Shared Flashcard Set

Details

Respiration, Translation and Transcription
Respiration, Translation and Transcription
74
Biology
Undergraduate 2
02/17/2011

Additional Biology Flashcards

 


 

Cards

Term
Adenosine triphosphate (ATP)
Definition
Has high potential energy and allows cells to do work. As cells process sugar, the energy that is released is used to transfer a phosphate group to adenosine diphosphate (ADP)
Term
Exergonic reactions
Definition
reactions that release energy
Term
Endergonic Reactions
Definition
Reactions that require energy
Term
Phosphorylation
Definition
The addition of a phosphate group to a substrate; phosphorylation of proteins is exergonic because the electrons in ADP and the phosphate group have much less potential energy than they did in ATP.
Term
Reduction-oxidation reactions
Definition
a class of chemical reactions that involve the loss or gain of one or more electrons; they drive the formation of ATP.
Term
Nicotinamide adenine dinuleotide (NAD+)
Definition
Reduced from NADH which readily donates electrons to other molecules and is said to have "reducing power."
Term
Glycolysis
Definition

One molecuel fo glucose is broken into two molecules of the three-carbon compound pyruvate.

 

2 ATP molecules are produced from ADP and 1 molecule of NAD+ is reduced to form NADH.

Term
Pyruvate processing
Definition

Pyruvate is processed to form the compound acetyl-CoA.

 

During this step of respiration, another molecule fo NADH is produced.

Term
Citric acid cycle (KREBS cycle)
Definition

Acetyl-CoA is oxidized to two molecules of CO2. 

 

During this sequence of reactions, more ATP and NADH are produced and FAD is reduced to form another electron carrier, FADH2.

Term
Electron Transport 
Definition

Electrons from NADH and FADH2 move through a series of proteins called an electron transport chain (ETC).

 

The potential energy released during these redox reactions is used to create a proton gradient across a membrane; the ensuing flow of protons back across the membrane is used to make ATP.

Term
Cellular Respiration
Definition
Any suite of reactions that produces ATP in an electron transport chain.
Term
Feedback inhibition
Definition

when an enzyme in a pathway is inhibited by the product of the reaction sequence.

 

The product molecule "feeds back" to stop the reaction sequence when the product is abundant.

Term
cristae
Definition
Layers of sac-like structures within the interior of th mitochondrion
Term
Mitochondrial matrix
Definition
The region inside the inner membrane but outside the cristae.
Term
Coenzyme A (CoA)
Definition

Acts as a coenzyme by accepteing and then trasnferring an acetyl group (-COCH3) to a substrate.

 

Pyruvate reacts with CoA, through a series of steps, to produce acetyl CoA, which is later oxidized to CO2.

Term
Electron Transport Chain (ETC)
Definition

As electrons are passed from one protein to another in the chain, the energy released by the redox reactions is used to pump protons across the inner membrane of mitochondria.

 

After this proton gradient is established, a stream of protons through the enzyme ATP synthase makes part of the protein spin, driving the production of ATP from ADP and P_i. 

Term
Fermentation
Definition

A metabolic pathway that regenerates NAD+ from stockpiles of NADH; it allows gylcolysis to continue producing ATP in the absence of the electron acceptor required by the ETC.

 

Allows AT to be produced int eh absence of oxygen. In organisms that usually use oxygen as an electron acceptor, fermentation is an alternative mode of energy production when oxygen supplies temporarily run out.

Term
Genome
Definition
a cell's entire complement of DNA
Term
DNA Polymerase
Definition
An enzyme that polymerizes deoxyribonucleotides to DNA; catalyzes DNA synthesis.
Term
deoxyribonucleoside triphosphates (dNTPs)
Definition
monomers that act as substrates in polymerization reactions. The N in dNTP stands for any of the four bases found in DNA: adenine, thymine, guanine, or cytosine.
Term
Replication fork
Definition
A Y-shaped region where the parent-DNA double helix is split into two single strands, which are then copied.
Term
single-stranded DNA-binding proteins (SSBPs)
Definition
attach to the separated strands of DNA and prevent them from snapping back into a double helix, during replication.
Term
topoisomerase
Definition
an enzyme that cuts DNA, allows it to unwind, and rejoins it ahead of the advancing replication fork.
Term
Primase
Definition

an enzyme that synthesizes a short stretch of ribonucleic acid (RNA) that acts as a primer for DNA polymerase. 

 

Primase is a type of RNA polymerase - an enzyme that catalyzes the polymerization of ribonucleotides into RNA. Unlike DNA polymerase, primase does not require a primer.

Term
DNA ligase
Definition
an enzyme that catalyzes the formation of a phosphodiester bond between the adjacent fragments
Term
telomere
Definition
region at the end of a linear chromosome 
Term
telomerase
Definition
an enzyme that catalyzes the synthesis of DNA from an RNA template.
Term
messenger RNA (mRNA)
Definition
carry information from DNA to the site of protein synthesis
Term
Transcription
Definition

The process of copying hereditary information in DNA to RNA.

 

DNA is trasncribed to RNA, by RNA polymerase.

Term
Translation
Definition

The process of using the information in nucleic acids to synthesize proteins.

 

Messenger RNA is translated to proteins, in ribosomes.

