Shared Flashcard Set

Details

Test #1
Chapters 1-7
53
Microbiology
Undergraduate 2
02/05/2013

Additional Microbiology Flashcards

 


 

Cards

Term
Bacteria Characteristics
Definition
Prokaryotes, binary fission, usually friendly, crucial to the environment, peptidoglycan cell walls
Term
Archaea Characteristics
Definition
Prokaryotes, extreme environments (belly button), lack peptidoglycan
Term
Fungi Characteristics
Definition
Eukaryotes, chitin cell walls, multicellular (i.e. molds), unicellular (i.e. yeast), usually friendly
Term
Protozoa Characteristics
Definition
Eukaryotes, mobile via pseudopods, cilia, or flagella
Term
Algae Characteristics
Definition
Eukaryotes, cellulose cell walls, photosynthesis
Term
Virus Characteristics
Definition
Acellular, DNA and RNA core, only replicate inside a living host
Term
Taxonomy
Definition
Developed by Linnaeus, Genus species (italicized)
Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species
Dirty Kangaroos Play Chess On Fuzzy Green Squares
Term
pH
Definition
Base -OH, Acid -H, Salt disassociate to -H and -OH
Cells have a narrow range of tolerable pH
Term
Organic Molecules
Definition
Carbon skeleton and functional group
Term
Carbohydrates
Definition
Sugars & Starches, diverse functions (coding, structure, energy)
Term
Sugars
Definition
Monosaccharides-3 to 7 carbon atoms
Pentoses- i.e. deoxyribose
Hexoses i.e. glucose
Oligosacchrides- 2 too 20
Polysaccharides- tens or hundreds of monosacchrides joined together through dehydration synthesis
Term
Lipids
Definition
Can be simple C, H, O or can be complex C, H, O plus P, N, S
Term
Steroids
Definition
Part of membrane fluidity, four carbon rings with an -OH group
Term
Proteins
Definition
Essential to cell structure and function, enzymes, membrane transporters, flagella, toxins
Term
Amino Acids
Definition
Amino group, side group, carboxyl group,
Subunits or proteins, held together by peptide bonds, dehydration synthesis
Term
Protein Structure
Definition
Primary-amino acid sequence,
Secondary-pleats or helix,
Tertiary-more folding,
Quaternary-two or more polypeptides,
Term
Nucleotides
Definition
Pentose, phosphate group, base
Purine-double ring, Adenine and Guanine
Pyrimidine-single ring, Thymine, Uracil (RNA) and Cytosine
Term
DNA vs. RNA
Definition
DNA-Adenine pairs with Thymine, double stranded, messenger, ribosomal, transfer RNA
RNA-Adenine pairs with Uracil, single sanded, viral RNA
Term
Types of Light Microscopy
Definition
Compound Light, Darkfield, Phase-Contrast, Differential interference contrast, Fluorescence, Confocal
Cats Don't Play Darts In Flooded Clubs
Term
Compound Light Microscopy
Definition
Light Microscope
Used most commonly in our labs
Max resolving power of .2μm
Term
Phase Contrast Microscopy
Definition
Light MIcroscope
Good for viewing living organisms
Term
Fluorescence Microscopy
Definition
Light Microscopy
UV light
Can be used with fluorescent stains
Term
Confocal Microscopy
Definition
Light Microscopy
Very similar to fluorescence
Fine slices are examined and assembled into a 3D image by a computer, good for biofilm study
Term
Electron Microscopy
Definition
SEM-scanning, black and white, 3D
TEM-transmission, thin sections, 2D
Term
Probe Microscopy
Definition
STM (scanning tunnel) or AFM (atomic forces)
Uses lasers
Resolving power to visualize atoms
Term
Bacteria Shape: Coccus
Definition
diplococci (pairs), streptococci (chain of balls), tetrads (four balls), sarcinae (four balls on four balls), staphylococci (a bunch of balls)
Term
Bacteria Shape: Bacillus
Definition
SIngle rod
Diplobacilli (two rods)
Streptobacilli (lots of rods)
Coccobacillus (Rod ball mix, blob like)
Term
Bacteria Shape: Spiral
Definition
Vibrios (curved rod, boomerang)
Spirilla (helical and rigid)
Spirochete (helical and flexible)
Term
Glycocalyx
Definition
AKA capsule, slime layer, EPS
Protection from macrophages
Allows adherence
Term
Flagella
Definition
Motility (propeller), may also used for adhesion or secretion
Vary between G+, G-, and Euks
Term
Flagella Structures
Definition
Monotrichous (one on one side)
Amphi (One on each side)
Lopho (multiple on one side or both sides)
Peri (all over)
Term
Axial Filaments
Definition
Unique to spiral shaped bacteria, built into the membrane, rotation of filaments cause the bacteria to rotate causing movement
Term
Fimbriae or Pili
Definition
short, thin, fibrous
help with adhesion. minor role in motility
sex pili are used for conjugation (transfer of plasmid)
Term
Peptidoglycan
Definition
composed of disaccharides, crosslinked together
amount determines if cell is gram positive or gram negative
Term
Active Transport
Definition
Necessary for certain ions and achieving high concentrations
Term
Osmotic Pressure
Definition
Isotonic (equal), Hypotonic (lower on outside), Hypertonic (higher on outside)
Term
Ribosomes
Definition
Protein synthesis in both proks and euks
Term
Inclusions
Definition
reserve deposits of macromolecules for later use, can be used for diagnostics and identification
Term
Endospores
Definition
resting structures formed by some bacteria when in a inhospitable environment
Term
Gram Staining
Definition
Heat, Crystal Violet, Gram's iodine, ethanol, safranin
Positive Purple
Negative Pink
Term
Endocytosis
Definition
Engulfing large particles
phagocytosis-particles
pinocyotosis-fluid
Term
Enzymes
Definition
-ase
lower activation energy, catalysts which speed up reaction
Bind to substrates at the active site
Term
Inhibitors
Definition
Competitive-block active site
Alosteric-attach at another site besides the active site
Term
Electron Transport Chain
Definition
Glycolysis, Krebs Cycle, ETC (oxidize NADH and FADH2)
Term
Fermentation
Definition
Another way to breakdown organic compounds for energy, glycolysis and pyruvic cycle, no oxygen necessary
Term
Quorum Sensing
Definition
how bacteria with biofilms communicate
Term
Chemotrophs
Definition
hetero-use organic compounds
auto-use CO2 as a carbon source and derive energy from inorganic compounds
Term
Requirements for Bacterial Growth
Definition
Physical-Temp, pH, osmotic pressure
Chemical- CNSP, trace elements, organic growth fctors (vitamins), oxygen
Term
Reactive Oxygen Species
Definition
Singlet oxygen, superoxide free radicals, hydrogen peroxide, hydroxyl radical, ozone
Term
Biofilms
Definition
adhesion, submerged in liquid, surrounded by EPS, quorum sensing
Term
Biofilm
Definition
Adhesion, Growth, Dispersal
Gene regulation is different
Illness may not respond to antibiotics and may be culture negative
Term
Types of Culture Media
Definition
Differential-visual differentiation
Selective-encourages the growth a a target organism
Streaking to isolate individual colonies
Term
Growth of Bacteria
Definition
Lag, Log, Stationary, Death
Supporting users have an ad free experience!