Term
|
Definition
protein that activates DNA replication.
Active when bound to ATP |
|
|
Term
|
Definition
| introduction of foreign DNA into bacteria via plasmid |
|
|
Term
|
Definition
| introduction of foreign DNA into bacteria via virus |
|
|
Term
|
Definition
| shows which gene is in the middle |
|
|
Term
| restriction / modification systems |
|
Definition
innate bacterial immunity
2 enzymes: restriction endonuclease, modification methylase
ex: EcoRI |
|
|
Term
| Acquired bacteria immunity |
|
Definition
|
|
Term
|
Definition
only ecoli w/ R plasmid
Class II restriction modification system |
|
|
Term
|
Definition
Species gel - DNA
Probe - DNA or RNA |
|
|
Term
|
Definition
Species gel - RNA
Probe - DNA or RNA |
|
|
Term
|
Definition
Species gel - Protein A
Probe - Antibody for Protein A |
|
|
Term
|
Definition
Species gel - Protein A
Probe - Protein B with high affinity for Protein A |
|
|
Term
|
Definition
4.6 x 10^6 bp
84'
start leading strand synthesis |
|
|
Term
| PAS (Primeosome Assembly Site) |
|
Definition
lagging strand orgins
1-2000 bp apart
stem loop |
|
|
Term
|
Definition
DNA helicase
(dnaB, 52 kD, hexamer)
5'-> 3' donut surrounds lagging strand
In the replication complex but not part of DNAPIII subunit |
|
|
Term
|
Definition
removes positive super helical turns created by strand separation
not located with replication fork |
|
|
Term
|
Definition
single strand binding protein
stops ss-nucleases |
|
|
Term
|
Definition
(dnaG, DnaG, 52kD)
RNA polymerase contacts DnaB
in the replication complex but not part of DNAPIII subunit |
|
|
Term
|
Definition
| degrades primers in lagging strand |
|
|
Term
|
Definition
|
|
Term
|
Definition
dnaE, 3 copies, polymerase
part of the core subunits for DNAPIII |
|
|
Term
|
Definition
dnaQ, 3 copies, proofreading for correcting mismatches 3'->5' exo
part of the core subunits for DNAPIII |
|
|
Term
|
Definition
holE, 3 copies, stabilizes the core
part of the core subunits for DNAPIII |
|
|
Term
| core subunits for DNAPIII |
|
Definition
|
|
Term
|
Definition
| dnaN, 3 copies, processivity factor, "β-clamp", donut, 35 angstroms fits ds-DNA |
|
|
Term
|
Definition
dnaX, 3 copies, binds core & DnaB
part of the clamp loader dnaX-complex |
|
|
Term
|
Definition
| dnaX, 0* copies, may replace one Tau at times |
|
|
Term
|
Definition
| holA, 1 copy, opens beta clamp donut |
|
|
Term
|
Definition
| holB, 1 copy, holds beta clamp while gamma works |
|
|
Term
|
Definition
| holC, 1 copy, release primase |
|
|
Term
|
Definition
| holD, 1 copy, binds chi to tau |
|
|
Term
| clamp loader dnaX-complex |
|
Definition
| tau, gamma, delta, delta prime, chi, psi |
|
|
Term
|
Definition
A B C D X Y
B -> Eukaryotic replication
C -> Prokaryotic replication |
|
|
