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Plaque
Micro lab exam
4
Biology
Undergraduate 3
11/12/2011

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Term
Purpose of Plaque count 
Definition
to determine titer [ conc.] of phage particles to orginal sample
Term
Characteristics of Viruses
Definition
  1. non-cellular (not considered as living)
  2. metabollically inert
  3. obligate intracellular parasites- rely on the host for reproduction 
  4. genetic info: DNA or RNA (single/ db strand) 
  5. genetic info: encased within a protein " capsid" 
  • helical 
  • icosahedral
Term
[image]
Definition

A. Head with DNA or RNA inside 

B. Collar

C. Tail

D. End plate

E. Tail fiber

Term
The five basic steps of phage replication
Definition
  1. Attachment: phage comes in contact with host cell
  2. penetration: Injects genetic material in teh cell
  3. Synthesis: phages are intracellular and hijack host machinery to synthesize
  4. assembly: packaging new phage particles (package in capsid) 
  5. lysis: release how cell (E.coli) lysis and releases new phage particles 
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