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P401
Asbestos exam
226
Environmental Studies
Professional
10/30/2023

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Term
Five properties of asbestos that made it commercial in building trades
Definition
Flexibility, high tensile strength,
incombustibility, low thermal conductivity, and resistance to chemical attack
Term
CAR defines asbestos as any of
the following fibrous silicate minerals?
Definition
Chrysotile crocidolite, amosite, asbestos anthophyllite,
asbestos actinolite or asbestos tremolite
Term
Silicate minerals are classified by?
Definition
the number and arrangement of silicate tetrahedra in the
repeating units of the crystal lattice
Term
Chrysotile is classified as a sheet silicate and is a member
of which group?
Definition
serpentine
Term
Types of asbestos in the amphibole Group
Definition
crocidolite, amosite, anthophyllite,
actinolite or tremolite
Term
low percentages of asbestos can be
present in other mined products such as?
Definition
Talc or Iron ore
Term
Type of microscope used for the initial examination of the sample
Definition
low-power stereo-microscope
Term
Fibres observed in the course of stereo microscope examinations are categorised tentatively on...
Definition
the basis of morphology and
certain physical properties
Term
Fibres suspected of being Asbestos are..
Definition
mounted in a refractive index (RI) liquid chosen to match the most likely
asbestos type
Term
The second microscope used to identify the asbestos type?
Definition
polarised light microscopy (PLM)
Term
PLM microscope magnification
Definition
from about 80x upwards, as appropriate to the type of sample
Term
Finish the sentence...

If samples are
non-homogeneous and consist of layers of different material or are samples of debris
made up of several different materials:
Definition
Homogenous each layer or material will need to be examined
Term
If no fibers are present under the Stereo or PLM which test would be used next?
Definition
The pinch test
(, additional searches for small
asbestos fibres on random sub-samples of a few milligrams are undertaken using PLM )
Term
what is recommended for temporary containment of
sample waste before final disposal
Definition
Heavy-duty plastic bags in correctly labeled bags
Term
Where must stereo-microscope examinations and sample preparation be executed
Definition
a fume cupboard, or in a suitable cabinet
Term
What is the minimum face velocity of fume cabinets
Definition
0.5 m/s over the working area
Term
What class vacuum cleaner is also recommended for emergency clean-up and cleaning up spills
inside the cabinet
Definition
H
Term
Apparatus required for sample preparation?
Definition
probes, needlepoint tweezers and fine
tungsten needles
Term
Pre-treatment apparatus may include?
Definition
glass beakers, disposable or washable glass
Petri dishes or containers, an ultrasonic bath, boiling tubes, vacuum filtration flask, pump and filter
holder with appropriate filters,
Term
What may be needed to break samples
Definition
Pliers, a file, a hammer and a saw may be needed to break samples a pestle and mortar may be used to
grind samples and release fibres from matrices.
Term
What happens to wet samples in sample prep
Definition
Wet samples may be dried using a hot plate, oven,
infra-red lamp or by flushing through with acetone
Term
What equipment is needed for PLM Analysis
Definition
glass slides, coverslips, probes
and tweezers of appropriate quality are required including PLM Microscope and RI liquids
Term
What equipment is used to assess whether organic
fibres and spider’s webs are present?
Definition
A flame
Term
What is a homogeneous sample
Definition
Sample with one layer
Term
What is the process with non-homogeneous samples
Definition
Record sample as non-homogeneous then describe each individual layer by colour,
texture, and if possible, material type). Take sub-samples if necessary
Term
What is the magnification of a stereo microscope
Definition
8–40x magnification
Term
What are the two slides on the PLM microscope used for analysis that are removable
Definition
a removable analyser and a removable first-order red compensator
Term
What following accessories are required to aid the assessment of fibre RIs by producing
intense dispersion staining colours
Definition
a phase contrast objective (10x magnification or greater) and condenser with matching centrable phase annuli

or a dispersion staining objective (10x magnification) with a central stop in its back focal plane, used
in conjunction with the condenser iris
Term
What type of illumination is needed
for fibre identification:
Definition
Köhler-type illumination
Term
What products, may contain asbestos
fibres that are too fine to be detected in this
initial examination
Definition
products such as decorative plasters, vinyl floor
tiles, and settled dusts
Term
what determines the sample treatment that is required to help isolate the fibers from the other components
Definition
The product type determined by The appearance, colour and texture of the sample, and any fibre types observed
Term
Do you have to do sample preparation in a sample where no fibres are present but is a material/product type that is known to have the potential to contain asbestos
Definition
One or more sample prep techniques should be carried out
Term
What type of sample prep is recommended for loose fibre, sprayed coatings and textiles
Definition
Usually sample prep isn't required
Term
What type of sample prep is recommended for lagging?
