT IS THE MJOR PUMP IN PLANT CELLS?
Definition
PROTEIN PUMP
Term
"CELLULAR DRINKING"
Definition
PINOCYTOSIS, CELLG GULPS EXTRACELLULAR FLUID AND ABSORBS DISSOLVED MOLECULES
Term
RECEPTOR MEDIATED ENODCYTOSIS
Definition
PROTEINS WITH RECEPTORS EXPOSED TO EXTRACELLULAR FLUID CONCETRATE IN A PIT OF LIGANDS- VESICLE WITH LIGAND MOLECULES FORM- MATERIAL FREED FROM VESICLE AND THE RECEPTORS ARE RECYCLED
Term
SPECIFICALLY BINDS TO PROTEIN
Definition
LIGAND
Term
METABOLIC REACTION THAT RELEASES ENERGY BY BREAKING DOWN MOLECULES
Definition
CATABOLIC
Term
METABOLIC REACTION THAT CONSUMES ENERGY BY BUILDING COMPLEX MOLECULES
Definition
ANABOLIC
Term
A NEGATIVE DELTA G MEANS THAT THE REACTION CAN OCCUR______
Definition
SPONTANEOUSLY
Term
WHEN THERE HAS BEEN A NET LOOS OF ENERGY A REACTION CAN OCCUR
Definition
SPONTANEOUSLY
Term
TOTAL ENERGY IN A SYSTEM
Definition
ENTHALPY
Term
AMOUNT AVAILABLE TO DO WORK
Definition
GIBBS FREE ENERGY
Term
MEASURE OF CHAOS IN A SYSTEM
Definition
ENTROPY
Term
ACRONYM FOR ENTHALPY
ACRONYM FOR GIBBS FREE ENERGY
ACRONYM FOR ENTROPY
Definition
H
G
S
Term
EQUATION FOR THERMODYNAMICS
Definition
DELTA G= DELTA - T DELTA S
Term
A CELL DOES THREE TYPES OF WORK-
Definition
CHEMICAL
TRANSPORT
MECHANICAL
Term
ATP IS MADE UP OF
Definition
RIBOSE+ADENINE+ CHAIN OF 3 PHOSPHATES
Term
SYNTHESIS OF CELL MATERIALS AND COMPONENTS
Definition
CHEMICAL WORK
Term
EX. PUMPING CELLS ACROSS A MEMBRANE
Definition
TRANSPORT WORK
Term
MOVEMENT OF CELLS OR CELLULAR MATERIAL
Definition
MECHANICAL WORK
Term
WHAT ARE THE 3 COMPONENTS OF CELLULAR RESPIRATION?
Definition
GLYCOLYSIS, THE CITRIC ACID CYCLE, OXIDATIVE PHOSPHORYLATION
Term
Cellular respiration is also based on redox reactions and the transfer of electrons from states of ----- stability (----- potential energy) to states of ----- stability (----- potential energy).
Definition
Cellular respiration is also based on redox reactions and the transfer of electrons from states of low stability (high potential energy) to states of high stability (low potential energy).
Term
Begins cellular respiration
Definition
glycolysis
Term
How does glycolysis being the degradation process?
Definition
in the cytosol, it breaks glucose into two molecules of the compound pyruvate
Term
What completes the breakdown of glucose?
Definition
CITRIC ACID CYCLE
Term
WHAT DOES THE CITRIC ACID CYCLE DO? WHERE DOES THIS OCCUR?
Definition
OXIDIZES A DERIVATIVE OF PYRUVATE TO CARBON DIOXIDE. IN THE MITOCHONDIRAL MATRIX OR CYTOSOL FOR PROKARYOTES
Term
HOW IS ATP ENERGY ACQUIRED?
Definition
DURING THE BREAKDOWN OF ELECTRON TRANSPORT CHAINS, THE PASSING OF ELECTRONS ALONG MOLECULES. STORED IN THE MITOCHONDRION
Term
WHAT IS ATP SYNTHESIS CALLED? WHY?
Definition
OXIDATIVE PHOSPHORYLATION, IT IS POWERED BY THE REDOX REACTIONS OF THE ELECTRON TRANSPORT CHAIN
Term
What is essentially an intermediate in energy transfer and the production of ATP?
Definition
NAD+ is an “electron carrier” and is essentially an intermediate in energy transfer and the production of ATP
Term
HOW DO GLYCOLYSIS REACTIONS OCCUR?
Definition
EACH IS TRIGGERED BY A DIFFERENT ENZYMES
Term
WHAT IS THE RESULT OF GLYCOLYSIS?
Definition
Result is to break down glucose, a 6-carbon sugar, to two molecules of pyruvate, a 3-carbon compound.
Term
WHAT WILL BE EXTRACTED IN THE GLYCOLYSIS PROCESS?
Definition
In process extract energy to make 4 molecules ATP and 2 molecules of NADH.
Term
WHAT IS ATP GENERATED BY IN GLYCOLYSIS?
Definition
substrate level phosphorylation.
Term
HOW DO YOU TRANSCEND FROM GLYCOLYSIS TO THE CITRIC ACID CYCLE?
Definition
To get from glycolysis to TCA cycle, pyruvate is transported into mitochondria.
Term
WHAT HAPPENS IN THE PROCESS OF GETTING FROM GLYCOLYSIS TO THE CITRIC ACID CYCLE?
Definition
In the process, generate 2 more molecules of NADH per starting molecule of glucose (1 for each of the two pyruvates).
Term
WHAT HAPPENS DURING THE CITRIC ACID CYCLE?
Definition
Acetyl CoA (2 carbons) combines with oxaloacetate (4 carbons) to form citrate (6 carbons)

Citrate broken down, in 7 stepwise reactions, to regenerate oxaloacetate.

