Shared Flashcard Set

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Unit 4 Lab
also with Unit 4 Lecture
16
Microbiology
02/10/2012

Additional Microbiology Flashcards

 


 

Cards

Term
_____ is defined as the shape and arrangement of a colony.
Definition
Colonial morphology
Term
_____ is used to describe the shape and arrangement of the individual bacterial cells.
Definition
Cellular morphology
Term
What may be learned when performing a gram stain?
Definition
cellular morphology and gram reaction
Term
What is the primary or first stain used in the Gram stain procedure?
Definition
crystal violet
Term
What is the function of Gram's Iodine?
Definition
mordant
Term
What is the function of alcohol when performing the gram stain?
Definition
decolorizer
Term
This is the type of cell that has a lot of lipids in its cell wall construction.
Definition
Gram negative
Term
What is used to adhere and preserve your microbial cells to the glass slide?
Definition
passing the dry slide over an open flame will adhere the organisms to the slide.
Term
How old should your culture be when performing a Gram Stain?
Definition
24-48 hour old cultures
Term
What happens if you de-colorize a gram stain too long?
Definition
all of the cells will be red
Term
This is a type of bacterial morphology described as complete helices
Definition
spirilla
Term
This is the counter-stain used in the Gram staining procedure.
Definition
safranin
Term
This is a colonial characteristic exemplified by convex, pulvinate, umbonate, and flat appearances.
Definition
elevation
Term
This may result if performing a gram stain on an old culture
Definition
false gram negative results
Term
What is the purpose of adding Lugol's Iodine when performing the Gram Stain?
Definition
Lugol's Iodine complexes with the crystal  violet to form larger molecules which are more easily retained within the cell wall.
Term
This is the decolorizing agent used in the Gram staining procedure.
Definition
95% ethyl alcohol