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Microbiology- Laboratory Diagnosis of Enterobacteriaceae
Test 2
33
Biology
Undergraduate 4
09/23/2009

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Term
Which Enterobacteriaceae species tends to be bipolar?
Definition
Yersinia pestis
Term
Which two Enterobacteriaceae genera are always nonmotile?
Definition
Klebsiella and Shigella
Term
Which genus of Enterobacteriaceae is nonmotile at room temperature?
Definition
Yersinia
Term
Describe the oxygen requirements of Enterobacteriaceae.
Definition
facultative anaerobes
Term
Describe the nutritional requirements of Enterobacteriaceae.
Definition
non-fastidious
Term
Which genus of Enterobacteriaceae produces pigments?
Definition
Serratia
Term
Which species of Enterobacteriaceae may produce a beta hemolysin?
Definition
E. coli
Term
Which genus of Enterobacteriaceae swarms on BAP and other moist media?
Definition
Proteus
Term
What inhibits the swarming of Proteus?
Definition
increasing concentration of agar from 1.5% to 5%
Term
What does the Carbohydrate utilization test test for?
Definition
fermentation of glucose, lactose, maltose and sucrose
Term
What test differentiates between late lactose fermenters and non-lactose fermenters?
Definition
orthonitrophenylgalactoside
Term
What enzyme is detected by the ONPG test?
Definition
beta galactosidase
Term
What four tests are included in IMViC?
Definition
Indole, Methyl red, Vogues-Proskauer and Citrate
Term
What test detects tryptophanase?
Definition
indole
Term
Which test is performed in tryptone broth?
Definition
indole
Term
Which test uses Kovac's or Ehrlick's reagent?
Definition
indole
Term
What does methyl red detect?
Definition
strong acids. glucose fermentation?
Term
What three sugars are present in TSIA?
Definition
glucose, lactose, sucrose
Term
Which genera are Oxidase positive and carbohydrate fermentor positive?
Definition
Vibrio, Aeromonas, Pleisiomonas
Term
Which genus is oxidase positive and carbohydrate fermentor negative?
Definition
Pseudomonas
Term
If you did an oxidase test and it was negative, what would you do next?
Definition
Lactose fermentation/ MacConkey agar
Term
Oxidase negative, negative on MacConkey agar, nonmotile.
Definition
Shigella
Term
Does Shigella ferment lactose?
Definition
No.
Term
Is E.coli oxidase positive or negative?
Definition
negative
Term
Oxidase negative, positive on MacConkey agar, indole positive.
Definition
E.coli
Term
Which four genera are oxidase positive?
Definition
vibrio, aeromonas, pleisiomonas, pseudomonas
Term
Oxidase negative, positive on macconkey agar, negative for indole, negative for urease production.
Definition
enterobacter aerogenes
Term
Oxidase negative, positive on macconkey agar, negative for indole, positive for urease production.
Definition
Klebsiella pneumoniae
Term
Is Klebsiella pneumoniae a lactose fermenter?
Definition
yes.
Term
What two genera produce H2S?
Definition
Proteus, Salmonella
Term
Is Proteus vulgaris indole positive or negative?
Definition
positive
Term
Is Proteus mirabilis indole positive or negative?
Definition
negative
Term
Which organism produces H2S and is citrate positive?
Definition
Serratia marcesens
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