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plant science exam 1
plant science exam 1
65
Science
Undergraduate 3
02/19/2015

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Term
Isotopes
Definition
atoms of an element that have different numbers of neutrons. Some isotopes with extra neutrons are radioactive because the extra neutrons make the nucleus unstable. Isotopes are useful in tracing chemical reactions in plants and animals.
Term
Molecules
Definition
of two or more atoms joined by chemical bonds
Term
Compounds
Definition
contain two or more elements
Term
Covalent bonds
Definition
Form between atoms that share electrons; stronger than ionic bonds.
Term
Carbon why is it special?
Definition
has 4 electrons to share; sometimes it shares two electrons with an oxygen atom or another carbon atom; other times each of its electrons is shared with a different atom. Carbon can form many different compounds.
Term
Hydrogen bonds
Definition
The partial negative charge on one molecule is attracted to the partial positive charge on another molecule. DIFFERENT CHARGES DUE TO SIZES OF ATOMS
Term
Consequences of polarity of water molecules and hydrogen bonding
Definition
Highly effective solvent; Cohesion going up the trunk of trees; high specific heat, evaporates leaves & cools down; Highest density at 4ºC, ice expands and allows water to stay fairly constant underneath
Term
Primary Compounds
Definition
Carbohydrates, Lipids, Protiens, Nucelic acids
Term
Carbohydrates
Definition
cellulose, starch. 1C:2H:1O, Starch-energy, bonds are broken easily so energy can be obtained. cellulose are resistant to breakdown because alternate glucose subunits are flipped over, so cellulose is used for structural support. both made by glucose.
Term
Lipids
Definition
fats, oils, waxes, phospholipds. Carbon, Hydrogen, Oxygen, phorphorus in phospholipds. Fats and oils= made from glycerol and fatty acids that are long chains of CH2 groups. Energy storage in seeds and animals. fats=solid at room temperture, saturated, oils=unsaturated (double bonds btw c atoms) Phospholipids= similar structure to fats but one fatty acid is replaced by a phosphate group. fatty acids are nonpolar and hydrophobic; phosphate groups are polar and hydrophilic= lipid bilayer. Waxes – contain saturated fatty acids and another long chain molecule with OH groups; provide waterproof coating on stems and leaves.
Term
Proteins
Definition
large molecules composed of 20 different amino acids; carbon, hydrogen, oxygen, nitrogen and sulfur with a central carbon group bonded to a carboxylic acid group (COOH), an amino group (NH2), and a side chain (R); in water, proteins tend to fold so as to expose hydrophilic groups and enclose hydrophobic groups in the interior. joined by peptide bonds.
Term
4 levels of protiens.
Definition
Primary – sequence of amino acids; Secondary – helical or pleated sheet structures maintained by hydrogen bonds; Tertiary – folding of protein with shape maintained by covalent bonds between sulfur atoms; Quaternary – two or more polypeptide chains held together by hydrogen bonds.
Term
function of proiens
Definition
enzyme catalysts, Storage – protein stored in seeds helps new plants to start growth; animals consume these proteins and specifically require certain essential amino acids that they cannot make themselves;
Term
Nucleic Acids
Definition
large molecules composed of many nucleotide subunits.
Term
DNA composed
Definition
phosphate group; ribose (RNA) or deoxyribose (DNA); and bases – uracil (only RNA), thymine (only DNA), adenine, guanine, cytosine (both RNA and DNA).
Term
how are nucleotides bonded?
Definition
nucleotides in nucleic acids are attached to each other by covalent bonds through their phosphate groups.
Term
G+C=how many bonds? a+t=how many bonds? what type of bonds?
Definition
hydrogen bonds between bases: three bonds between guanine and cytosine and two bonds between adenine and thymine (uracil).
Term
nucleic acids functions
Definition
DNA forms the genetic material in cells and codes for certain amino acids to be put into proteins; a change in DNA (mutation) may cause different amino acids to be put into a protein chain and thereby affect the way the protein folds and how it functions.
b. RNA copies the information in DNA and does the work of protein synthesis.
c. ATP (adenosine triphosphate) is an important nucleotide that functions as an energy carrier; when its phosphate groups are broken, energy is released for cellular activities.
Term
Cell theory
Definition
all cells come from pre-existing cells Robert hooke=cork
Term
Compound light microscopes
Definition
Light passes through thin specimens that may be alive or stained with dyes to highlight certain features.
Term
Stereo dissecting microscopes
Definition
large thick specimens that light cannot pass through.
Term
Fluorescence microscopes
Definition
plant cells because chlorophyll and other plant structures are naturally fluorescent.
Term
Electron microscopes
Definition
Scanning – A beam of electrons passes over the surface of a specimen that has been coated with a thin layer of gold, platinum, or palladium; electrons bounce off the specimen and form an image of surface features.