Definition
Breaking and treat with dilute HCl
Term
What type of sample prep is recommended for Asbestos insulating board (AIB), asbestos
cement (AC) and millboard
Definition
Usually not required
Term
What type of sample prep is recommended for Decorative plaster, textured decorative coatings
Definition
Breaking and acid treat with dilute HCl
Term
What type of sample prep is recommended for Mastics and adhesives
Definition
Breaking, solvent treatment (acetone) and/or combustion
Term
What type of sample prep is recommended for Floor tile
Definition
Breaking and solvent treatment or combustion
Term
What type of sample prep is recommended for Vermiculite
Definition
Combustion/heating and/or acid/alkali digestion/sedimentation
Term
What type of sample prep is recommended for wet samples
Definition
May be searched while damp but dry fibres are required for PLM analysis (heat lamp)
Term
Which bit of the sample may be inspected under the stereo microscope after breaking
Definition
The newly fractured edges should be inspected
under the stereo-microscope for the presence of protruding fibres, which are carefully removed and
mounted in an appropriate RI liquid for PLM analysis
Term
What types of acid are used in sample prep
Definition
Dilute acetic acid (eg 50%) or cold dilute hydrochloric acid (eg 10%)
Term
What do you wash the sample with after acid treatment to reduce drying time
Definition
Acetone
Term
The acid is used to remove:
Definition
e calcium carbonate, calcium sulphate and calcium silicate, which are
common binders in insulation and asbestos boards, and which are used as fillers in floor tiles
Term
Fibre release when treated with acid may be aided by
Definition
stirring, crushing with a suitable tool or by ultrasonic treatment
Term
Solvent treatment works on...
Definition
Organic binders (eg in plastics, bitumen, resin or rubber products)
may require prolonged treatment in solvents
Term
Ashing/combustion treatment is used to remove...?
Definition
organic binders
Term
What can be used for ashing/combustion treatment
Definition
400°C in a furnace/oven or using a spirit lamp or a lighter
Term
How can you do Disaggregation treatment
Definition
These can be removed by various
treatments eg. placing in denatured alcohol,
or using pressure on the coverslip to separate fine attached particles in the RI liquid
Term
Sedimentation treatment
Definition
allows particle size separation to take place and various
fractions to be selected for more detailed analysis. Some materials and particles can also be separated by flotation
Term
Commonly known as White asbestos
Definition
Chrysotile
Term
Commonly known as Brown Asbestos
Definition
Amosite
Term
Commonly known as Blue asbestos
Definition
Crocidolite
Term
Soft with bundles
of sinuous fibres
Definition
Chrysotile
Term
Soft or harsh; may appear as easily visible
parallel fibre bundles
Definition
Amosite, Anthophyllite, and Tremolit
Term
Asbestos types with Soft or harsh with parallel fibre bundles
Definition
Actinolite and Crocidolite
Term
Silky Lustre
Definition
Chrysotile
Term
Vitreous lustre
Definition
Amosite and Anthophyllite
Term
Vitreous/silky Lustre
Definition
Tremolite
Term
Vitreous/green/grey Lustre
Definition
Actinolite
Term
Metallic (dark and highly reflective) Lustre
Definition
Crocidolite
Term
High Tensile Strength
Definition
Chrysotile, Amosite and Crocidolite
Term
Medium Tensile Strength
Definition
Anthophyllite
Term
Low Tensile strength
Definition
Tremolite and Actinolite
Term
RI Liquid for Chrysotile
Definition
1.550
Term
RI Liquid for Amosite
Definition
1.670
Term
RI Liquid for Anthophyllite
Definition
1.605
Term
RI Liquid for Temolite
Definition
1.605
Term
RI Liquid for Actinolite
Definition
1.640
Term
RI Liquid for Crocidolite
Definition
1.700
Term
inelastic type of asbestos
Definition
Chrysotile
Term
Elastic type of asbestos
Definition
Amosite, Anthophyllite, Tremolite, Actinolite, and Crocidolite
Term
Before mountain in RI liquid fibres should be
Definition
dry and relatively free from other particulate matter
Term
Trace asbestos term can be used when
Definition
during the search of the two ‘pinch’ samples by PLM only 1 or 2 fibres (or fibre bundles) are seen and identified as asbestos
Term
How long should you look under the Stereo Microscope?
