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Scanning Electron Microscopy
Scanning Electron Microscopy
60
Biology
Undergraduate 4
02/05/2013

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Cards

Term
Euclid
Definition

Ray - path of the light wave

Cone of light is focused by a lens on the object

Term
Reflection
Definition
the angle of incidence equals the angle of reflection
Term

Ibn Sahl 

Wellebrord Snellius

Definition

Law of Refraction

n1 sin(theta1) = n2 sin(theta2)

Term
Christian Huygens
Definition

Huygens principle states that a wavefront can be thought of as an infinite number of hemispherical wavefronts

allowed scientists to understand particle-wave duality of light

Term
Light microscope
Definition

invented after 1600

followed the invention of the telescope in 1608

no reliable date of invention, given to prominent people

Hans Maartens

Term
Ernst Ruska 
Definition

Developed the early electron microscope

Invented osciolloscope to prevent power surges

used oscilloscope to create the electron microscope

Term
Abbe equation
Definition

equation for resolution

cannot see details smaller than wavelength of light

detail resolved in sample  is about half the wavelength at best

Term
focal point
Definition

the point at which light (parallel rays) converges after passing through a biconvex lens 

fixed in light microscopes

variable in electromagnetic microscopes

Term
front space
Definition
where the object is placed (where the rays are parallel) (-u)
Term
back space
Definition
where rays are converged 
Term
focal length
Definition
the distance to the focal point (in the back space) (+v)
Term
geometric optics
Definition

ray tracing: rays arriving on axis go parallel to their direction in the front space

all other rays are refracted, converged in biconvex lens

Term
lens equations
Definition

1/v-1/(-u)=1/f becomes 1/v+1/u=1/f

M (magnification) = v/u

if u=v=2f, then M = 1x

Term
SEM Lens equations
Definition

1. In SEM, the lenses only exist to focus the beam from the source

2. resolution depends on spot size. you don't want to magnify the image of the source very much

3. magnification relationship not too far from reality

 

Term
Oersted
Definition
showed that a flowing current generates a magnetic field (lead to electromagnetic lens)
Term
Solenoid
Definition
spiral turn of wire, at the heart of the electromagnetic lens
Term
geometric optics in electrons
Definition

1. electron spirals through the lens

2. no change in velocity as in the light lens

3. same lens equations hold true

Term
Difference between SEM and TEM
Definition
no objective lens in SEM
Term
working distance
Definition
the distance of the object from the objective lens
Term
depth of field vs working distance
Definition
shorter working distance = smaller depth of field
Term
Major difference between light and electrons
Definition
wavelength and frequency
Term
voltage vs wavelength of electron
Definition
higher voltage = shorter wavelength
Term
basic equation for resolution
Definition
d = 0.61λ/nsinα
Term
equation for numerical aperture
Definition
nsinα
Term
resolution (definition)
Definition
the smallest distance between details you can see in the image
Term
does NA remain the same regardless of where one is in the raster pattern?
Definition
No, changes with the raster pattern
Term
how does aperture effect gathering power and resolution?
Definition

large spot = better gathering power, low resolution

small spot = poor gathering power, high resolution

Term
diffraction
Definition

occurs whenever waves interact with matter

huygens' principle

Term
electron refraction
Definition

lowest in polepiece lens

intermediate in shrouded lens

greatest in simple solenoid

Term
Why is the polepiece lens used?
Definition

electrons spent too much time in lens

coated lens in iron with brass piece

entire field emitted through brass piece, making smaller, thinner lens (better)

condensed electron field thin as pancake, close to ideal lens

Term
spherical abberation
Definition

rays far from axis converge close to the lens

rays near axis converge further from lens

creates a circle of minimum confusion instead of a focal point

Term
chromatic abberation
Definition

different wavelengths of light converge differently

blue focuses closer, red is further away

opposite in electron microscope

Term
astigmatism
Definition
rays converge asymmetrically, refraction stronger in one direction than another
Term
how is astigmatism caused in electron microcopes?
Definition
volatiles from sample and oils from pump insulate parts of the lens and magnetic field
Term
how is astigmatism corrected in electron microcopes?
Definition

stigmator

charged poles pull the beam on one direction or another, can change the direction and magnitude of the pull

Term
airy's disk with interference fringes
Definition
d = contains half the beam current
Term
scattering (two types)
Definition

elastic - bouncing off protons

inelastic - knocks electrons out of electron shell

Term
Hydrogen irradiance
Definition

IR, visible, UV

fluorescence

electron orbitals

Term
electron orbitals
Definition

differ in terms of energy

n = quantum number of electron shell

increasing energy with increasing n

E = nhf

Term
inelastic scattering
Definition
incoming electron knocks out electron from an atom, electron fills from another quantum shell, releasing x-radiation
Term
Bohr theory of atomic structure
Definition

electrons move around nucleus in energy levels

more energy at higher levels

equation for angular momentum

explains frequency of hydrogen irradiance spectrum

n=/=0

couldn't solve anything other than hydrogen

Term
Pauli exclusion principle
Definition

No 2 electron orbitals can have the same quantum signature

azimuthal, spin

Term
Heisenberg uncertainty principle
Definition
cannot measure the state of 2 electrons (one disturbs the other)
Term
voltage vs interactions
Definition

increasing voltage increases interactions (zone of interaction)

low voltage = minimal elastic and inelastic scattering

Term
energy of emitted electrons
Definition
determined by atomic structure
Term
mean free path
Definition

how far the electron moves without colliding (elastic)

shorter for larger atoms, reason for coating

Term
Peak III (from e- emission graph)
Definition
secondary electrons, kept from inventing a usable electron microscope
Term
how are electrons captured?
Definition

positive field

electrons are "lazy" and change trajectory easily

boost +10kV before scintillator, to keep from getting stuck

scintillator made of scintillin produces photons when electrons strike

Term
scintillator
Definition

made of scintillin

beta emmitter

converts electrons into photons

some electrons lost on the sides of the scintillator

Term
describe the path of the signal through the secondary signal detector?
Definition
collimator and high voltage lead (aluminum) attracts electrons > scintillator (electrons>photons) > light pipe (quartz or lucite) > photocathode (converts photons>photoelectrons) > photomultiplier tube (PMT; amplifies photoelectrons into a usable signal, gain increased at each dynode) > CRT
Term
photocathode
Definition
after light tube, converts photons from scintillator into photoelectrons
Term
PMT
Definition

photomultiplier tube

amplifies the signal at each dynode and sends it to the CRT

Term
Everhart Thornley Detector
Definition
The most common design for a secondary detector
Term
backscatter detector
Definition
usually doughnut shape under the final lens, can be in the same port as the secondary detector
Term
Why two different detectors? (graph)
Definition

Two different types of scattering

Emission energy as a fraction of beam energy

Region I. backscatter, Region II. backscatter with energy loss, Region III. secondary emission

Term
backscatter efficiency vs atomic number
Definition
high atomic number means high backscatter
Term
mean path property (mean free path)
Definition

electron beam will go deep into sample with low density and atomic weight

can go a long distance without colliding

Term
z contrast (atomic number contrast)
Definition
if z is high then high brighness
Term
signal noise relationship in the secondary detector
Definition

signal bounces off specimen (100 electrons)

scintillator boosts signal strength

60-70 quanta per 100 quanta

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