and

Transmission – Specimens are preserved in plastic and extremely thin slices are cut and stained with lead (for lipids and proteins) and/or uranium (for DNA and RNA); electrons pass through the slice but are deflected by the heavy metals to form images of the cell’s components.
Term
Common features of all cells
Definition
cytoplasm & ribosomes
Term
Nucleus
Definition
Two membranes (nuclear envelope) enclose the nucleus; pores in the membrane allow communication between the nucleus and the rest of the cell.
Term
Nucleolus
Definition
makes ribosomes
Term
rough ER
Definition
has ribosomes on surface and synthesizes protiens
Term
smooth ER
Definition
fatty acids and phospholipds that breaks down toxins in the cell
Term
Golgi apparatus
Definition
CISTERNA; Golgi membrane pinches off to form a vesicle carrying the molecules.
Term
Cytoskeleton
Definition
Microtublues= tubule membrane
Microfilaments=actin
intermediate filaments
Term
Motor proteins
Definition
chemical energy into movement
Kinase= slow and steady (turtle-->duck-->K!)
Dynein= sliding movement
associated with microtubulin
Term
Mitochondria (DOZENS)
Definition
Cristae=contain enzymes and other molecules important for energy metabolism
Reproduce by binary fission like bacteria. Believed to be descended from ancient bacteria engulfed by another cell.
Term
peroxisomes
Definition
surrounded by single membrane; contain enzymes, one of which is catalase which breaks down hydrogen peroxide, a harmful product of metabolism.
Term
Cell walls
Definition
protect cell; contain cellulose (major structural component of cell walls composed of long cables) and non-cellulose (hemicellulose (binds cellulose fibrils together); pectin (combines with calcium and water to make jelly-like matrix between cells; used to make jam and jelly candy); proteins.)
Term
cell wall layers:
Definition
Primary and secondary
1. single layer cell wall of growing cells that maintains ability to stretch.
2. laid down inside the primary wall as cells mature; lignin may be added to the walls of water-conducting cells to make them impermeable to water
Term
Plasmodesmata
Definition
channels running through cell walls connecting the cytoplasm of adjacent cells; channels are lined with cell membrane and contain a strand of ER; can be permanently sealed if cells are damaged.
Term
Chloroplasts (40-50 each cell)
Definition
Internal membranes (thylakoids) contain chlorophyll and other molecules important for photosynthesis; watery stroma contiain other enzymes. Ribosomes and small amounts of DNA similar to those in prokaryotes.Reproduce by binary fission like bacteria.
Believed to be descended from ancient cyanobacteria.
Term
Other plastids
Definition
Amyloplasts – non-green plastids that store starch.
Chromoplasts – plastids containing carotenoid pigments that color leaves, flowers, and fruits red, orange or yellow.
Term
Central vacuole
Definition
large vacuoles characteristic of mature plant cells; important in maintaining turgor pressure; storage area for pigments, acids, sugars, heavy metals, tannins
Term
Membranes
Definition
enclose cell contents; composed primarily of phospholipids and proteins; important functions

involved in:
1. Communication – receptor proteins in membranes bind to chemical messengers, such as plant hormones, and transmit signals to cytoplasm
2. Transport of dissolved substances – gases and some small molecules pass easily through membranes; larger, charged molecules do not, unless they are transported by proteins.
3. Transport of larger or charged molecules – transport proteins in membranes are specific for certain kinds of molecules and bind with these molecules to move them through the membrane, e.g., membranes of plant root cells transport phosphate and nitrate from the soil into the cells .
Term
Transport of particles
Definition
endocytosis & exocytosis

water-osmosis
Term
chromosomes
Definition
histone protiens and dna

each chromosome occurs as a member of a homologous pair of chromosomes.

Each homologous chromosome contains genes for the same traits as the other homologous chromosome; since there are two chromosomes, these cells are diploid.

Some cultivated and wild plants contain four or six chromosomes with similar genes and are called tetraploid or hexaploid.
Term
Interphase
Definition
G1, S, G2
Term
G1
Definition
increases in size and synthesizes enzymes, ribosomes, membranes, microtubules and microfilaments.

Nucleus migrates to center of cell and is held in place by a sheet of cytoplasm (phragmosome) containing microtubules and filaments.

