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Ryder-Gen Bio(2) 131-112
Test #1
11
Biology
Undergraduate 2
02/24/2011

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Cards

Term
Purpose of the three controls in griffiths "transformation" experiment
Definition
Term
Results and conclusion of Griffiths "transformation" experiment 
Definition
he killed pathogenic material with heat and then mixed the cell remains with living bacteria of the nonpathogenic strain, some of the living cells became pathogetic. In other words R bacteria transformed into pathogenic S bacteria by an unknown, heritable substance from S cell allowed R cells to make capsuls
Term
Avery's question,experiment, results, conclusion
Definition

Q: what is Griffiths 'transforming' substance: RNA-DNA-Protein

Experiment: extracted cellular contents of the heat-killed pathogenic bacteria. used specific treatments that inactivated each of the three types of molecules. tested to see which one transformed and which one didnt. 

Results: when DNA was allowed to remain active, transformation occured

Conclusion: DNA is the genetic material

Term
Structure of DNA by data from Watson and Crick
Definition

x-ray diffraction: fiber of DNA, x-rays ar diffracted by atoms of DNA molecules. helped them figure out that it had a helical shape with two strands present.  center = low resolution info outer = high resolution info

Chargaffs Rule: A=T G=C

 

Term
Semi-conservative replication
Definition

-The DNA strand splits and replicates resulting in 2 identicle molecules (each with strand of old molecule with template for new molecule)

*2 chromatis *occurs in Interphase, S phase (synthesis)

Term
how is genetic information stored in DNA and define allele
Definition

Allele: a version of a gene that had a unique sequence of bases

 DNA-(trasncription)-RNA-(translation)-Protein

Term
Illustrate gene expression in bacteria and eukaryotes
Definition
in notes and in book pg. 329
Term
What is a gene and a codon
Definition

- A discrete unit of hereditary information cosisting of a specifit nucleotide sequence in DNA or RNA

-3 base unit

Term
What are the modifications of the eukaryotic cells for pre-mRNA
Definition

1) initiation

2) elongation

3) termination

Term
Phases of Transcription
Definition

1) initiation: after an RNA polymerase binds to the promoter, the DNA strabds unwind, and the polymerase initiates RNA synthesis at the start point on the template strand.

2) Elongation: the polymerase moves downstream, unwinding the DNA and elongating the RNA transcription 5'-3'. The DNA strands re-form a double helix.

3) Termination: the RNA transcrip is released and the polymerase detateches from the DNA

pg. 332

Term
molecular component of translation and what are their functions.
Definition

1) mRNA: info to produce protein

2) tRNA: bonds amino acids & transferes it down into the ribosome

-anticodon: a nucleotide triplet at one end of a tRNA molecule that recognize a particular complemetary codon on an mRNA molecule.

3) Amino Acid: an organic molecule prossesing both carboxyl and amino groups. amino acids serve as monomers of polypeptides

-aminoacyl-tRNA synthetase: an enzyme that joins each amino acid to the appropriete tRNA

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