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Biology 200 Lecture Notes
11
Biology
Undergraduate 2
07/20/2011

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Term
Define transcription.
Definition
The synthesis of an mRNA version of the instructions stored in DNA.
Term
________ performs transcription by transcribing only one strand of DNA, called the _________.
Definition
RNA polymerase; template strand.
Term
What is the DNA strand not transcribed by RNA polymerase?
Definition
The non-template strand; sequence matches the sequence of RNA, but Us instead of Ts.
Term
What are the three phases of transcription?
Definition
Initiation, elongation, termination.
Term
RNA polymerase catalyzes the formation of ______________ between _____________.
Definition
Phosphodiester bonds; ribonucleotides.
Term
After DNA has been pulled apart and used as a template for creating a new mRNA, what happens to the DNA?
Definition
The hydrogen bonds that form between matching base pairs provide plenty of energy to pull the strands of DNA back together very quickly.
Term
Why is the new mRNA transcript constructed in the 5' to 3' direction?
Definition
The 3' OH is reactive and can form new bonds.
Term
What does initiation require?
Definition
Promoter-binding factors like sigma.
Term
What does elongation use to guide RNA?
Definition
DNA-RNA basepairing.
Term
What does termination use in the new RNA?
Definition
Hairpin structures.
Term
In bacteria, transcription and translation are __________.
Definition
Tightly coupled.
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