Term
genetic code
Definition
the rules that specify the relationship between a sequence of nucleotides in DNA or RNA and the sequence of amino acids in a protein.
Term
codon
Definition
a group of three bases that specifies a particular amino acid 
Term
mutation
Definition
Any permanent change in an organism's DNA. It is a modification in a cell's information archive - a change in its genotype. Mutations create new alleles.
Term
point mutation
Definition
single base change
Term
silent mutation
Definition
a point mutation that does not change the amino acid sequence of the gene
Term
DNA template strand
Definition
the strand that is read by the enzyme
Term
DNA coding strand
Definition
DNA strand that is not read by the enzyme; sequence of nucleotides matches the RNA sequence that is transcribed from the template strand and codes for a polypeptide
Term
holoenzyme
Definition
sigma + RNA polymerase; "whole enzyme"
Term
downstream of transcription
Definition
DNA that is located in the direction RNA polymerase moves during transcription is said to be 'downstream' from the point of reference
Term
upstream of transcription
Definition
DNA located in the opposite direction RNA polymerase moves during transcription.
Term
TATA Box
Definition
A unique sequence centered about 30 base pairs upstream of the transcription start site.
Term
basal transcription factors
Definition

Proteins that initiate eukaryotic transcription by binding to the appropriate promoter region in DNA.

 

Function is analogous to the function of the sigma proteins in bacteria, exept that many proteins are invoved, instead of one, and basal transcription factors are not part of a holoenzyme.

Term
elongation phase of transcription 
Definition

1. The process by which messenger RNA lengthens during transcription

 

2. The process by which a polypeptide chain lengthens during transcription

Term
Termination phase of transcription
Definition
In most cases, transcription stops when RNA polymerase reaches a DNA sequence that functions as a transcription-termination signal
Term
primary transcription (pre-mRNA)
Definition

When a eukaryotic gene is transcribed, the product is an immature primary transcript, or pre-mRNA.

 


Term
exons
Definition
The regions of eukaryotic genes that are part of the final mRNA
Term
intron
Definition
A region of a eukaryotic gene that is transcribed into RNA but is later removed, so it is not translated into a peptide or protein
Term
Splicing
Definition
As transcription proceeds, this proccess removes the introns from the growing RNA strand.
Term
small nuclear ribonucleoproteins (snRNPs)
Definition
In most cases, splicing is catalyzed by complexes of proteins and specialized RNAs, called small nuclear RNAs. These protein-plus-RNA complexes are known as snRNPs
Term
Spliceosome
Definition
In eukaryotes, a large, complex assembly of snRNPs that catalyzes removal of introns from primary RNA transcripts
Term
5' cap
Definition

As soon as the 5' end of a eukaryotic RNA emerges from RNA polyerase, enzymes add a structure called the 5' cap. 

 

The cap consists of the molecule 7-methyl-guanylate and three phosphate groups.

Term
poly(A) tail 
Definition
An enzyme cleaves the 3' end of most RNAs once transcription is complete, and another enzyme adds a long row of 100-250 adenine nucleotides, not encoded on the DNA template strand. 
Term
RNA processing
Definition
the general term for any of the modicfications, such as splicing or poly(A) tail addition, needed to convert a primary transcript into a mature RNA.
Term
ribosomes
Definition

A large complex structure that synthesizes proteins by using the genetic information encoded in messenger RNA strands. 

 

Consists of two subunits, each compsed of ribosomal RNA and proteins.

Term
polyribosome
Definition
A structure consisting of one messenger RNA molecule along with many attached ribosomes and their growing peptide strands.
Term
Transcripton/Translation in Bacteria
Definition

Transcription and translation can occur concurrently in bacteria because there is no nuclear envelope to separate the two processes. 

 

Thus transcription and translation are physically connected.

Term
transfer RNA (tRNA)
Definition

One of a class of RNA molecules that have an anticodon at one end and an amino acid binding site at the other. 

 

Each tRNA picks up a specific amino acid and binds to the corresponding codon in messenger RNA during transcription.

Term
aminoacyl tRNA synthetase
Definition
an enzyme that catalyzes the addition of a particular amino acid to its corresponding tRNA molecule
Term
aminoacyle tRNA
Definition
A transfer RNA molecule that is covalently bound to an amino acid
Term
anticodon
Definition
a set of three ribonucleotides that forms base paris with the mRNA codon
Term
ribosomal RNA (rRNA)
Definition
A RNA molecule that forms part of the structure of a ribosome
Term
ribosome binding site 
Definition
in a bacterial mRNA molecule, the sequence just upstream of the start codon to which a ribosome binds to initiate translation.
Term
initiation factors 
Definition
Proteins that mediate the initial interaction between the small subunit and the message
Term
Translocation
Definition
The process by which a ribosome moves down a messenger RNA molecule during translation
Term
elongation factors
Definition
Proteins involved in the elongation phase of tranlation, assisting ribosomes in the synthesis of the growing peptide chain.
Term
release factors 
Definition
Proteins that can trigger termination of RNA translation when a ribosome reaches a stop codon.
Term
release factors 
Definition

Protein that can trigger termination of RNA translation when a ribosome reaches a stop codon.

Term
transcriptional control
Definition

Occurs when regulatory proteins affect RNA polymerase's ability to bind to a promoter and initiate transcription

[image]

Term
Translation control
Definition
occurs when regulatory molecules alter the length of time an mRNA survives before it is degraded by ribonucleases, or affect translation intitiation, or affect elongation factors and other proteins during the translation process.
Term
post-translational control
Definition
Some proteins are manufactured in an inactive fomr and have to be activaated by chemical modification, such as the addition of a phosphate group.
Term
constitutively
Definition
genes that are transcribed all the time
Term
inducer
Definition
a substrate in a reaction and it stimulates the expression of a specific gene or genes.
Term
mutagens
Definition
chemicals that damage DNA and increase mutation rates
Term
replica plating
Definition
a method of identifying bacterial colonies that have certain mutations by tranferring cells from each colony on a master plate to a second (replica) plate and observing their growth when exposed to different conditions
Supporting users have an ad free experience!