Definition
10 minutes
Term
In the pinch test what is the minimum time you should look under the PLM Microscope
Definition
5 minutes per mounted microscope slide where there is at least two extracted preporations
Term
asbestos not detected’ should be reported when...
Definition
No asbestos fibres or fibre bundles
are identified after areful searching of the sample under the stereo-microscope for around 10 minutes; and subsequent searching a minimum of two extracted preparations mounted in suitable RI
liquid at high magnification by PLM/PCM for a further 5 minutes.
Term
Why must a number of optical properties be assessed to identify a particle
Definition
The optical properties viewed will depend on fibre type and particle orientation which may differ due to The exact arrangement of the silica tetrahedra and the type of cations in the structure
will change the interactions (optical properties) between the crystal structure and the light rays transmitted through them
Term
What settings are needed on the PLM to test pleochroism
Definition
plane-polarised light conditions - polariser only (no analyser or 1st order red compressor)
Term
what do you look for when the PLM is set up for Plane-polarised light (polariser only)
Definition
Colour and pleochroism (if present)
Term
what do you look for when the PLM is set up for Polarised light/crossed polars polariser and analyser)
Definition
Birefringence (anisotropic behaviour)
Extinction characteristics
Term
what do you look for when the PLM is set up for Crossed polars with first-order red compensator
Definition
Sign of elongation
Term
what do you look for when the PLM is set up for using a phase contrast, or dispersion staining objective with polariser only
Definition
Refractive index by dispersion staining colours
Term
Which asbestos type has blue/grey pleochroism?
Definition
Crocidolite
Term
Which asbestos type has Green/grey pleochroism?
Definition
Actinolite
Term
Which two asbestos types don't have moderate Birefringence and which type do they have?
Definition
Chrysotile - Low

Crocidolite - Low/anomalous
Term
Which Asbestos type has Complete, or undulose with curved fibres; parallel extinction
Definition
Chrysotile
Term
Which aspestos types have Complete parrallel or small angle extinction?
Definition
Tremolite and Actinolite
Term
Which type of asbestos has a negative fast sign of elongation
Definition
Crocidolite
Term
Why may sign of elongation change in asbestos types?
Definition
Heat damage
Term
Chrysotile dispersion staining objective colours
Definition
Purple/ Blue
Term
Amosite dispersion staining objective colours
Definition
Yellow/ Purple-red
Term
Anthophyllite dispersion staining objective colours
Definition
Yellow–orange/ Blue Red
Term
Tremolite dispersion staining objective colours
Definition
Yellow/ Blue
Term
Actinolite dispersion staining objective colours
Definition
Red- Orange - yellow/ Blue - Purple
Term
Crocidolite dispersion staining objective colours
Definition
Blue/ Blue
Term
What is the Birefringence
Definition
The numerical difference between the highest and lowest RIs of a mineral is known as the birefringence
Term
Why does Crocidolite have an anomalous interference colours from grey to pale blue or sometimes brown
Definition
Crocidolite has a low birefringence and strong pleochroism (blue colour) which results in
anomalous interference colours from grey to pale blue or sometimes brown.
