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Specimin collection Skills Test 4
Specimen collection skills test 4
66
Nursing
Undergraduate 3
04/07/2014

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Term
Blood sample
Definition
Glucose, cultures
Term
Urine sample
Definition
Routine, mid-stream or clean catch
Term
Nursing Responsibilities
Definition
Provide comfort privacy and safety. Patient prep, follow OSHA standards, reporting results to primary care provider. Labeling, storage, and transportation
Term
What precautions are used for collection
Definition
Sterile technique usually and standard precautions
Term
2011 national patient safety goal number 2
Definition
Includes reporting of critical test/diagnostic procedures on a timely procedure
Term
Type 1 diabetes or "Insulin dependent diabetes"
Definition
-Beta cells of pancreas no longer produce insulin
-Healthy diet and physical activity important
-Usually diagnosed in children/YA
-Accounts for 10% of diabetes
-Previously called IDDM or insulin dependent diabetes mellitus
-ALWAYS requires insulin
Term
Type II diabetes
Definition
-Beta cells continue to make insulin BUT the body cells are RESISTANT to insulin produced
-Adult onset diabetes
-Can develop at any age
-Overweight/inactive increases chances
-90% of diabetes
-Previously called NIDDM or non-insulin dependent diabetes mellitus
-Treated with oral hypoglycemic, insulin, or a combo of both, physical activity
Term
Gestational Diabetes
Definition
developed in late pregnancy, usually goes away, but more likely to develop type II later in life
Term
Capillary Blood Glucose
Definition
Measures Blood glucose levels. Less painful than venipuncture. Glucometers vary.
Term
What to remember with BG tests
Definition
-determine if it is to be fasting, before meals, after meals, ect
-Note if insulin is scheduled, prn or per carbs
-Review med hx of anticoagulants
-Look for hypo/hyperglycemia S/S
Term
When are glucose values higher?
Definition
1-3 hours after ingesting a meal
Term
Hgb A1c normal levels
Definition
4%-5.6% in non-diabetics
Term
Hgb A1c pre-diabetic (have a higher chance of getting diabetes) levels
Definition
5.7-6.4%
Term
Hgb A1c diabetic levels
Definition
6.5% and up
Term
Hgb A1c diabetic goal level
Definition
Less than 7%
Term
Neonate/Infants up to age 2 BG site
Definition
Heel. Never middle of heel. Put a warm pack on prior to the stick.
Term
Site for BG for adults/children over age 2
Definition
Side of fingertip
Term
Site for patients with hypovolemia or extremity edema
Definition
Earlobe
Term
Children considerations for BG test
Definition
Allow children to choose which finger to use, reassure child that procedure is not a punishment
Term
GB monitoring: first drop of blood
Definition
Wipe it off with gauze because it has serous fluid that might effect the results
Term
Blood Tests Sites
Definition
Diagnostic aids. CBC, lipid profile. Can be obtained via skin puncture, venipuncture, arterial puncture.
Term
What is the purpose of blood culture
Definition
Detect bacteria in blood, require 2 specimens from two different sites. May require an anaerobic and aerobic vial for each site.
Term
Do you obtain a culture before or after antibiotics are started?
Definition
Before. BLOOD IS ALWAYS STERILE TECHNIQUE
Term
What does it mean if both cultures come back positive? one positive and one negative?
Definition
Both positive is considered a bacterial infection. One positive is considered a contaminated specimen
Term
Urine Specimen Routine
Definition
Routine that can be collected at any time. No specific guidelines. Important to avoid contaminants as much as possible. Instruct patient not to touch cup inside or cup lid
Term
Mid stream or clean catch
Definition
Preferred. 1st cleanse urethral site, voids small urine stream, stops, then catches urine in sterile container. Culture and sensitivity. Can get any time during day or night
Term
Timed Urine specimen
Definition
Specified time frame where we collect ALL urine. Bladder is emptied prior to test. All urine is collected and final urine is collected at the end (usually 24 hours). Usually refrigerated during time. Important to be accurate.
Term
What is a 24 hour urine catch (timed urine) used for?
Definition
Used to determine levels of specific components such as protein/creatinine
Term
Infants consideration
Definition
Use a specimen bag that has adhesive backing that attaches to them
Term
Children considerations
Definition
Explain in child terms. If possible, have parent assist
Term
Older adult considerations
Definition
May have difficulty controlling urine stream. Physical limitations may interfere with collection
Term
Lab values: Urine pH normal
Definition
4.6-8.0
Term
Lab values: Urine specific Gravity normal
Definition
1.010-1.025
Term
Lab values: protein, glucose, ketones, blood normal
Definition
all should be negative
Term
Stool collection purpose
Definition
Analyze GI function.
