Term
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Definition
dsRNA look like viral RNA
response is to chop up the RNA; therefore, the mrna will not be produced |
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Term
| over expression of a gene |
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Definition
lean to an unnatural phenotype
powerful promoters are expressed only in the correct tissue type |
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Term
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Definition
| difficult, beginning with a gene of interest then later replacing the gene until the desired phenotype is produced |
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Term
| Site directed mutagenesis |
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Definition
-synthesize the oligonucleotide in order to create a new ssDNA circle
-50% of the clones wont have the mutation |
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Term
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Definition
| use a reporter gene that can be monitored such as lac Z, luciferase, or GFP |
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Term
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Definition
using an antibody to tag a specific protein; Ab is first conjugated to a marker and then fluoresces
-maker detection means target protein |
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Term
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Definition
| 30 percent of the genome is known, by comparitave genomics another 30 % was tentatively identified |
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Term
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Definition
| when the gene has not been assigned but it has been found in other organisms |
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Term
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Definition
-placing a lacZ reporter ORF (lacking its promoter) in frame in a transposon
-if transposon lands in a gene lac z will be expressed yielding blue colonies
-problem is screening all of the mutants |
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Term
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Definition
solves the problem with screening thousands of mutants; can create kanR knockouts
-has universal primers as well as unique sequences (barcode) which will identify each deletion mutant
-measures the growth rate of each clone by how well represented its barcode is in the PCR product |
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