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Molecular Genetics
Prokaryotic and Organellar genomes-Chapter 8
13
Biology
Graduate
03/02/2011

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Term
Prokaryotic Genomes
Definition
-E. coli genome is circular ds DNA
-isn't a chromosome
-found in a structure called a nucleoid
-some are linear
Term
supercoiling
Definition
occurs when additional turns are introduced into the double helix (positive) or turns are removed (negative)
-circular DNA responds by winding around itself for smaller spaces
Term
nucleoid structure
Definition
40 to 50 supercoils around the protein core
Term
plasmids
Definition
-not absolutely essential under normal conditions
-can be ultimately dispensed; but have much of their DNA in plasmid form
Term
E. Coli genome
Definition
packed with genes, less than 5 MB
Term
operon
Definition
group of genes involved in a singled biochemical pathway and expressed in conjunction with one another
-not all genes of an operon are related
Term
minimum number of genes necessary to maintain a living cell
Definition
-300 genes
-free living cell is capable of living on/in something
Term
vertical gene flow
Definition
from parent to child
Term
lateral or horizontal gene flow
Definition
transfer of genes between species, even between highly unrelated species
-happens by means of conjugation, transduction, and transformation
Term
Organellar genomes
Definition
-usually circular but some linear
-many copies per organelle, and many organelles per cell so you can have thousands of copies per cell
Term
how many genes are in organeller genome
Definition
-10-100 genes; majority for respiratory complex, tRNA, and rRNA
-chloroplast have about 200 genes
Term
endosymbiont theory
Definition
-bacteria invaded eukaryotic cells and lived as endosymbionts
-mitochondrial gene segments have been found in chloroplast genomes
-eventually lost most of their genes
Term
Why didn't all of the organeller genes get transferred to the nuclear genome
Definition
some proteins need to be translated in the organelle due to hydrophobic interactions that would be difficult in the cytoplasm
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