Term
Stool Collection purposes
Definition
1. too much fat in GI. too much bile can indicate liver problems. We do this to check levels.
2. Check for bacterial and viral infections
3. check for ova and parasites (Giardia),
4. bleeding in GI tract.
Term
How many samples for stools are usually required?
Definition
Three. Especially for presence of ova and parasites.
Term
Most common stool analysis
Definition
Fecal Occult test
Term
How do we prevent stool sample contamination?
Definition
Have patient void before specimen
Term
How do we transfer stool to container?
Definition
1-2 tongue blades are used
Term
What are the requirements for a formed stool sample?
Definition
1 inch
Term
What are the requirements for a liquid stool sample?
Definition
15-30cc for liquid
Term
How do we transport stool samples?
Definition
In a biohazard bag.
Term
Does stool need to be sterile?
Definition
No, it does not.
Term
Occult Blood Stool collection: Hemoccult test
Definition
Needs 3 separate, consecutive stool samples (may take several days--pt can do this at home as well)
Term
EZ detect or Colocare
Definition
Flushable reagent pads, no handling of sample, easy at home. Basically putting the pad in the toilet and watching for a blue or green color change which indicates blood in stool. 2 min for results.
Term
Hemoccult considerations
Definition
1. DO not collect during menses (wait 3 days after at least)
2. Do not collect with actively bleeding hemorrhoids
3. If there is blood in the urine
Term
Hemoccult Diet
Definition
3 days before testing. Avoid:
1. Vitamin C (more than 250mg) can cause false negative!
2. Red Meat
3. Iron tablets
4. Aspirin, NSAIDS, anticoagulants
5.Radishes, turnips, horseradish, melons, beats.
Term
Guaiac positive (stool sample hemoccult) (blood positive)
Definition
positive has a blue surrounding. Negative does not.
Term
Hemoccult considerations
Definition
Screening only. Do not rule out. Can have false positives and negatives.
Term
False Positives of Hemoccult
Definition
Ulcers, fissures, hemorrhoids, benign polyps, diverticulitis, constipation
Term
False Negatives of hemoccult
Definition
Polyps and cancer bleed intermittently. Misses about 60 percent of polyps and about 40-50 percent of cancer
Term
Nose and throat specimens
Definition
Nose: collected thru nasal passages
Throat: collected thru mucosa of oropharynx and tonsillar regions. Used to determine bacterial or viral infections including strep, whooping cough, and some meningitis, flu, RSV, ect
Term
Patient teaching nose/ nasalpharynx collection
Definition
Blow nose first before nose collection. May feel like sneezing.

Wire: put in 3-4 inches. Do not press hard. Rotate or held in place for 10 seconds.
Q-Tip thing: gently rub along the septum and nasal floor to collect
Term
Patient teaching throat collection
Definition
Open mouth, tilt head back, may feel like gagging. Use tongue depressor, do not touch any part of tongue or side of mouth. Touch tonsils, do not touch any part of mouth
Term
Sputum collection purpose
Definition
Incubate expectorated material to identify respiratory bacterial pathogens. Sputum is thick!
Term
Do healthy individuals have sputum?
Definition
No! only saliva
Term
Sputum collection tells us
Definition
If it is bronchitis, pneumonia, or TB
Term
Types of specimens
Definition
Cytology/Culture and sensitivity: used to tell us what antibiotic to use
Acid Fast Bacilli: used to see if pt has TB
Term
Cytology
Definition
Used to see if cancer is in the lungs and the specific cell type
Term
Obtaining a sputum specimen
Definition
Expectorated, induced coughing, bronchoscopy, suctioning with trap
Term
Acid Fast Bacilli
Definition
3 consecutive MORNING sputum collections that need to be at the lab within 1-2 hours
*Negative cultures do NOT necessarily mean that the pt does not have TB.
Term
Assessment prior to sputum collection
Definition
Type of collection required, RS status, Assess when pt last ingested food, when they last had surgery, ect
Term
Sputum collection guidelines: How much do you need?
Definition
1-2 tsp or 5-10cc of sputum
Term
What position should the pt be in for sputum collection?
Definition
Sitting up in fowlers
Term
What do you document for sputum?
Definition
Color, consistency, odor, volume, viscosity, presence of blood
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