Term
How is extinction caused
Definition
Extinction is caused
when a vibration direction within the fibre/particle is in alignment with the vibration direction of the
illuminating rays. All light is transmitted without alteration as a single refractive index and hence,
when it meets the analyser, it is stopped
Term
where will crossed polars 'extinguish'
Definition
at four positions each 90° apart, while at
45° between each extinction position, interference colours should be visible
Term
When is a fibre described as positive or length slow
Definition
If the high RI vibration plane (slow ray) is parallel to the long axis, then the fibre is described as positive
Term
When is a fibre described as negative or length fast
Definition
if the low RI vibration plane (fast ray) is parallel to the long axis, the fibre is described as negative (or length fast)
Term
What may change the sign of elongation
Definition
exposure to heat of about 300°C or higher may change the sign
of elongation of crocidolite to positive (length slow)
Term
What is the refractive index (RI)
Definition
is the ratio of the speed of light in a vacuum to the speed of light in that substance and is
generally quoted for the sodium D line (nD) at 589 nm
Term
Why do crystalline materials have more than one refractive index
Definition
Most crystalline
materials have more than one refractive index because the atoms are packed in a different array along the three crystal axes
Term
One or more observations are conducted to determine whether the RI of the fibre is higher than, lower than or equal to that of the mounting liquid by...
Definition
Relief
Becke line
Dispersion Staining colours
Term
What is high Relief
Definition
high relief denotes high contrast
and, conversely, low relief denotes low contrast
Term
What colour appears when the particle and mount are a long way from RI match point
Definition
White (saturated colours on a black background)
Term
What colour appears when the RI of the mounting liquid is too high
Definition
a pale blue colour (saturated colours on a black background)
Term
What colour appears when a higher RI mount should be used to achieve a match in
the visible spectrum.
Definition
a pale yellow colour indicates (saturated colours on a black background)
Term
What was Chrysotile most used in
Definition
cement and textile products
Term
What was crocidolite most used in
Definition
insulation and as a spray coating due to its thermal properties
Term
What is Amosite most used in
Definition
board products and ceiling tiles
Term
How many deaths are caused by asbestos annually
Definition
4,500
Term
How many buildings are predicted to still contain asbestos?
Definition
between 500,000 and 2m commercial premises and around 2.5m domestic dwellings
Term
What risk is determined largely by asbestos exposure before age 30
Definition
Mesothelioma
Term
Why are carpenters at a particularly high risk?
Definition
exposure to amosite while cutting AIB
Term
Which asbestos type is hydrophilic
Definition
Chrysotile
Term
what % asbestos fibre does 'limpet' or sprayed asbestos-cement have in it
Definition
85%
Term
Why is limpet high risk
Definition
high content and high friability
Term
when was the statutory ban on brown and blue asbestos
Definition
1985
Term
when was the statutory ban on white asbestos
Definition
1999
Term
What risk is thermal insulation regarded as
Definition
high/medium risk
Term
What content did thermal insulation have of asbestos
Definition
15 to 80%
Term
What type of asbestos did thermal insulation contain
Definition
blue, brown or white asbestos
Term
what risk is AIB
Definition
medium risk
Term
What percentage content of asbestos is in AIB
Definition
15 to 25%
Term
What risk is asbestos cement
Definition
Low risk
Term
What abestos content is usually n asbestos cement
Definition
10 to 15%
Term
What makes asbestos cement low risk
Definition
lower content, its low friability as very dense and fibres firmly bound in cement matrix and it was usually only white asbestos used.
It is very resistant to weathering and With the material predominantly being used externally,
any fibre release is immediately diluted in atmosphere.
Term
What are the three main diseases caused by asbestos
Definition
Asbestosis. Scarring or fibrosis of the lung.
Mesothelioma. Cancer of the pleura or peritoneum.
Asbestos related lung cancer
Term
What are three benign conditions
Definition
Pleural plaques
Pleural thickening and effusions
Asbestos warts and corns
Term
What are the three main determinants of risk
Definition
a) Ease of fibre release (loosely or firmly bound)
b) % content of fibre in the product itself
c) Type of fibre – blue brown and white.
