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Pure Culture
Micro lab exam
9
Biology
Undergraduate 4
10/11/2011

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Term
Pure culture
Definition
Culture of bacteria that has the same DNA sequence
Term
Mixed culture
Definition
Different species of bacteria and/ or different DNA.
Term
What is the main purpose of pure culture?
Definition
To isolate a colony
Term
What are the three methods of isolating a colony?
Definition
Streak plate, pour plate and spread plate
Term
Which method is the best one to use to isolate a colony?
Definition
Streaking because the burning decreases the [conc.]
Term
Stock culture
Definition

Culture is innoculated and grown and stored at a low temperature that prevents growth.

Ex. plate, tubes and slants

 

Term
How long does a stock culture last for before it should be restreaked?
Definition
3 weeks
Term
working culture
Definition

is a slant that is maintained to innoculate media and to make smears for stains. 

A new working culture should be created every time it is used. 

[ a fresh culture]

Term
CFU 
Definition
  • Colonies forming unit
  • that represent the progeny of a single bacterium 
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