Term
| What are the advantages of molecular methods for detection of specific microorganisms? |
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Definition
- Detect fastidious organisms
- Detect organisms that don't survive transport
- Much faster
- More sensitive and specific
- Can detect minute strain differences
- Less tech time
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Term
| What types of organisms can molcular methods be used to identify? |
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Definition
- Bacteria
- Viruses
- Parasites
- Fungi
- Yeasts
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Term
| What types of nucleic acids does molecular methods identify? |
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Definition
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Term
| What are the 3 methods of molecular detection? |
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Definition
- Hybridization of nucleic acid segments
- Nucleic acid amplification
- Signal amplification
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Term
| What is the principle of hybridization of nucleic acid segments? |
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Definition
based on ability of 2 complementary strands of nucleic acids to form hydrogen bonds to form a double helix
known strand = probe
unknown strand = target |
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Term
| What are 3 ways that hybridization of nucleic acid segments is detected? |
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Definition
- Radioactively labeled isotope
- Enzymatic
- Chemiluminescent (most sensitive)
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Term
| What are the 3 types of amplification as relating to molecular testing? |
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Definition
- The target
- The probe
- The probe signal
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Term
| What are the 3 specific types of molecular tests? |
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Definition
- PCR
- Nucleic acid probe
- Signal amplification
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Term
| What type of molecular test is PCR? |
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Definition
| Amplification (target NA is amplified) |
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Term
| What is another name for the nucleic acid probe molecular test? |
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Definition
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Term
| What is amplified in the nucleic acid probe molecular test? |
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Definition
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Term
| What is the principle of signal amplification as related to molecular testing? |
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Definition
| Increases the number of reporter molecules (can increase signal strength thousands of times) |
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Term
| How is strain typing and identification done? |
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Definition
- Different strain's DNA are digested with a restriction enzyme and the fragments are compared.
- Fragments called restriction fragment legnth polymorphisms (RFLP) patterns
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Term
| What does the GenProbe Aptima Combo 2 Assay test for? |
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Definition
- Neisseria gonorrhoeae
- Chlamydia trachomatis
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Term
| What kind of molecular test is the GenProbe Aptima Combo 2 Assay and what is the target? |
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Definition
- PCR test, amplification
- target is rRNA
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Term
| What is the principle of Clostridium difficile toxin B Cepheid Xpert? |
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Definition
| Detects presence of specific gene sequences which code for toxin B. |
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Term
| What is the principle behind the Shiga Toxin ST-1 and ST-2 by Immunocard Stat!EHEC test? |
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Definition
| Immunochromatographic test utilizing monoclonal antibodies labeled with red-colored gold particles |
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Term
| True or False. The Siga Toxin ST-1 and ST-2 by Immunocard Stat!EHEC test is a molecular test. |
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Definition
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