Term
What does this list display
1. Sprayed coatings
2. Insulation
3. Asbestos insulating board (AIB)
4. Asbestos cement (AC)
5. Textiles
6. Composites
Definition
asbestos products in descending order of risk
Term
What is the control limit of asbestos
Definition
0.1 f/cm3 (same as f/ml) averaged over a continuous 4 hour period
Term
What is the exposure Limit of Sporadic and low intensity exposure
Definition
0.6 f/cm3 over 10 minutes
Term
What are some controls if the work area is over the control limit
Definition
The work must be notified to HSE / Local Authority

All employees undertaking this work must be under
medical surveillance and their exposure should be monitored

eating, drinking
and smoking in this area is prohibited

decontamination unit (DCU) and the employer must minimise the
number of people entering the area
Term
What is the Clearance indicator
Definition
“Lowest level reliably detectable above
background”. This level has no relation to “safety”
Term
Typical compositions of spray coating
Definition
85% crocidolite or amosite or amosite + chrysotile mixture
Term
Typical Composition Pipe insulation - hard set
Definition
up to 70% of all or any mixture of asbestos types other non-asbestos fibres (hair, straw, wood, cotton)
Term
Typical Composition Pipe insulation – sectional
Definition
15 to 60% amosite mainly, but also mixtures
Term
Typical composition Asbestos cement
Definition
10 - 15% chrysotile may rarely find Amo and Croc
Term
Typical composition Asbestos insulating board (AIB)
Definition
15 - 25% amosite (earlier up to 40%)
• may contain some chrysotile in combination with amosite
• can be crocidolite alone
Term
Roofing felt composition
Definition
5% chrysotile
Term
Floor tiles composition
Definition
5 - 10% chrysotile
Term
Gaskets composotion
Definition
95% chrysotile
Term
Rope or textiles composition
Definition
100% chrysotile or blended with synthetic
organic or cellulose fibres
Term
Eternit window boards composition
Definition
~ 10% chrysotile
Term
Toilet cisterns compsition
Definition
10% amosite (chrysotile and/or crocidolite)
Term
Textured coatings composition
Definition
0.5 - 3% chrysotile
Term
slow speed =
Definition
High RI
Term
Birefringence
Definition
the numerical difference between the highest and lowest RI along three axes
Term
RIalong > RIacross
Definition
length slow or positive sign
of elongatio
Term
RIalong < RIacross
Definition
length fast or negative sign of elongation
Term
Interference colours
Definition
When a fibre is rotated at 45º it appears white to grey At vertical and horizontal (parallel to planes of polarisation) it is
‘extinguished’
Term
Crocidolite often shows ... under
crossed polars
Definition
‘anomalous interference colours’
Term
Crocidolite often shows ... under
crossed polars
Definition
‘anomalous interference colours’
Term
Angle of extinction
Definition
Normally optical plane coincides with geometric plane
Fibre is ‘extinct’ parallel to planes of polarisation
For some crystal systems the optical plane is inclined to the crystal
plane
Term
angle of extinction Chrysotile, amosite and crocidolite
Definition
parallel
Term
angle of extinction Tremolite and actinolite
Definition
oblique extinction angles
• may be difficult to observe, HSG 248 says ‘parallel or small
(5º) angle of extinction’
Term
Angle of extinction Anthophyllite
Definition
orthorhombic and always parallel extinction
Term
How is the z´/ g direction always aligned and marked
Definition
NE/SW
Term
What colour would you expect to see Slow directions parallel
Definition
2nd order blue
Term
What colour would you expect to see Slow directions perpendicular
Definition
1st order yellow
Term
Pleochroism
Definition
Differential absorption of visible wavelengths with orientation in plane polarised light
Term
pleochroic types of asbestos
Definition
crocidolite and actinolite
Term
Becke Line
Definition
White fringe at particle interface with liquid of different RI
Term
McCrone Objective
Definition
Intersection points represent wavelengths (colours) at which particle and fibre match exactly - λ0
Term
When looking at the McCrone Objective if RI fibre > RI liquid what colou would you expext
Definition
yellow/orange colour
Term
When looking at the McCrone Objective if RI fibre < RI liquid whqt colour would you expect
Definition
blue colour
Term
Chrysotile dispersion colours can depend on
Definition
source and history (acid treatment or weathering)
Classically: blue to purple
Term
Organic fibres which may mimic chrysotile
Definition
Leather
• Polypropylene or polyethylene
• Spiders’ web
• Paper swarf
• Feathers?
