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Molecular Genetics Exam 3
Old exam questions
30
Biology
Undergraduate 4
04/18/2013

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Term
What is a merozygote?
Definition
partial diploids (zygotes) resulting from the interruption of conjugation
Term
The untranslated sequence at the 5' end of an mRNA molecule is called ________ sequence.
Definition
leader
Term
To what, other than the template strand of the gene from which it was transcribed, is the leader sequence complementary?
Definition
16s rRNA subunit
Term
What is the base sequence of the Pribnow box?
Definition
TATAAT
Term
What is the template base sequence which specifies the start codon?
Definition
TAC
Term
What is the template base sequence which specifies the chain termination codon?
Definition
ATT or (ATC)
Term
Name the protein which recognizes the codon UAG.
Definition
RF-1
Term
Name the protein which is involved in translocation.
Definition
EF-G
Term
Name the protein which recognizes the codon UGA.
Definition
RF-2
Term
Name the protein which breaks the bond between 3'-A of charged tRNA and its amino acid.
Definition
peptidyl transferase
Term
Name the protein that complexes with n-formylmet tRNA and GTP.
Definition
IF2
Term
Name the protein that complexes with GTP and incoming charge tRNA prior to its insertion into 50S subunit of ribosome.
Definition
EF-Tu
Term
Name the protein which is responsible for the formation of a peptide bond.
Definition
peptidyl transferase
Term
The general procedure for mapping new E. coli genes is to determine the approximate location of the gene by conjugation, using several Hfr strains, and then to determine the precise locatio of the gene by ___________ experiments.
Definition
cotransduction
Term
What proteins are found in the 50S subunit of bacterial ribosomes? (How many different proteins are present and what symbols are used to denote them?)
Definition
L1-L31
Term
The incorporation of donor DNA into the host cell chromosome during transformation results in the formation of a partial.
Definition
Term
The uptake of naked DNA by a bacterium is called ___________.
Definition
transformatino
Term
Bacteria containing integrated F plasmids are called _____________ strands.
Definition
Hfr
Term
In bacteria the site of RNA polymerase binding to the DNA template is called the ________ region of a gene.
Definition
promoter
Term
T or F: the only tRNA which never occupies the aminoacyl site in the 50S subunit of the ribosome during translation is n-formylmet tRNA.
Definition
T
Term
T or F: During amino acid activation, the amino acyl tRNA synthetase catalyzes the formation of an ester bond between the 3'terminal nucleotide of the uncharged tRNA and the amino group attached to the alpha-carbon of the amino acid.
Definition
F
Term
T or F: Transformation does not occur in E. coli cells except under very unusual conditions in the laboratory.
Definition
T
Term
In their transduction experiments, Zinder and Lederberg found that the cells of strain A Salmonella (donor cells) were lysogenic for phage P22.
Definition
F; used streptococcus
Term
Some kinds of viruses are incapable of bringing about generalized transduction due to the fact that during the infection cycle, the host cell DNA is completely degraded prior to virion assembly.
Definition
T
Term
T or F: When Griffith injected mice with a mixture of living Type II-R bacteria and heat killed type III-S bacteria, he recovered living type II-S bacteria from the bloodstreams of the deceased animals.
Definition
F: recovered type III-S
Term
T or F: Hotchkiss and Marmer foudn that double transformation occurs at appreciable rates only if both genes are extracted from the same donor cell.
Definition
T
Term
T or F: the Chain initiation codon is closer to the 5' end of mRNA than to the 3' end.
Definition
T
Term
In tRNA molecules which contain ribothymidylic acid, the conversion from uridylic acid is the result of the action of a(n) _____ enzyme.
Definition
mehtylating
Term
_________ is a nucleotide, found in some types of tRNA, wherein uracil is attached to ribose by a covalent bond between the no. 5 carbon of uracil and the no. 1 carbon of ribose.
Definition
pseudouridylate
Term
The presence of inosinic acid in tRNA molecules results from the post-transcriptional modification of ______.
Definition
adenine
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