The other 2 carbons are oxidized to CO2.

This generates energy used to form 1 ATP, 3 NADH, and 1 FADH2 (another electron carrier).

From original molecule of glucose, yield is 2 ATP, 6 NADH, 2 FADH2
Term
WHAT DOES THE ELECTRON TRANSPORT CHAIN CONSIST OF?
Definition
The electron transport chain consists of 4 multi-protein complexes embedded in the inner membrane of the mitochondria.
Term
WHY ARE MORE ATPS GENERATED FROM NADH THEN FROM FADH2?
Definition
NADH feeds its electrons into chain at complex I; FADH2 feeds electrons into chain at complex II.
Term
CHEMIOSMOSIS
Definition
ENERGY STORED IN THE FORM OF A HYDROGEN ION GRADIENT ACROSS A MEMBRANE IS USED TO DRIVE CELLULAR WORK SUCH AS THE SYNTHESIS OF ATP
Term
MOST OF THE NADH and FADH2 is generated IN THE
Definition
MITOCHONDRIAL MATRIX
Term
THE ELECTRON TRANSPORT TRAIN IN CELLULAR RESPIRATION IS LOCATED IN THE
Definition
INNER MITOCHONDIRAL MEMBRANE
Term
WHAT IS THE FUNCTION OF THE ELECTRON TRANSPORT CHAIN?
Definition
TO ESTABLISH THE HYDROGEN ION GRADIENT USING THE EXERGONIC FLOW OF ELECTRONS FROM NADH AND FADH2 TO PUMP HYDROGEN IONS ACROSS THE MEMBRANE FROM THE MITOCHONDRIAL MATRIX TO THE INTERMEMBRANE SPACE.
Term
PROTON-MOTIVE FORCE
Definition
HYDROGEN ION GRADIENT RESULTING FROM THE DEPOSIT OF HYDROGEN IONS INTO THE INTERMEMBRANE SPACE
Term
ENERGY- COUPLING MECHANISM THAT USES ENERGY STORED IN THE FORM OF H+ GRADIENT ACROSS A MEMBRANE TO DRIVE CELLULAR WORK
Definition
CHEMIOSMOSIS
Term
This complex of proteins provides a tunnel for the passage of H+ ions as they move along their concentration gradient.
Definition
ATP SYNTHASE
Term
AS H+ IONS FLOW THROUGH THE ENZYME RESULTING IN
Definition
But as they flow through the enzyme, they turn a rotor, that turns the internal rod, which generates the energy for the catalytic knob component to convert ADP to ATP.
Term
ALTERNATIVE PATHWAY FOR ENERGY PRODUCTION WHEN OXYGEN IS SCARCE
Definition
Fermentation is an alternative pathway for energy production that is used when oxygen is scarce.
Term
ALCOHOL FERMENTATION
Definition
PYRUVATE-->ETHANOL
TWO STEPS:
RELEASE CARBON DIOXIDE FROM PYRUVATE WHICH IS CONVERTED TO ACETALDEHYDE.
ACETYLDEHYDE IS REDUCED BY NADH TO ETHANOL.
Term
LACTIC ACID FERMENTATION
Definition
PYRUVATE IS REDUCED DIRECTLY TO NADH TO FORM LACTATE AS AN END PRODUCT WITH NO RELEASE OF CO2
Term
FACULTATIVE ANAEROBES
Definition
ORGANISM THAT CAN MAKE ENOUGH ATP TO SURVIVE USING EITHER FERMENTATION OF RESPIRATION
Term
OLIGATE ANAEROBES
Definition
ORGANISM CARRYING OUT ONLY FERMENTATION OR ANAEROBIC REPSPIRATION, CANNOT SURVIVE IN PRESENSE OF OXYGEN.
Term
NAD+
Definition
HYDROGEN ATOMS ARE NOT TRANSFERRED DIRECTLY TO OXYGEN, INSTEAD THEY ARE PASSED TO THIS ELECTRON CARRIER.
Term
ACETLYL COA
Definition
PYRUVATE IS CONVERTED TO THIS UPON ENTERING THE MITOCHONDRION VIA ACTIVE TRANSPORT.
Term
PROTEIN. ELECTRON CARRIERS BETWEEN UBIQUIONE AND OXYGEN
Definition
CYTOCHROMES
Term
AN ENZYME THAT MAKES ATP FROM ADP AND INORGANIC PHOSPHATE
Definition
ATP SYTHASE
Term
OXYGEN IS CONSUMED AS A REACTANT ALONG WITH THE ORGANIC FUEL
Definition
AEROBIC RESPIRATION
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