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Unit 1 Lab
Microscopes
15
Microbiology
02/07/2012

Additional Microbiology Flashcards

 


 

Cards

Term

Why was it necessary to move the letter "e" to the center of the low power field before changing to high power?

 

 

Definition
to keep the image in your field of view
Term
How does the microscope change the position of the object being observed?
Definition
The object is upside down and backwards in orientation.
Term
What is the total magnification of objects observed through a 40x high power objective with a 7.5x ocular?
Definition
300X
Term
If you were getting a total magnification of 270x while using a 45x objective, what would be the power of the eyepiece?
Definition
6x
Term
Trace the path of light through a microscope numbering each part of the microscope  as the light passes through from the light to the oculars.
Definition

1.  light source

2.  diaphragm

3.  condenser

4.  stage

5.  objectives

6.  revolving nose piece

7.  body tube

8.  ocular

Term
To bring your specimen into focus when using the high power objective, which focusing knob should you use?
Definition
fine adjustment
Term
Explain this statment: With high power you see more of less
Definition
You see less of the slide area but more specific detail
Term

Base: microscope rests on this part

Coarse adjustment: used to initially focus the microscope

Ocular: magnification and viewing of the specimen

Revolving Nose Piece: hold the objectives and allows you to change magnifications

Stage: a platform that holds the specimen/slide for viewing

Condenser: directs the path of light up into a straighter, more concentrated beam that enters into the lenses of the microscope

Definition
Term

Diaphragm: regulates the amount of light entering into the lenses of the microscope

Objectives: magnification

Arm: support the upper part of the microscope

Light: provides illumination fo the specimen

Body Tube: holds the oculars and directs the light to the eye

Stage Clips: hold the speimen/slide in place

Definition
Term
The smallest distance between two objects that allows the objects to be distinguished as two separate objects and not as one
Definition
resolution
Term
As magnification increases
Definition
the field of view decreases
Term
Magnification
Definition
is due to the outward bending of light rays as they pass through a lens
Term
Once a microscope has been initially focused, the ability of this scope to stay focused when changing to a higher magnification is
Definition
parfocal
Term
The actual amount of area on a slide that can be viewed under the microscope at one time without having to move the slide
Definition
field of view
Term
The distance from the specimen on the slide to the objective lens of the microscope, when the specimen is in focus
Definition
working distance