Name all of the Gram-positive cocci organisms that we have discussed in class.

(8)

• Staphylococcus aureus
• Staphylococcus epidermidis
• Staphylococcus saprophyticus
• Streptococcus pyogenes
• Streptococcus agalactieae
• Streptococcus pneumoniae
• Streptococcus mutans
• Enterococcus faecalis

Name the Gram-positive cocci organisms that are catalase positive.

(3)

All Staphylococcus spp.

• S. aureus
• S. epidermidis
• S. saprophyticus

Name the Gram-positive organisms that are catalase negative.

(5)

• Streptococcus spp.
• S. pneumoniae
• S. pyogenes
• S. agalactiae
• S. mutans
• Enterococcus faecalis

Name the Gram-positive cocci organisms that are catalase positive and mannitol fermentors.

(1)

Name the Gram-positive cocci organisms that are catalase positve and are not mannitol fermentors.

(2) 

• Staphylococcus epidermidis
• Staphylococcus saprophyticus

Name the Gram-positive rods that are spore forming.

(5)

• Clostridium spp.
• C. tetanus
• C. perfringens
• C. botulism
• C. difficile
• Bacillus anthracis

Name the Gram-positive cocci organisms that are catalase positive, do not ferment mannitol, and novabiocin resistant.

(1)

Staphylococcus saprophyticus

Name the Gram-positve cocci organisms that are catalase negative and alpha hemolytic.

(1)

Streptococcus pneumoniae

Name the Gram-positve cocci organisms that are catalase negative and beta hemolytic.

2)

• Streptococcus pyogenes (group A)
• Streptococcus agalactiae (group B)

Name the Gram-positve cocci organisms that are catalase negative and beta hemolytic and CAMP positive.

(1)

Name the Gram-positve cocci organisms that are catalase negative and beta hemolytic and CAMP negative.

(1)

Name the Gram-positve cocci organisms that are catalase negative and bile esculin positive (grow with esculin hydolysis).

(1)

Enterococcus faecalis

Name all of the Gram-positive rods that we have discussed in class.

(7)

• Corynebacterium diphtheriae
• Bacillus anthracis
• Clostridium perfringens
• Clostridium tetanus
• Clostridium botulism
• Clostidium difficile
• Micobacterium turerculosis

Name the Gram-positive rods organisms that are spore forming.

(5)

• Bacillus anthracis
• Clostridium spp.
• C. perfringens
• C. tetanus
• C. botulism
• C. difficile

Name the Gram-positive rods organisms that are spore forming and strict anaerobes.

(4)

• Clostridium spp.
• C. tetanus
• C. botulism
• C. perfringens
• C. difficile

Name the Gram-positive rods organisms that are not spore forming.

(2)

Corynebacterium diphtheriae

Mycobacterium tuberculosis

Name the Gram-positive rods organisms that are not spore forming and are catalase positive.

(1)

Name the Gram-positive rods organisms that are not spore forming and are acid fast.

(1)

Name the Gram-positive rods organisms that are not spore forming and is not acid fast.

(1)

 Name all of the Gram-negative rods or commata that we have discussed in class.

(9)

• Escherichia coli
• Klebsiella peumoniae
• Salmonella spp.
• S. enterica
• S. typhii
• Shigella spp.
• Pseudomonas aeruginosa
• Vibrio cholera
• Campylobacter jejuni
• Helicobacter pylori
• Aeromonas spp.

Name the Gram-negative rods that are oxidase positive.

(3)

• Pseudomonas aeruginosa
• Vibrio cholera
• Aeromonas spp.

• Vibrio cholera
• Aeromonas spp.

Name the Gram-negative rods that are oxidase positive, ferment glucose, and require Na⁺for growth.

(1)

Name the Gram-negative rods that are oxidase positive, ferment glucose, and do not grow in Na⁺ .

(1) 

Aeromonas spp.

Name the Gram-negative rods that are oxidase negative.

(5) 

• Escherichia coli
• Klebsiella peumoniae
• Salmonella spp.
• S. enterica
• S. typhii
• Shigella spp.

Name the Gram-negative rods that are oxidase negative and lactose positive.

(2) 

• Escherichia coli
• Klebsiella peumoniae

Name the Gram-negative rods that are oxidase negative, lactose positive, and indole positive.

(1)

Name the Gram-negative rods that are oxidase negative, lactose positive, and indole negative.

(1)

Name the Gram-negative rods that are oxidase negative, lactose positive, and indole negative.

