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3 Photosynthesis: Calvin Cycle
June 25
22
Biology
Undergraduate 2
06/25/2015

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Term
Describe Light harvesting
Definition
Light excites electrons
 Energy passed from one
chlorophyll to the next, into the
Reaction Center
 Only energy is transferred – no
electrons change hands
Term
Describe the Reaction-center complex
Definition
◦ Accumulating energy frees a
pair of electrons from
Reaction Center chlorophylls
◦ Transferred across complex
to primary electron acceptor
 Electrons replaced from H2O,
making O2
Term
What is linear electron flow?
Definition
A series of redox reaction pass these electrons to plastoquinone and down the electron transport chain to reaction center chlorophylls of Photosystem

or

A series of Redox reactions pass these electrons to
plastoquinone and down the electron transport chain to
reaction center chlorophylls of Photosystem I.
The process repeats itself in PSI, using accumulated light
energy to pass the electrons to Ferredoxin (the primary
electron acceptor for PSI), then to NADP+ to form NADPH
The ETC transfers protons from Stroma to Thylakoid Space
This establishes a proton gradient, then used by ATP
synthase to generate ATP
Term
Know the difference between the Electron Transport Chain in Mitochondria and Chloroplast
Definition
Term
Where does the Calvin cycle occur?
Definition
in the stroma of chloroplasts
Term
What kind of energy does the Calvin cycle use?
Definition
ATP and NADPH produced in light reactions
Term
What forms during the Calvin cycle?
Definition
Forms glyceraldehyde-3-phosphate (G3P)
 Building block for more complex sugars
Term
How many calvin cycles does it take to make 1 G3P?
Definition
3 cycles
Term
Describe the electron transport and ATP
Definition
◦ The ETC transfers protons from Stroma to Thylakoid Space
◦ This establishes a proton gradient, then used by ATP
synthase to generate ATP
Term
Describe the generation of ATP in the ETC
Definition
◦ Electron Transport Chain
establishes a proton gradient
◦ ATP synthase harvests the energy
to make ATP
Term
What are the 3 phases of the Calvin cycle?
Definition
◦ Carbon fixation
◦ Reduction of sugar
(output)
◦ Regeneration of
electron acceptor
Term
Describe carbon fixation in the calvin cycle
Definition
◦ Carbon fixation
 CO2
is attached to Ribulose bisphosphate (RuBP) (5-C sugar)
 Catalyzed by enzyme Rubisco – most abundant protein on the
planet
 Resulting 6-C sugar is inherently unstable
 Splits to 2 3-C sugars
Term
Describe reduction of the calvin cycle
Definition
◦ Reduction
 3-C sugars are phosphorylated from ATP
 3-C sugars are reduced by NADPH
 Produces 3-C sugar
 1/6 leaves pathway as G3P
 remaining 5/6 of G3P required to
regenerate RuBP
Term
Describe regeneration of the calvin cycle
Definition
◦ Regeneration
 5 3-C sugars rearranged to 3 5-C sugars
 1 phosphate from ATP added to each 5-C sugar,
forming RuBP are formed
Term
Describe what is made and what is used/needed in the calvin cycle
Definition
◦ 1 G3P produced for every 3 CO2
 This makes sense: 3 individual
carbons enter, 1 3-C (G3P) leaves
for each cycle
Figure 10.19
 3 RuBP (5-C) plus 3
CO2
(18 total C) are
needed to make 6
G3P (3-C) (18 C)
 5 G3P (3-C) are
needed to make 3
RuBP (5-C) to
restart
 Each cycle uses 6
NADPH and 9 ATP
Term
What is the fate of G3P in the Calvin Cycle?
Definition
◦ Transported to cytoplasm
◦ Used to make:
 Glucose
 Sucrose
 Etc.
Term
How are plants divided?
Definition
By how they enter the calvin cycle
Term
What are C3 plants?
Definition
– (rice, wheat, soybeans)
 CO2
is initially fixed by Rubisco, forming
a 3-C compound in Calvin Cycle
Term
What are C4 plants?
Definition
(sugarcane, corn, grass)
 CO2
, initially fixed by different enzyme,
forming a 4-C sugar, oxaloacetate. It is
later stripped off to enter Calvin Cycle
 These reactions take place in different
cell layers: carbon fixation in mesophyll
and Calvin Cycle in Bundle-sheath cells
 Pass between two via plasmosdesmata
Term
Describe photosynthesis in arid conditions
Definition
 Stomata kept closed to reduce H2O loss
 O2 builds up, CO2 depleted
Photorespiration
Rubisco can substitute O2
for CO2
Produces CO2
Uses ATP, no sugar production
Term
What can some C4 plants do?
Definition
separate steps temporally
Term
Describes the cyclic electron flow
Definition
◦ Ferredoxin requires NADP+ in order to make NADPH
◦ If cell is depleted of NADP+ then ferredoxin can pass electrons
back to Electron Transport Chain, making more ATP but no NADPH
 Energy but no photosynthesis
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