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Micronutrient Analysis
Blood analysis, urinalysis
32
Biology
Undergraduate 4
03/16/2010

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Term
Blood composition
Definition
plasma - 55%; blood cells - 45%
Term
Plasma composition
Definition
water, salts (electrolytes), plasma proteins (albumin, fibrinogen), hormones, nutrients (glucose, amino acids), waste
Term
Blood cell composition
Definition
red blood cells, white blood cells (granular & agranular), platelets
Term
Granulocytes (white blood cells)
Definition
(1) basophils - contain histamine granules (<1%)
(2) eosinophils - kill parasitic worms; inactivate antigen-antibody complexes (<5%)
(3) neutrophils- phagocytosis (50-70%)
Term
Agranulocytes
Definition
(1) lymphocytes - B lymph produce antibodies (25-35%)
(2) monocytes - phagocytosis as macrophages in tissue (3-9%)
Term
Differential blood cell count
Definition
measurement of the proportion of each WBC type present; expressed as a percentage
procedure: obtain a blood cell smear and count 100 WBCs; calculate percentage
Term
Reflotron
Definition
- measures urea, glucose, total cholesterol, HDL, uric acid, hemoglobin
- utilizes enzymatic reactions
- enzymatic changes detected by color changes in reflotron(R) reagent pads
- color changes detected by reflectance photometry controls provided
Term
Precinorm & Precipath
Definition
- precinorm should show results that are within the normal range
- precipath U should show results that slightly outside the normal range
- purpose for running these? to ensure that the Reflotron(R) is working appropriately and that it will be able to detect readings that are both within and outside the normal range
Term
Reflotron(R) - BUN
Definition
- measure of kidney function; urea made by the liver and cleared by the kidneys
- test strip contains (1) urease, (2) pH indicator
- urease converts urea --> ammonia
- ammonia is detected!
Term
Reflotron(R) - Glucose
Definition
- test strip contains (1)glucose oxidase, (2) color indicator
- glucose (via glucose oxidase) --> gluconic acid + H2O2
- H2O2 detected by color indicator!
Term
Reflotron(R) - Total Cholesterol
Definition
- test strip contains (1)cholesterol esterase, (2) cholesterol oxidase, (3) color indicator
- cholesterol esters (via CE) --> cholesterol (via CO) --> H2O2
- H2O2 detected by color indicator!
Term
Reflotron(R) - HDL
Definition
- test strip contains (1) dextran sulfate & magnesium acetate (to ppt VLDL and LDL), (2) cholesterol esterase, (3) cholesterol oxidase, (4) color indicator
- HDL is oxidized to H2O2, which is detected by the color indicator
Term
Reflotron(R) - TG
Definition
- test strip contains enzymes that breakdown TG into fatty acids and glycerol-3-phosphate
- also, enzymes that convert G3P --> H2O2
Term
Reflotron(R) - Uric Acid
Definition
- measure of purine breakdown because uric acid is the final product (humans contain uricase, but it is not functional)
- test strip contains (1) uricase, (2) peroxidase, (3) color indicator
- uric acid (via uricase) --> H2O2
Term
Reflotron(R) - Hemoglobin
Definition
- test strip contains (1) enzymes that promote hemolysis and Hb oxidation, (2) color indicator
- oxidized Hb complexes with another compound and forms a highly colored molecule
Term
Reflotron(R) - Hemoglobin
Definition
- test strip contains (1) enzymes that promote hemolysis and Hb oxidation, (2) color indicator
- oxidized Hb complexes with another compound and forms a highly colored molecule
Term
Reflotron Normal Values
Definition
- BUN: 5-23 mg/dL
- TC: <200 mg/dL desirable, >240 high
- HDL: >35(M), >45(W)
- TG: 40-160(M), 35-135(W)
- LDL: <135 desirable
- glucose: fasting 76-110 mg/dL
- Hb: 14-18 (M), 12-16 (F)
- uric acid: <7 (M), <5.7 (F)
Term
Urine
Definition
composition: water, salts, organic material, waste, urea, uric acid, K+, Cl-
Term
Uses of urinalysis
Definition
can be used to detect (1) presence of drugs, (2) pregnancy (HCG), (3) UTI, (4) DM, (5) PKU
Term
Urinalysis - presence of glucose
Definition
- detected by adding Benedict's reagent to urine sample + boiling for 2 m
- Benedict's reagent tests for presence of reducing sugars --> contains copper (II) sulfate, which is reduced to copper (I) oxide
Term
Urinalysis - presence of protein (albumin)
Definition
- detected by adding Biuret's reagent
- Biuret's reagent (normally blue), turns purple in the presence of peptide bonds
Term
Urinalysis - presence of yeast
Definition
- detected by adding 3% H2O2 to a sample of urine
Term
Salivary Amylase
Definition
- Starch (via amylase) --> maltose
- Lugol's iodine - for presence of starch; if present, blue-black color
-Benedict's reagent - for maltose (2 glu mol); if present, orange color
Term
Lipase
Definition
fat --> fatty acids + glycerol
Term
Proteases
Definition
- hydrolase
- breaks peptide bonds
- protein --> amino acids
Term
Pepsinogen --> pepsin
Definition
- pepsinogen (zymogen) activated by HCl
- pepsin is a protease
Term
Trypsin
Definition
- protease in small intestine
Term
Beano
Definition
- reduces flatulence
- contains alpha galactosidase, which cleaves complex sugars into simpler sugars, which aids in their absorption
- if undigested, pass into large intestine where flora ferment sugars (produce gas as byproduct)
Term
Lactase
Definition
- lactose --> glucose + galactose
Term
BMI
Definition
underweight: <18.5
normal: 18.5-24.9
overweight: 25-29.9
obese: >30
Term
Children and BMI
Definition
Underweight: <5th percentile
Normal: 5th - 85th percentile
At risk of being overweight: 85th - 95th percentile
Overweight: >95th percentile
Term
Body fat
Definition
Men: min=5%, avg=15-18%, too much=>25%
Women: min=12%, avg=22-25%, too much=>32%
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