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Phase-Contrast Microscopy
Phase-Contrast Microscopy
12
Microbiology
Undergraduate 2
10/26/2011

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Cards

Term
If amplitude is reduced ...
Definition
... object appears dark.
Term
Phase shift:
Definition
light slowed down by 1/4 wavelength
Term
Direct rays...
Definition
... rays that pass straight through unaffected by the medium
Term
Direct rays are unaltered in amplitude and phase.
Definition
True!
Term
Diffracted rays ...
Definition
... Rays that are bent because they are retarded by the medium.
Term
Coincidence ...
Definition
direct and diffracted rays are brought into exact phase with each other.
Term
Interference ...
Definition
... 2 light waves of equal amplitude are in reverse phase => amplitudes will cancel each other to produce dark object.
Term
The Zernike microscope differs from Brightfield  in that:
Definition

1. different type of diphragm

2. phase plate

Term
What does an annular stop do?
Definition
Only allow a hollow cone of light to pass through the condenser to the specimen.
Term
What does the phase plate do?
Definition
It has a phase ring that advances or retards the direct light rays 1/4 wavelength.
Term
Amplitude summation ...
Definition
getting a brighter image resulting from the convergence of diffracted and direct rays on the image plane.
Term
The image in a phase-contrast microscope will be 4 times brighter than with a Brightfield microscope.
Definition
True!
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