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Section Exam #3
Notecards for thrid test
65
Biology
11th Grade
01/09/2010

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Term
How does Mitosis counterbalance fertilization?
Definition
Mitosis splits one cell into 4 haploid cells and fertilization puts two haploid cells together to make one diploid cell.
Term
________ chromosomes line pair up during prophase 1 of meiosis. They form a _______.
Definition
Homologous, Tetrad
Term
What is the purpose of mitosis 1 in meiosis?
Definition
To separate homologous chromosomes.
Term
The first mitosis of meiosis results with 2 __________ (haploid/diploid) cells.
Definition
Haploid
Term
What is the purpose of mitosis 2 in meiosis? What is being split and what are they being split into?
Definition
To separate sister chromatids. Diads, Monads
Term
The second mitotic division of meiosis results in _____ cells that are _________.
Definition
4, haploid
Term
What are the results of one cell going through spermatogenesis?
Definition
4 haploid sperm cells
Term
What are the results of one cell going through Oogenesis? What is different about these divisions?
Definition
1 haploid Ootid, and 3 polar bodies (that will die), the Ootid will recieve most of the cytoplasm from the mother cell
Term
What were the 4 principles that Mendel came up with and what are they?
Definition
1. Each parent contributes 1 gene for a trait, inherit 2 genes per trait
2. Dominance and Recessiveness - some traits are more dominant than others and the recessive ones will be hidden
3. Law of Segregation - copies of the gene separate so that each gamete only receives one copy
4. Independent Assortment - alleles of different genes assort independently of one another during gamete formation
Term
What are purines?
Definition
Double Ringed, Adenine and Guanine
Term
What are pyrimidines?
Definition
Single Ringed, Cytosine and Thymine
Term
Opposites strands of DNA are ___________.
Definition
Antiparallel
Term
What is the name of the enzyme that straightens sections of the DNA before replication?
Definition
Topoisomerase
Term
What is the name of the enzyme that "unzips" the DNA by breaking the hydrogen bonds? What comes after to keep the two strands apart?
Definition
Helicase, Single Stranded Binding Protein
Term
When the free nucleotides come in, during DNA replication, they are attached by what?
Definition
DNA Polymerase
Term
What is the purpose of RNA primer?
Definition
It helps to build the new DNA strand.
Term
What direction are new nucleotides inserted in DNA replication?
Definition
5' to 3' in the new strand that is being made
Term
What enzyme links the leading and lagging strands? What is another name for these strands?
Definition
DNA Ligase, Okasaki fragments
Term
What enzyme goes through the DNA after replication to make sure there are no mistakes?
Definition
DNA Repair Enzyme
Term
What are 3 main differences between RNA and DNA?
Definition
DNA is double stranded, RNA is single. DNA uses doxyribose sugar, RNA uses ribose sugar. DNA has thymine, RNA has uracil.
Term
During transcription what enzyme straightens the DNA? Breaks the hydrogen bonds? Holds sides apart?
Definition
Topoisomerase, Helicase, Single Stranded Binding Protein
Term
What enzyme brings in new nucleotides during transcription to make the mRNA? What direction are the made in the new strand?
Definition
RNA Polymerase, 5' to 3' (goes up the left side, down the right)
Term
Where does the RNA polymerase attach to during transcription?
Definition
Promoter
Term
What attaches to the broken off mRNA during transcription?
Definition
GTP and Poly-A-Tail
Term
What enzyme "glues" the DNA back together after the introns are spliced out of it?
Definition
RNA Ligase
Term
What enzyme cut the introns from the mRNA? What is the name of the self splicing RNA?
Definition
splicosomes, ribozymes
Term
What is the start codon?
Definition
AUG
Term
What codon do you use to look up what amino acid it is?
Definition
the mRNA codon
Term
What does transfer RNA find? What is done with the anticodon?
Definition
The anticodon, the correct amino acid is made
Term
What are the three different sites of the large subunit of the ribosomal RNA?
Definition
Exit Site, Peptide Site, Amino Site
Term
What are the newly formed amino acids connected by during translation?
Definition
Peptide Bonds
Term
What is worse in translation? Substitution or addition/deletion?