Term
What would you do if you didn't know if a fibre was asbestos or another material
Definition
Check with flame
Term
How would you define AIB
Definition
any flat sheet, tile or building board consisting of a
mixture of asbestos and other material except asbestos
cement
Term
How would you define AC
Definition
“asbestos cement is a material which is predominantly a
mixture of cement and chrysotile which absorbs less than 30%
water by weight
Term
How to test if the sample is AIB or AC
Definition
Water Absorption Test
Term
What is the Water Absorption Test process
Definition
Take a 3 x 3cm sample
Dry it and weigh it
Immerse in water for 15 mins
Remove and re-weigh
If weight of water absorbed is <30% of original weight, report as AC
If >30% report as AIB
Term
How many samples can you have in a cabinet at any time
Definition
One sample only in cabinet at any one time
Term
What is the aim of sample handling
Definition
to prevent cross-contamination
Term
Where can sample bags be opened
Definition
only inside cabinet
Term
What should you check on the cabinet before use
Definition
Check airflow – should be 0.5 to 1.0 m/sec
Term
How often does air testing in lab need to be held
Definition
Monthly
Term
Where must chemicals be kept
Definition
should be kept in lockable metal cabinet and decanted
into smaller containers for use in cabinet
Term
When must cabinmates be cleaned
Definition
preferably between samples but especially at the end of the day using type-H vacuum
Term
How must waste be removed
Definition
via red bags and licensed contractor
Term
When does pleochroism develop within amosite
Definition
when it is heated
Term
What happens to Croc and Amo after being exposed to temps over 500°C
Definition
effectively indistinguishable by light microscopy as RIs are greater than 1.700
Term
What happens to chrysotile after being exposed to heat over 600°C
Definition
the birefringence decreases and the fibres become pale yellow-brown
changes the sign of elongation to negative (length fast) when the RI changes to above 1.640
Term
What happens to the RI of the asbestos when there is heat damage
Definition
it increases
Term
The purpose of a QA programme
Definition
sampling, analysis, recording and
reporting of the results all meet acceptable standards
Term
What percentage of samples should be routinely checked for QC
Definition
5% of re-analyses on new samples should be maintained (ie the samples analysed up to the maximum number of 20 and 40
Term
What is the maximum points or samples done within 24 hours
Definition
60 samples or 70 points
Term
maximum number of samples from category A (ACM) until additional QC applies
Definition
40
Term
maximum number of samples from category B (No asbestos detected and harder to detect materials) until additional QC applies
Definition
20
Term
[image]
Definition
hazard placard to be displayed on the sealed asbestos container
Term
Limited quantities exemptions or, in specific cases, the bonded materials Asbestos can be contained within:
Definition
samples should be double-bagged (with the bags individually sealed) and then placed in an allowed outer packaging which bears the asbestos warning label
Term
What should asbestos waste bear
Definition
A warning lable
Term
Where should asbestos be binned when it contains 0.1% w/w or more asbestos or
a piece of asbestos large enough to be visible to the naked eye
Definition
hazardous/special waste
Term
When is asbestos hazardous waste
Definition
After they have been analysed
Term
Achromat
Definition
A microscope objective in which chromatic aberration is
minimised for two wavelengths (one less than about 500 nm,
and the other greater than about 600 nm), and spherical
aberration and other aperture-dependent effects are
minimised for another wavelength (usually about 550 nm).
Term
Analyser
Definition
A polar used after the object (usually between the objective
and the primary image plane) to determine optical effects
produced by the object on the light, polarised or otherwise,
with which it is illuminated.
