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Molecular Genetics
DNA replication & protein synthesis
39
Biology
9th Grade
12/06/2013

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Term
What is the function of DNA?
Definition
stores genetic information
Term
Where is DNA located?
Definition
In the nucleus of eukaryotic organisms
Term
Where and when is DNA replicated?
Definition
During interphase in the nucleus
Term
What type of organic molecule is DNA?
Definition
nucleic acid
Term
What are the monomers of DNA called?
Definition
nucleotides
Term
Name the parts of a nucleotide.
Definition
deoxyribose sugar, phosphate group, & a nitrogenous base
Term
Name the 4 nitrogenous bases that make up DNA.
Definition
adenine, guanine, thymine, cytosine
Term
What are the base pairing rules for DNA?
Definition
adenine with thymine, cytosine with guanine
Term
Describe the shape of a DNA molecule.
Definition
double helix (like a twisted ladder)
Term
Who got credit for discovering the shape of DNA?
Definition
James Watson & Francis Crick
Term
What are the steps of DNA replication?
Definition
1. Helicase unwinds the DNA and break the hydrogen bonds between the bases
2. DNA Polymerase brings correct nucleotides to bind & proofreads
Term
What is the function of the enzyme DNA polymerase?
Definition
it brings nucleotides to bind during replication & ensures that no mistake were made
Term
What is the function of the enzyme DNA helicase?
Definition
It unwinds the DNA and breaks the hydrogen bonds between the base pairs
Term
What types of bonds hold the nitrogenous bases together?
Definition
hydrogen
Term
Give the complimentary DNA strand:
CCGAATTCATG
Definition
GGCTTAAGTAC
Term
How is RNA different from DNA?
Definition
1.RNA is made from ribose sugar, DNA is made from deoxyribose sugar
2. RNA is single stranded, DNA is double
3. RNA contains the base uracil instead of thymine
4. RNA can move outside of the nucleus, DNA cannot
Term
What is transcription and where does it occur?
Definition
DNA is copied into mRNA, happens in nucleus
Term
What is translation and where does it occur?
Definition
mRNA is converted into amino acids, happens at the ribosome
Term
What is the job of messenger RNA?
Definition
carries the coded instructions for how to make a protein from the DNA to the ribosome
Term
What is the job of transfer RNA?
Definition
carries specific amino acids to the ribosomes using the anticodon (that matches the codon on the mRNA)
Term
What is the job of ribosomal RNA?
Definition
it's part of the ribosome that holds mRNA in place while tRNA translates
Term
Give the complimentary mRNA strand to the following DNA strand:
CGCCTTACA
Definition
GCGGAAUGU
Term
What is a codon and where is it located?
Definition
3 bases, located on the mRNA
Term
What does a codon code for?
Definition
an amino acid
Term
What is an anticodon and where is it located.
Definition
Anticodon are complimentary to the codon, anticodons are located on the tRNA
Term
What does the codon AUG code for?
Definition
Met (the start amino acid)
Term
What does the codon AGU code for?
Definition
the amino acid Ser
Term
What does the codon UAA code for?
Definition
stop
Term
What is a mutation?
Definition
a change in the DNA sequence
Term
What is the difference between a point mutation and chromosomal mutation?
Definition
Point= one base is either changed, deleted or inserted
Chromosomal= entire chromosome is affected
Term
Does a point mutation always result in a different protein being made? Explain.
Definition
No, sometime a different codon codes for the same amino acid
ex: GGU, GGC, GGA and GGG all code for the amino acid Glycine
Term
What can cause mutations?
Definition
Errors during replication or crossing over, mutagens
Term
What are some examples of mutagens?
Definition
ultraviolet light from the sun, X rays, tobacco smoke
Term
Where are codons located?
Definition
On the mRNA
Term
Where are the anticodons located?
Definition
On the tRNA
Term
What types of bonds form between amino acids?
Definition
peptide bonds
Term
Why does DNA need to be replicated?
Definition
Before the cells divide, DNA needs to double so that each new cell has a complete set of DNA
Term

What is the following diagram showing?

[image]

Definition
a nucleotide of DNA
Term
Know the steps of protein synthesis
Definition
look in notes
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