Definition
Addition/Deletion
Term
What type of organism does the operon model include?
Definition
Bacteria
Term
What is the structure of an operon?
Definition
Regulator, Promoter, Operator, Structural Genes. Regulator doesn't have to be next to the promoter/operator
Term
What is binded to the promoter in an operon?
Definition
RNA Polymerase
Term
What has to be attached to the operator of an operon for it to be "off"?
Definition
a repressor molecule
Term
What is the effect of a represser molecule on the RNA polymerase in an operon?
Definition
Makes it so the polymerase can't attach or can't move.
Term
What codes for the represser molecule in an operon?
Definition
Regulator
Term
What makes a represser molecule go away in an operon?
Definition
An effector molecule. It changes the shape of the represser molecule.
Term
What is polyccistronic mRNA?
Definition
polycistronic mRNA carries the information of several genes, which are translated into several proteins.
Term
What are the two types of control for bacteria transcription/translation?
Definition
Inducilbe - Makes transcription happen by presence of the substrate causing the cells to make the necessary enzyme.
Repressible - the product acts as the effector molecule shutting down transcription of those genes.
Term
What is a plasmid? What is it called if it is in the DNA loop temporarily?
Definition
An extra loop of DNA that is optional and very small. Episome.
Term
What are the two parts of viruses?
Definition
protein capsid and DNA or RNA
Term
What is a RNA virus called?
Definition
Retrovirus
Term
What process do retroviruses do to get into the host's DNA? What enzyme is used?
Definition
Reverse Transcription. Reverse Transcriptase.
Term
What are the two reproductive cycles of viruses? What are the steps? What is the difference between the two?
Definition
Lytic Cycle and Lysogenic Cycle.
1. Attachment
2. Entry (insert genetic material)
3. Replicate
4. Assembly
5. Release
In the lysogenic cycle, there is a pause after entry.
Term
What is it called when viral DNA is fused with bacterial DNA? In eukaryote DNA?
Definition
Prophage, Provirus
Term
What is something called that prevents a virus from replicating?
Definition
Interferon
Term
What is transduction?
Definition
When a virus spreads its genes to the host.
Term
What is a Lambda phage?
Definition
When a virus deletes the gene that causes the cell to lyse.
Term
What cuts DNA and what are the two types of cuts?
Definition
Restriction Enzymes. Clean cuts and staggered cuts
Term
What are the ends of staggered cuts called?
Definition
COS Regions (sticky ends)
Term
What is a transposon? What is a comlex transposon? What is the enzyme for this?
Definition
A "jumping gene". Moves spots on the DNA loop. When two genes jump taking the genes inbetween them as well. Transposase
Term
What are the 3 forms of DNA packing?
Definition
B DNA- most common, tightly coiled right handed helix. A DNA- right handed helix, not as tight. Z DNA- is zigzagged, left handed helix.
Term
When is the DNA least compacted?
Definition
In chromatin form.
Term
What is the structure of DNA called when histones (H1, H2A, H2B, H3, H40 clump around a section of the DNA?
Definition
Nucleosome
Term
What is the structure of DNA when nucleosomes are folded up?
Definition
30nm fibers
Term
When is the structure of DNA the most condensed DNA can be?
Definition
Metaphase Chromosomes
Term
What is Looped Domain?
Definition
When 30nm fibers are folded up more and attached to a protein scaffold
Term
What is a heterochromatin? What is a euchromatin?
Definition

Heterpchromatin- Stains well, genes off, condensed, twisted.

Euchromatin- loose, relaxed, genes are on, stain weakly.

Term
What is there always on DNA that is in heterochromatin form?
Definition
Centromere
Term
What are Barr Bodies?
Definition
Extra X chromosomes that are always kept in heterochromatin form.
Term
What are the 4 ways to control gene transcription in eukaryote cells?
Definition
1. Condensing DNA.
2. Methylation- adding CH3 to cytosine
3. Histone Acetylation- adding acetyl groups tohistones causing DNA to not be bound as tightly turning transcription on.
4. Use of effector molecule, promoter, regulator, operator.
Term
What are cancer genes called?
Definition
Oncogenes
Term
What is another name for a carcinogen?
Definition
Mutagen
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