Shared Flashcard Set

Details

Microscope Basics
Microscope Information
19
Microbiology
Not Applicable
10/10/2012

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Cards

Term
simple microscope
Definition
one lens
Term
compound microscope
Definition
two lens
Term
major parts of the microscope you use for sight
Definition
ocular (eyepiece) and objective (nosepiece)
Term
usual magnification of the ocular lens
Definition
10x
Term
Common magnifications of the objective?
Definition
4x, 10x, 45x, 100x
Term
What magnification do you use oil immersion for?
Definition
100x
Term
What is the total magnification?
Definition
Mag(objective) x Mag(ocular)
Term
What is the base structural part of the microscope that supports the basic frame?
Definition
arm (framework)
Term
What holds the slide?
Definition
stage
Term
Why do you use oil with an oil immersion lens?
Definition
oil prevents light loss due to diffraction
Term
As magnification of the objective lens increases, the working distance _______.
Definition
decreases
Term
What does a condenser do in BRIGHT FIELD MICROSCOPY?
Definition
directs light towards the objective lens
Term
What does a condenser do in DARK FIELD MICROSCOPY?
Definition
directs light at OBLIQUE angles away from the objective, causing objects to appear white on a dark field
Term
What is the purpose of the iris diaphragm?
Definition
it adjusts the diameter of the cone of light
Term

As you close down the diaphragm,

 

light intensity _______ (increases/decreases),

contrast _______ (improves, does not improve),

depth of field _________ (increases/decreases),

and it ________ (raises/lowers) resolution (with oil immersion lens).

Definition
decreases, improves, increases, limits
Term
Resolution equation?
Definition
d = lambda/2NA
Term

To improve resolution, _____ (lower/raise) D.

 

 

Definition
lower
Term

To improve resolution, (increase/decrease) lambda

 

Definition
decrease
Term
To improve resolution, (increase/decrease) the numerical aperture
Definition
increase
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