(1)

Name the Gram-negative rods that are oxidase negative, lactose positive, indole negative, methyl red negative, and Vogues-Proskauer positive.

(1)

Name the Gram-negative rods that are oxidase negative, lactose positive, indole negative, methyl red positive, and Vogues-Proskauer negative.

(1)

Name the Gram-negative rods that are oxidase negative and lactose negative.

(3) 

• Salmonella spp.
• S. typhii
• S. enterica
• Shigella

Name the Gram-negative rods that are oxidase negative, lactose negative, and indole negative.

(2) 

• Salmonella enterica
• Shigella sonnei

Name the Gram-negative rods that are oxidase negative, lactose negative, indole negative, and urease negative.

(2)

• Shigella sonnei
• Salmonella enterica

Name the Gram-negative rods that are oxidase negative, lactose negative, indole negative, urease negative, and not motile.

(1)

Name the Gram-negative rods that are oxidase negative, lactose negative, indole negative, urease negative, motile, and produce H₂S.

(1)

Salmonella enterica

Name the Gram-negative rod that is oxidase positive, ferment glucose, and does not require Na₂ to grow.

(1)

Which Gram-negative organisms, that were discussed in class, are curved rods?

(2)

• Campylobacter jejuni
• Helicobacter pylori

C. jejuni - urease negative

H. pylori - urease positive

C. jejuni - nitrate reduction positive

C. pylori - nitrate reduction negative

Name all of the Gram-negative cocci/coccobacilli discussed in class.

(6)

• Neisseria gonorrhoeae
• Haemophilus influenza
• Bordetella pertussis
• Yersinia pestis
• Yersinia enterocolitica
• Legionella pneumophila

Nisseria gonorrhoeae

• Gram-negative diplococci
• non-motile
• no capsule
• capnophilic (grow under CO₂ conditions)
• fastidious; grows on chocolate agar

Disease - gonnorrhea (STD) 

Haemophilus influenzae

• Gram-negative rods/coccobacillary, small
• non-motile
• Fastidious, capnophilic (CO₂), faculative anaerobe
• grows on blood containing enriched media
• Requires both X-factor (hematin) and V-factor (NAD) for growth and will satellite (grow large colonies around other organisms that secrete V-factor i.e. S.aureus)

Disease - meningitis (capsular type b), osteomyelitis, epiglottitis, otitis; lives in the upper respiratory tract in humans; transmission is air-borne

Bordetella pertussis

• Gram-negative coccobacilli, small
• fastidious and slow growing
• will not grow on blood agar
• will grow on blood charcoal agar

Disease - whooping cough; transmission is air-borne

Yersinia pestis

• Gram-negative rods
• faculative anaerobe
• zoonotic
• grows best of blood and tissue media
• idendification by immunofluorescence

Disease - Bubonic plague from bite from rat fleas;

Yersinia enterocolitica

• Gram-negative rods
• zoonotic
• non-lactose fermenting
• urease positive
• oxidase negative
• faculative anaerobe
• grows best and is motile at 25°C (non-motile 37°C)

Disease - enterocolitis; from ingestion contaminated food and drink (raw milk, raw pork, etc.) Symptoms are fever, abdominal pain and diarrhea which may be watery and bloody.

• Gram-negative coccobacilli (in tissue and stain poorly, silver staining is better); pleomorphic on laboratory media
• fastidious; requires enriched media containing iron and cysteine w/absorbants to remove fatty acids
• diagnosis based on antibody detection (commonly) 

Disease: Legionnaires' disease; atypical pneumonia; Transmission: saprophyte inhalation of contaminated water from showers, air conditioning, cooling towers.

Name the spirochetes studied in class.

(2)

• Treponema palladium
• Borrelia burgdorferi

• coiled spirochetes
• can be seen with darkield illumination
• motile (flagella)
• cannot be grown in artificial media
• diagnosis is serological or by microscoic examinatin of the fluid from primary lesions

Disease: syphilis;  STD; primary=chancre/secondary/tertiary stages 

• spirochetes; visible by light microscopy
• microaerophilic
• complex nutritional requirements, long growth time
• diagnosis by serology or (bull's eye rash)
• zoonotic

Disease: Lyme disease with bull's eye rash and commonly, joint pains and fatigue ; untreated can cause neurologic and cardiac problems; Transmission is by deer tick bite; Treatment is with antibiotics

• no cell wall
• long thin like coccci but is pleomorphic

Disease: atypical pneumonia; Transmission is air-borne