Checkpoint at the end of this phase, which may stop the cycle if conditions are not favorable, or may signal beginning of next phase.
Term
s PHASE
Definition
DNA and Histone replication.
Term
G2 Phase
Definition
final phase of rapid protein synthesis, especially of microtubules and filaments; at the end of this phase, DNA starts to condense to form chromosomes.
b. Checkpoint at the end of this phase, which may stop the cycle if conditions are not favorable or if there were mistakes in DNA replication.
Term
Mitoses
Definition
prophase, metaphase, anaphase, telephase
Term
Prophase
Definition
1. DNA first appears as threadlike strands and then condenses into chromosomes.
2. Each chromosome has been duplicated and the two identical “daughter” chromosomes (called chromatids) are seen joined together at the centromere.
3. Microtubules appear first in a random arrangement, then they line up along the spindle axis with tubules converging at the poles away from the equator of the spindle (which lies in the same plane as the phragmosome).
4. nuclear membrane begins to break down.


cancer drugs interfere with microtubules!
Term
Metaphase
Definition
Each chromatid develops a protein complex called a kinetochore that attaches to microtubules extending toward one pole; kinetochore of the sister chromatid attaches to microtubules extending to the opposite pole.
Microtubules tug chromatids in opposite directions so they tend to line up along the equator of the cell.
Cohesin proteins hold chromatids together.

taxol
Term
Anaphase
Definition
Sister chromatids separate at the centromere and move apart towards opposite poles; after separation, chromatids are called daughter chromosomes.
Term
Telophase
Definition
New nuclear envelop forms around the two new sets of chromosomes which uncoil and become less distinct.

Spindle disappears.
Term
Cytokenisis
Definition
phragmoplast (structure made up of microtubules and microfilaments) forms between 2 daughter nuclei.

secretory vesicles from the Golgi form a cell plate at the center of the cell; it then grows out toward the edges.
cell membrane and new cell wall form.
Term
meisos
Definition
remember: crossing over, in meristematic tissue, gametes, Haploid, nonjisdunction
Term
pyramidines vs purines
Definition
pyrimidines= C and T

Purines= G and A
Term
findings of DNA
Definition
CHargaff= A=T

watson, crick, franklin= helical
Term
DNA stuff
Definition
complementary, not identical

Expressed as kilobases (kb, thousands of base pairs); 1 kilobase contains about enough information to code for one average protein.
Term
Damage repair
Definition
DNA polymerases can reverse themselves and go back to repair damage during replication; DNA repair nucleases can cut out damaged pieces of DNA and then put in the correct bases; DNA ligase connects the repaired section to the main strand.
Term
RNA
Definition
MRNA; TRNA; rRNA

base + ribose sugar + phosphate

RNA molecules are usually single stranded but some parts of RNA molecules may be folded to have sections that are double stranded
Term
mRNA synthesis (transcription)
Definition
DNA--> RNA

RNA polymerases attach at a promoter region on DNA, open up the DNA double helix, and complementary nucleotides are added.

In eukaryotes, mRNA must be processed before leaving the nucleus.
a. Sequences that do not code for protein (introns) are cut out.
b. A cap is added at one end and a tail containing a series of adenine bases is added at the other end to prevent degradation by RNAses in the cytoplasm.
Term
rRNA
Definition
Ribosomes are made of protein and RNA (rRNA); there are two subunits: a smaller one with a binding site for mRNA and a larger one with 3 binding sites for tRNAs.
Term
tRNA
Definition
One of the free ends of the tRNA has an attachment site for an amino acid; a specific enzyme recognizes the shape of the tRNA and attaches the correct amino acid .

In the loop opposite the free ends, there is a group of 3 bases called the anticodon: these are complementary to the codon for the amino acid that is attached to the tRNA.
Term
Protein Synthesis (Translation)
Definition
Initiation, Elongation, Termination
Term
Initiation
Definition
An initiator tRNA containing methionine and a mRNA attach to the small ribosomal subunit with the anticodon of the tRNA attaching to the start codon on the mRNA.

The larger ribosomal subunit attaches to the smaller subunit.
Term
Elongation
Definition
tRNAs carrying their amino acids attach to the ribosome and to the mRNA codons complementary to the tRNA anticodons.

Peptide bonds are formed between the amino acids, and the ribosome moves down the mRNA.
Term
Termination
Definition
At a stop codon, a cytoplasmic protein binds to the mRNA and the ribosomal subunits separate and mRNA and the protein are released.
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