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Details Title Summary of Test Performed Description Descriptions of Lab Test Total Cards 45 Subject Microbiology Created 11/16/2011

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Term	Definition NEGATIVE. Fast fermenters turn from dark green to black. Slow fermenters turn are pink/maroon.
Term	Definition Positive. No growth white/colorless
Term EMB	Definition Uses Esosin Methylene blue media.It is select for gram NEGATIVE BACTERIA and DIFFERENTIAL for lactose fermenters.
Term	Definition Growth is gram NEGATIVE (fermenter) salmon color. No growth is gram POSITIVE (non-fermenters) blue-bluegreen.
Term HE	Definition Uses Hektoen enteric agar media. Selective for gram NEGATIVE BACTERIA DIFFERENTIAL for lactose fermenters
Term	Definition Growth gram POSITIVE. No growth gram NEGATIVE.
Term PEA	Definition Uses Phenylethyl alcohol agar media. Select for Staphylococcus DIFFERENTIAL for mannitol fermenters.
Term	Definition Growth is gram POSITIVE (fermenter) YELLOW. No Growth is gram NEGATIVE (non-fermenter) RED.
Term MS	Definition Uses Mannitol salt agar media and phenol red reagent. Select for Staphylococcus DIFFERENTIAL for mannitol fermenters.
Term	Definition No clear zone has NEGATIVE results. Clear zone around bacterial growth has POSITIVE results.
Term Caseinase Production	Definition Uses Milk agar media. It also detects if bacteria can break down casein (C.violaceum).
Term	Definition Gram POSITIVE as evidenced by the liquidation of the media.
Term	Definition Gram NEGATIVE as evidenced by the solidity of the media.
Term Gelatinase Production	Definition Uses Nutrient gelatin and can detect if bacteria can break down gelatin (S.aureus, L. monocytogenes).
Term	Definition Salmonella typhimurium left is negative no clear zone. Pseudomonas aeruginosa right is positive clear zone surrounding the growth.
Term Lipase Production	Definition Uses Tributyrin agar media and detects if bacteria can break down tributyrin.
Term	Definition Purple color after iodine added NEGATIVE results. Clear zone around bacterial growth when iodine is added POSITIVE results.
Term Amylase Production	Definition Uses Starch agar media and iodine reagent. It detects if bacteria can break down carbs (C. diphtheriae, C. perfringes, B. anthracis).
Term	Definition Red is NEGATIVE. Yellow (with full small tube inside) POSITVE. Yellow (with tube slightly not full inside) Fermentation and gas have been produced.
Term Carb Fermentation	Definition Uses Durham tubes, Fermentation broth=carb (glucose,lactose,sucrose). It uses Phenol reb reagent and detects if bacteria can ferment different carbs.
Term	Definition RED after adding reagents A&B POSITIVE. If added zinc COLORLESS POSITIVE. Remained RED after adding zinc NEGATIVE.
Term Nitrate test	Definition Uses Nitrate reagent A&B and Zinc. It identifies the way nitrate is reduced in certain bacteria.
Term	Definition Colorless NEGATIVE. PURPLE POSITIVE.
Term Oxidase Test	Definition Media/Reagent TMPD. It detects cytochrome c oxidase activity (All Enterobacteriacease are NEGATIVE).
Term	Definition No bubbling POSITIVE. Bubbling NEGATIVE.
Term Catalase Test	Definition Uses Hydrogen peroxide reagent. Test for Catalase Production.
Term	Definition NEGATIVE YELLOW. POSITIVE RED.
Term Indole Test	Definition Uses Tryptone broth and Kovac’s reagent. Test for ability to break down Tryptophan and yield indole.
Term	Definition RED POSITIVE. YELLOW NEGATIVE.
Term Methyl Red Test	Definition Uses MR-VP broth and Methyl Red reagent. It has the ability to ferment glucose and form acids.
Term	Definition No color change NEGATIVE. Deep PINK POSITIVE.
Term Voges-Proskauuer Test	Definition Uses MR-VP broth and Barritt’s reagent. It detects presence of acetoin.
Term	Definition Turns from green to royal blue POSITIVE.
Term	Definition No color change, remains green NEGATIVE.
Term Citrate Test	Definition Uses Simmon’s citrate agar media and bromthymol blue reagent. It detects the ability to utilize citrate acid as sole source of carbon.
Term	Definition dark green POSITIVE.
Term	Definition No color change NEGATIVE.
Term Phenylalanine Deamination	Definition Uses Phenylalanine slantsand 10% ferric chloride. It test for Phenylalanine Deamination – most bacter that produce this are pathogenic (ie Proteus, Morganella).
Term	Definition PURPLE POSITIVE
Term	Definition No color change YELLOW NEGATIVE.
Term Lysine/Ornithine Decarboxylation Test	Definition Uses Lysine and Ornithine broth, mineral oil, Bromcresol purple. Test for lysine and ornithine decarboxylase (one way to overcome IMViC tests limit).
Term	Definition No color change is NEGATIVE. Black precipitate POSITIVE.
Term Hydrogen Sulfide Test	Definition Uses Peptone iron agar media and deep containing ferrous sulfate. It detects hydrogen sulfide gas production.
Term	Definition Orange and yellow are NEGATIVE. Pink is POSITIVE.
Term Urease Test	Definition Uses Urea broth and phenol red. It detects urease production and is useful in distinguishing Proteus.

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