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Molecular Genetics
Viruses and Mobile Elements-Chapter 9
19
Biology
Graduate
03/02/2011

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Term
Virions
Definition
-Bacteriphage particles are pieces of DNA or RNA surrounded by protective proteins
-icosahedra have 20 sides and 12 vertices or variations
Term
filamentous virions
Definition
-have coat proteins coating the viral nucleic acid helix
-other coats protect a dsDNA core, attached to a proteinaceous tail (T4) or without tail fibers (lamda)
Term
virus genomes
Definition
-have dsDNA, ssDNA, dsRNA, and ssRNA
-can be linear or circular
-some are even segmented with one segment per one or two genes
-nonsegmented the whole viral genome serves as an operon
Term
overlapping genes
Definition
many small viruses have overlapping genes--gene within a gene in another reading from
-a few codons from one gene being shared in a different reading frame
Term
synchronous infection
Definition
-you can synchronously infect a batch of bacteria with a batch of virions and each stage of the infection cycle will occur at the same time with all the bacteria in the culture
-latent period has a steady number of infected cells, followed by a big increase in infected cells after the new virions are released to infect other cells--one-step growth curve
Term
lytic cycle
Definition
-host cells are starved and the viral sequence inserts itself into the host genome (a prophage) and is released
Term
enveloped virion
Definition
-surrouned by a lipid envelope with viral glycoproteins which recognizes a specific protein on the target host cell membrane
-others are "naked" with only protein capsid no envelope
Term
retroviruses
Definition
-have both RNA and DNA phases in their infection cycle
-After entry into the cell, the genome is copied into double stranded DNA by a few molecules of reverse transcriptase that the virus caries in its capsid
Term
provirus
Definition
-has LTRs (long terminal repeats) at each and three doing units in between
Term
LTR
Definition
-long terminal repeats
-three functions: promoter, enhancer, transcription terminator
-gag, pol and env are expressed as polyprotein that is cut into functional and structural proteins
Term
subviral pathogens
Definition
mostly found in plants
Term
satellite viruses
Definition
-satellite viruses are molecular parasites on plant viruses; code for their own coat protein but nothing else and are dependent upon their helper virus
Term
satellite RNAs
Definition
-Satellite RNAs don't even code for their own coat protein and are encapsulated with the helper virus coat protein
-also dependent upon the helper virus for replication
Term
Viroids
Definition
-smallest independent pathogen
-360 bp of circular ssRNA and depending on the host's ddRNA polymerase (transcriptase) for their replication
still need no helper virus
Term
virusoids
Definition
small circular ssRNA like viroids but are dependent on a helper virus for replication
-hepatitis delta virus
-rolling replication, producing a concatomer RNA
Term
prions
Definition
-coded for by one of our own genes
-mutated for PrPsc predisposes one to Creutzfeldt-Jakob disease
-power to induce other PrPsc or even PrPc proteins to take one that same perverted, pathogenic structure
Term
transposons
Definition
-mobile element which have a DNA intermediate as they transpose from one location (donor) to the other (recipient) in the genome
-if a copy is left behind it is "replicative"; if not then "conservative"
Term
Retroelements
Definition
-mobile elements which have an RNA intermediate as they transpose
-depend on normal cellular transcription to produce the RNA intermediate which is reverse transcribed by RT to produce dsDNA which is integrated by retroelement integrase into the recipient DNA
Term
Transposase
Definition
-can be carried by transposons, effect the integration of the transposon
-single transposon is caused in insertion sequence while a composite transposon comprises a gene
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