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Prokaryotic & Eukaryotic Cell
prokaryotes, eukaryotes, plasma membrane
48
Microbiology
Undergraduate 4
04/04/2010

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Term
prokaryotic cell structure
Definition
glycocalyx
- capsule (slime layer): layer of gelatinous material covering the cell.

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- composition varies with species: polypeptides, polysaccharides, glycoprotein
Term
function of glycocalyx (capsule or slime layer)
Definition
- ahesion to surfaces
- osmotic barriers (protection from pressure)
- protect bacteria from phagocytosis
- storage of nutrient
Term
prokaryotic cells
Definition
- pro = before karyon = kernel
- kingdom monera
- no true nucleus
- genetic material is in "nucleiod" region
- no membrane bound organelles
Term
flagella
Definition
fx. locomotion/motility
- peritrichous (all around)
- amphitrichous (both ends)
- monotrichous (one end)
- lophotrichous (a group, but going at one end)
Term
fimbriae and pili
Definition
- small hair-like projection
Term
function of fimbrae and pili
Definition
-sexual conjugation - pili form tubes for transfer of genetic material
- surface adhesion
-bacteriophage receptors - some viruses attach to pili
- NOT involved in motility
Term
function of cell wall
Definition
- maintains shape of the cell
- physical protection
- enables the cell to escape rupture (lysis) in a hypotonic environment
- lattice/net-like structure
- consists of peptidoglycan units that repeat over and over
Term
gram positive structure
Definition
- many layers of peptidoglycan
- forms a thick rigid structure
- contains techoic acid
Term
gram negative structure
Definition
-thin layer of peptidoglycan
- looser lattice (less rigid cell wall)
- high lipid content
- no teichoic acid (largely lipid)
Term
plasma membrane
Definition
- regulates diffusion in and out of cell
- contains active transport system
- site of enzymes involved in ATP production
Term
fluid mosaic model
Definition
- phospholipid bilayer
- a "dynamic" arrangement of phospholipids and proteins
Term
chromatophores
Definition
- contains chlorophyll
- only found in photosynthetic bacteria
Term
nuclear material
Definition
- 1 chromosome (DNA) loose in the cytoplasm in the nucleoid region
- bacteria often contain small circular, double-stranded DNA molecules called plasmids.
Term
ribosomes
Definition
- granules scattered throughout the cytoplasm
- protein synthesis, not membrane bound, organelle in both pro. and euk.
- protein synthesis = assemble a.a. into polypeptides
Term
cytoplasmic inclusions
Definition
-accumulated stored materials, valuable stored materials
-metachromatic granules (volutin) stored form of phosphate. primary in gram negative. take up stain more readily (suck up stain fast)
- polysaccharide granules
- lipid inclusions
Term
magnetosomes
Definition
-inclusions of iron oxide
- act like magnets
- may protect cells from accumulation of hydrogen peroxide
Term
endospores
Definition
-highly resistant stucture
- produced by certain bacteria
- all members of the family Bacillaceae produce endospores
- e.g. Genus Bacillus and Clostridium
- Resistance : the most resistant of all living cells
- highly resistant to: dessication (drying out), heat and toxic chemicals
- low metabolic activity (dormant)
- low water content
- thick spore coat
- heat resistant chemicals (calcium and dipicolinic acid)
- formation of endospores: not a reproductive process
- vegetative cell forms a single spore
- germinates to form one vegetative cell
Term
eukaryotic cells
Definition
- eu - true or good, karyon = kernel
- kingdoms: protista, plantae, fungi, animalia
- true nucleus containing genetic material
- contain membrane bound organelles
Term
cytoskeleton
Definition
-network of fibers that form a dynamic framework for support and movement
Term
microfilaments, actin filaments, intermediate filaments
Definition
Term
microtubules
Definition
- form: cilia, flagella, and centrioles
Term
organelles
Definition
- specialized structures that perform specific functions
- sequester reactions
- many contain folded membranes (increased surface area)
Term
nucleus
Definition
spherical or oval, contains DNA
Term
nuclear envelope/membrane
Definition
- double membrane perforated by pores
- maintains nuclear shape and regulates transport in and out of the nucleus
Term
nucleolus
Definition
- one or more spherical bodies within the nuclear envelope
- functions in the synthesis of ribosomal RNA
Term
rough ER
Definition
- studded with ribosomes
- site for synthesis of secretory proteins and membrane molecules
Term
smooth ER
Definition
- lack ribosomes
- synthesizes phospholipids, fats, and steroids
Term
ribosomes (euk.)
Definition
- formed of 2 subunits: 60S and 40S
- free ribosomes:suspended in the cytoplasm
- bound ribosomes: attached to ER
- sites of protein synthesis
Term
golgi apparatus
Definition
- flattened sacs, stacked on one another (pancakes)
- receive and modify products from the ER
- packages products in vesicles that transport, secretory, and storage
- lysosomes: membrane-enclosed sac of hydrolytic enzymes
- functions in phagocytosis, recycling cells own organic material
- programmed cell destruction
Term
lysosomal storage disease
Definition
- lysosomes lack one of the hydrolytic enzynmes
- i.e. Tay-Sachs diseas: lack enzyme to digest lipid. excess lipid accumulates and damages brain cells
Term
mitochondria
Definition
"power house of the cell"
- synthesis of ATP, sites of cellular respiration
- inner membranes folds into cristae
Term
plasma membrane
Definition
-semipermeable/selectively permeable
- controls what enters and leaves the cell
- maintains the cells "homeostasis" (homo = same; stasis = standing)
- keeps the internal chemical composition within narrow limits
Term
transport
Definition
the movement of materials across the plasma membrane
Term
passive transport
Definition
does not require energy from cell
Term
simple diffusion
Definition
molecules move from an area of greater concentraiton ot an area of lesser concentraion
Term
facilitated diffusion
Definition
a carrier protein combines with a specific substance and moves it across the membrane
Term
osmosis
Definition
the diffusion of water through a semipermeable membrane (water moves from a higher to lower concentration)
- water moves toward a region of higher solute (dissolved substance)concentration.
Term
osmoregulation
Definition
the control of water balance
Term
isotonic
Definition
-(iso = same; tonus = tension)
- concentration of dissolved substances are the same inside and outside the cell; no net flow of water
Term
hypertonic
Definition
-(hyper = above/exceeding)
- high solute concentration
Term
hypotonic
Definition
hypo = lower/below
- low solute concentration
Term
plasmoptysis
Definition
- (osmotic lysis) bursting of a cell in a hypotonic solution n(taking up the water)
Term
plasmolysis
Definition
- shrinkage of the cytoplasm due to loss of water in a hypertonic solution
Term
channel proteins
Definition
proteins which form channels through the membrane allowing passage of certain molecules.
- gated channels: some can act as "gates" that open and close
Term
active transport
Definition
-can move substances against their concentration gradient
- requires that the cell extend energy (ATP) e.g. sodium-potassium pump
Term
transport of large particles
Definition
- endocytosis
- exocytosis
Term
endocytosis
Definition
- phagocytosis (cell eating)
- pinocytosis (cell drinking)
- receptor-mediated endocytosis
Term
exocytosis
Definition
-vacuole fuses with plasma membrane and expels contents from cell
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