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Microbiology
Microbiology flash cards, Exam 2 Dr Muller Spring '09
15
Biology
Undergraduate 3
04/21/2009

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Cards

Term
How are milk proteins denatured?
Definition
They are denatured by the acids produced during microbial fermentative metabolism
Term
The two major bacteria in the starter culture of yogurt
Definition
Streptococcus thermophilus (responsible for acid) and lactobacillus bulgaricus (responsible for flavor)
Term
How is the curd made?
Definition
Milk proteins coagulate due to lactic acid fermentation?
Term
How is cheese made?
Definition
Curds are formed, curd is pressed so water is removed then salted, product then may be inoculated one or two times depending on the variety
Term
How is butter made?
Definition
Churning milk causes fat globulins to aggregate and seperate from the watery buttermilk
Term
Yeast grows under what type of conditions?
Definition
Aerobic whch results in increased CO2 production and some EtOH accumulation
Term
What is special about Baker's Yeast?
Definition
Selected strains of saccharomyces cervisiae produce enzymes that hydrolyze the starch and carbohydrates found in the grains used for baking
Term
How is sauerkraut made?
Definition

Cabbage is shredded and wilted with addition of NaCL

Brine which forms lactic acid bacteria and restricts growth of gram negative bacteria

two microbes responsible for production are Leuconostoc mesenteriodes (drops pH to 3.5) and Lactobacillus plantarum (dominant fermenter)

Term
How do you make wine?
Definition

juice- white wine the seeds and skin is taken out

this is the must and is pasteurized

it ferments for 3-5 days at 20-28* C final product contains 10-18% EtOH

Sediments removed by racking

 

Term
How do you make beer?
Definition

malting- Grains are mixed with water and allowed to germinate for one week, then crushed to release enzymes

mashing- the enzymes in the malt hydrolyze the starches and proteins when incubated at 70*C

mash is heated with hops, solid settles

the clear liquid is called the wort and is heated to pitched- fully inactivate the hydrolytic enzymes and inoculated with yeast

then fermented

Term
the major effect of temperature
Definition
affect the velocity of enzyme catalyzed reactions
Term
What happens when milk gets pastuerized?
Definition
nucleic acids and proteins get denatured and membrane lipids melt
Term
Common milkborne pathogens
Definition

mycobacterium tuberculosis, salmonella, strep and brucella

NOTE THESE DO NOT FORM SPORES

Term
What is the only way to achieve heat-sterilization?
Definition
Steam sterilization, in an autoclave
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