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Topic 3
Membranes
27
Biology
Undergraduate 1
02/22/2012

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Term
Functions of Membranes
Definition

Isolate, organize, and protect contents from the external environment.

Move materials in an out.

Convert energy to a usefull form.

Detect stimuli.

Provide for cell-to-cell communication.

Term
Phospholipid Bilayer
Definition
Two layers of phospholipids that are held together by the hydrophobic interactions of the fatty acids.
Term
Membrane Fluidity
Definition

Phospholipids exhibit lateral and flip-flop (initiated by enzymes called flippases) movement. Fluidity of membranes is influenced by the number of saturated and unsaterated fatty acids in the membrane. To offset the effects of high/low temperatures the cell will increase/decrease the length of the fatty acids and decrease/increase the proportion of unsaterated fatty acids. Cholestral can also stabilize membranes.

 

Term
Monolayer
Definition
Single chain that links the surfaces of the membrane (present in many members of the kingdom Archae).
Term
Fluid Mosaic
Definition

Membranes contain a variety of proteins that can move fluidly around the membrane.

  • Integral (intrensic) Proteins: Have a region that is in contact with the environment and a region that is in contact with the fatty acids.
  • Peripheral (extrensic) Proteins: Are on the surface of the membrane.
Term
Inetegral Proteins
Definition
Have a hydrophilic and a hydrophobic region. Some are "transmembrane proteins" and span the membrane.
Term
Glycoconjugates
Definition
Carbohydrates that are combined with either membrane lipids or proteins (are recognition molecules in man).
Term
Movement Through Phospholipid Bilayer
Definition
Membranes will allow gases, hydrophobic molecules, and small polar molecules to pass through, but large polar molecules and charged molecules cannot pass through.
Term
Aquaporin
Definition
Transmembrane protein that only allows water to pass through.
Term
Mechanisms for Crossing Membranes
Definition

Passive Transport

  • Osmosis: movement of water.
  • Diffusion: Movement of solute.
  • Facilitated Diffusion

Active transport: Requires energy.

Bulk Trasnport: Transports large molecules.

Term
Passive Transport
Definition
Molecules move from regions of higher concentration to regions of lower concentration (move down the "concentration gradient"). The greater the gradient the faster the molecules move. Molecules move independently of each other and stop moving whtn equilibrium is reached.
Term
Osmosis
Definition

Results in equalization of concentration and changes in volumes.  THe presence of charged solutes will reduce the rate of osmosis. Three types of environments:

Hypertonic (higher concentration than cell)

Isotonic (same concentration than cell)

Hypotonic (lower concentration than cell)

 

Term
Facilitated Diffusion
Definition

Movement is still down a concentration gradient but proteins are increasing the rate of diffusion (facilitated is min. 1000 times faster than passive). Two types of proteins involved:

Carrier Proteins: Undergo changes in shape to carry solute.

Channel Proteins: Posses central pore for passage of solute (Gated Channels are channel proteins that can open and close via a stimulus).

Term
ATP-Synthase
Definition
One of the most important proteins in facilitated transport. Uses the diffusion of protons to sythesize ATP.
Term
Saturated
Definition
When protein has reached the max. rate of facilitated diffusion.
Term
Active Transport
Definition
Moving molecules from low to high concentration via the use of energy. Direct active transport uses ATP and indirect active trasport uses other energy sources.
Term
Sodium-Potassium Pump
Definition
Active trasnport proein that moves two different substances in opposite directions (known as an antiporter). Process changes the membrane potential (and is thus electrogenic).
Term
Indirect Active Transport
Definition
Most use an ion gradient that is estiblished by active trasnport as their energy source. Most indirect transport systems are "coupled" with active transport systems.
Term
Sucrose Transporter
Definition
Inderect active transport system that uses a proton gradient that is established by a proton pump as its energy source. THe protons re-enter the cell by facilitated diffusion causing the protein to import sucrose.
Term
Uniporter
Definition
Moves one substance in one direction (proton pump).
Term
Symporter
Definition
Moves two different subtances in the same direction (sucrose transport).
Term
Antiporter
Definition
Moves two different substances in opposite directions (sodium-potassium pump).
Term
Bulk Transport
Definition

Moves large particles across a membrane.

Endocytosis: movement into cell

Exocytosis: movement out of cell (material is packaged into vesicles, the vesicles then fuse with the cell wall and release their contents outside of the cell).

Term
Endocytosis
Definition

Devided into:

Phagocytosis

Pinocytosis

Receptor-Mediated Endocytosis

Term
Phagocytosis
Definition
Non-specific mechanism that brings large particles into the cell.  The cell extends pseudopodia which surround and enclose particles in a vacuole.
Term
Pinocytosis
Definition
Non-specific mechanism that brings dissolves molecules into the cell through invagination.
Term
Receptor-Mediated Endocytosis
Definition
Mechanism only brings specific dissolved substances into the cell. When a substance binds to certain recptors the protein clathrin binds to the inner region of those receptors. THe clathrin causes the membrane to pull in, forming a "coated pit", and eventually a "coated vesicle".
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