Term
Becke line
Definition
A bright line (due to refraction and/or diffraction) formed in
the image at the boundary between media of different
optical path lengths. It moves in the direction of the longer
optical path when the distance between the objective and
the object is increased. (Note: this phenomenon is used to
recognise relative differences in Rl of two adjacent media,
eg a particle and the surrounding medium; when the Rls are
matched the Becke line disappears.)
Term
Bertrand lens
Definition
An intermediate lens which transfers an image of the back
focal plane of the objective into the primary image plane;
used for conoscopic observation in polarised light microscopy
and for adjustment of the microscope illumination system,
especially with phase contrast microscopy.
Term
Birefringence
Definition
The qualitative expression of the maximum difference in Rl due to double refraction (symbol n)
Term
Compensator
Definition
A retardation plate (sometimes of variable optical path length difference) used to measure the optical path length differences within an object
Term
Condenser
Definition
A part of the illumination system of the microscope which
consists of one or more lenses (or mirrors) and their mounts,
usually containing a diaphragm, and is designed to collect,
control and concentrate radiation
Term
Crossed polars
Definition
The condition in which the vibration directions of polars
(polariser and analyser) are mutually perpendicular.
Term
Dispersion-staining microscopy
Definition
The microscopy of transparent objects that are in a mounting
medium, the Rl of which matches that of the object for a
certain wavelength, but which has a distinctly higher
dispersive power than the object. Under these conditions both
the object and the mounting medium appear coloured near
their interfaces. The colour with which the object appears is
distinctly different from that with which the mountant
appears. The colours and their differences depend on the
wavelength at which the Rls of the object and medium match
and the kind of microscopy used; dispersion staining may be
used in bright-field microscopy, the colour being concentrated
in the Becke line, in darkground microscopy or in phasecontrast microscopy
Term
Eyepiece (or ocular)
Definition
A lens system that is responsible for the angular
magnification of the final virtual image formed by it from the
primary image. This image is converted into a real image by
the observer’s eye or other converging lens system
Term
First-order red
Definition
Red, first-order (sensitive tint): the characteristic reddish violet
interference colour at approximately 530 nm retardation
Term
Focal plane
Definition
(1) A surface connecting all the points at which bundles of
parallel rays entering an ideal converging lens cross on the
other side of the lens, and thus containing a focal point; (2)
a surface at right angles to the optical axis of a lens (or
mirror) in which the image of an object lying at infinity is
formed: it is one of the cardinal planes
Term
Focusing eyepiece
Definition
An eyepiece with a mechanism for focusing an
(interchangeable) graticule or diaphragm mounted within it
and coinciding with the primary image.
Term
Iris
Definition
A diaphragm bounded by multiple leaves, usually metal,
arranged so as to provide an opening of variable size which
is adjustable by means of a control
Term
Interference colours
Definition
A mixed colour resulting from extinction or partial extinction caused by interference of one or several parts of a spectrum
Term
Köhler illumination
Definition
A method of illuminating objects in which an image of the source is projected by a collector into the plane of the aperture diaphragm in the front focal plane of the condenser. This latter, in turn, projects an image of an illuminated field diaphragm at the opening of the collector into the object plane
Term
Numerical aperture
Definition
A number (often symbolised by the letters NA) originally
defined by Abbé for objectives and condenser. It is given by
the expression ‘n × sin u’, where ‘n’ is the Rl of the medium between the lens and the object and ‘u’ is half the angular aperture of the lens.
Term
Objective
Definition
The first part of the imaging system, consisting of a lens, its mount, and any associated parts. It forms a primary image of the object.
Term
RI for Chrysotile
Definition
1.550
Term
RI for Chrysotile
Definition
1.550
Term
RI for Amosite
Definition
1.670
Term
RI for Anthophyllite
Definition
1.605
Term
RI for Tremolite
Definition
1.605
Term
Actinolite
Definition
1.640
Term
RI for Crocidolite
Definition
1.700
Term
relief
Definition
If a mineral has a refractive index that is much higher or lower than the surrounding materials, it will stand out relative to the surrounding material and will have thick or distinct
Term
why is blue slower
Definition
as it is a shorter wavelength than yellow
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