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The Central Dogma: How DNA codes for traits
RNA, transcription, translation, DNA mutations
24
Biology
9th Grade
03/20/2012

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Term
RNA
Definition
ribonucleic acid
Term
compared to DNA, RNA...
Definition
  • is single-stranded
  • contains ribose sugar instead of deoxyribose
  • contains uracil instead of thymine
Term
messenger RNA
Definition
(mRNA) carries a code from DNA
Term
transfer RNA
Definition
(tRNA) carries an amino acid
Term
ribosomal RNA
Definition
(rRNA) makes up ribosomes
Term
codon
Definition
sequence of three nucleotides which together form a unit that codes for an amino acid
Term
anticodon
Definition
a sequence of three bases of a tRNA that are complementary to a codon in the mRNA
Term
Transcription definition and 3 steps...
Definition

In the nucleus, one gene on DNA is used to code for mRNA, which will be used in a separate process to make protein.

  1. gene activation
  2. making mRNA
  3. splicing
Term
Transcription: Step 1: Gene Activation
Definition
genes are activated by factors that allow the gene to unravel from its tightly coiled state and be transcribed into mRNA
Term
Transcription: Step 2: make mRNA
Definition

RNA polymerase is the enzyme that makes mRNA by:

  • binding to DNA at the start of the gene sequence
  • separating the strands of DNA
  • adding complementary RNA nucleotides based on sequence of DNA
  • proofreading
Term
Transcription: Step 3: splicing
Definition
  • add a cap to one end of pre-mRNA
  • add tail to other end of pre-mRNA (cap and tail protect it)
  • splice out introns (not needed sequences)
  • link together exons (needed sequences for coding for proteins)
  • mRNA leaves nucleus
Term
Translation's 3 steps...
Definition
  1. initiation
  2. elongation
  3. termination
Term
Translation: Step 1: Initiation
Definition
Translation begins with the mRNA "start" codon AUG. The first tRNA has the anticodon UAC and amino acid methionine.
Term
Translation: Step 2: Elongation
Definition
  • the 2nd mRNA codon matches a 2nd tRNA anticodon, which carries the 2nd amino acid
  • a peptide bond (a kind of covalent bond) forms between the two amino acids
  • the process is repeated several times
Term
Translation: Step 3: Termination
Definition
A "stop" codon, or a codon that does not code for any amio acid, is reached, and the protein is released.
Term
Translation
Definition
[image]
Term
gene mutations
Definition
mutations in DNA that affect only one gene
Term
point mutation
Definition
(a gene mutation) (aka substitution) changes one nucleotide
Term
frameshift mutation
Definition
(a gene mutation) changes the codon reading frame via a deletion or insertion of a nucleotide
Term
chromosomal mutations
Definition
mutations in DNA that affect a whole segment of a chromosome, and therefore multiple genes
Term
duplication
Definition
(a chromosomal mutation) a whole piece of a chromosome is duplicated
Term
deletion
Definition
(a chromosomal mutation) a segment of a chromosome goes missing
Term
inversion
Definition
(a chromosomal mutation) a segment of a chromosome flips upside down
Term
translocation
Definition
(a chromosomal mutation) a piece of a chromosome breaks off and reattaches onto another chromosome somewhere else
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