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Microbiology
Test 2
77
Biology
Undergraduate 3
02/16/2009

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Cards

Term
Streak Plate Technique
Definition
Microbiologists use the _____ to isolate pure cultures of bacteria
Term
Facultative Anaerobe
Definition
A bacterium that can respire with oxygen when it is available, but can use fermentation if oxygen is absent
Term
Organoheterotrophs
Definition
Organisms that obtain their carbon and energy from existing organic compounds
Term
Acidic
Definition
Oranges have a pH of 5. A scientist isolates several types of bacteria that cause spoilage of orange juice concentrates. Which growth condition would be most appropriate to maintain these bacteria in culture?
Term
Plate count method
Definition
Method to measure the concentration of viable cells by determining the number of colonies that arise from a sample added to an agar plate
Term

many organisms can degrade gelatin

gelatin may not remain solid at temperatures required for microbial growth

gelatin is not stable during sterilization procedures

gelatin liquefies at warm temperatures.

Definition
The problem with using gelatin as a solidifying agent for microbiological media is that...
Term
Psychrophile
Definition
A microorganism isolated from the Antarctic would most likely be...
Term
Catalase and superoxide dismutase
Definition
Enzyme(s) that help organisms withstand the toxic byproducts associated with diatomic oxygen is...
Term
A differential medium
Definition
A growth medium that can discriminate between two different bacterial taxa based on an observed change in the medium is...
Term
Exponential (log) phase
Definition

Stage of growth in which cells divide at a constant rate

 

Generation time is measured during this time of active multiplication

Term
lag
Definition
The growth phase in which cells are adapting to new growth conditions is termed...
Term
Distinguish one group of organisms from others
Definition
Differential media is used to...
Term
Chemostat
Definition
Maintains a bacterial culture in continuous exponential growth
Term
Osmotolerant prokaryotes
Definition
An organism that has an advantage in water as the water evaporates...
Term
Membrane filtration
Definition
The Most Probable Number (MPN) method of determining cell numbers is based on...
Term
Stationary
Definition
During which phase of culture growth are the number of newly formed cells approximately equal to the number of dying cells?
Term
Biofilm
Definition
Polysaccharide-encased community of microorganisms
Term
Chemically Defined Medium
Definition

Bacterial growth medium composed of precise mixtures of known pure chemicals

 

Generally used for specific experiments when nutrients must be precisely controlled

Term
Complex Medium
Definition
Bacteriological medium that contains protein digests, extracts, or other ingredients that vary in their chemical composition
Term
Differential Medium
Definition

Bacteriological medium that contains an ingredient that can be changed by certain bacteria in a recognizable way

 

Used to differentiate organisms based on their metabolic traits

Term
Generation time
Definition
The time it takes for a population to double in number
Term
Obligate Aerobe
Definition
Organism that requires molecular oxygen
Term
Pure culture
Definition
A population of organisms descended from a single cell and therefore separated from all other species
Term
Selective Medium
Definition
Bacteriological medium to which additional ingredients have been added that inhibit the growth of many organisms other than the one being sought
Term
Obligate Anaerobe
Definition
Organism that cannot multiply and is often killed in the prescence of oxygen
Term
Cross Septum
Definition
Structure that divides the bacterial cells in two during binary fission
Term
12 hours
Definition
The generation time of mycobacterium tuberculosis is...
Term
GTP
Definition
FtsZ proteins derive their energy from the breakdown of
Term
FtsZ
Definition
____ forms a contractile ring at the cell division site
Term
False
Definition

The cross septum starts to form at the beginning of DNA replication

 

T/F

Term
Replisomes
Definition
DNA replication occurs on ____
Term
False
Definition

Bacteria growing in log phase are least affected by antiobiotics

 

T/F

Term
Antiseptic
Definition
A disinfectant that is non-toxic enough to be used on skin
Term
Aseptic Technique
Definition
Procedures that minimize the chance of unwanted microbes being accidentally introduced
Term
Bactericidal
Definition
Kills bacteria
Term
Bacteriostatic
Definition
Prevents the growth of, but does not kill, bacteria
Term
Degerm
Definition
Treatment used to decrease the number of microbes in an area, usually skin
Term
Disinfectant
Definition
A chemical used to destroy many microorganisms and viruses
Term
Germicide
Definition
Kills microorganisms and viruses
Term
Pasteurization
Definition

 A treatment, usually brief heating, used to reduce the number of spoilage organisms and to kill disease-causing microbes

Term
Preservation
Definition
The process of inhibiting the growth of microorganisms in products to delay spoilage
Term
Sterilant
Definition
A chemical used to destroy all microorganisms and viruses in a product, rendering it sterile
Term
Sterile
Definition
Completely free of all microbes
Term
Sterilization
Definition
The process of destroying or removing all microorganisms and viruses through physical or chemical means
Term
Autoclaving
Definition
Medical wastes, laboratory equipment, and microbiological media are sterilized through the process of...
Term
True
Definition

In feedback inhibition, the inhibitor of the biochemical pathway is typically the final product of the biochemical pathway.

 

T/F

Term
Allosteric Site
Definition
What does the inhibitor bind to during feedback inhibition?
Term
False
Definition

If the end product of a biochemical pathway becomes available in the environment, it is crucial for the cell to continue making the product.

 

T/F

Term
True
Definition

A biochemical pathway consists of a specific sequence of enzyme-catalyzed reactions.

 

T/F

Term
Energy ONLY
Definition
Cyclic photophosphorylation produces...
Term
Water
Definition
In noncyclic photophosphorylation, the electrons passed down through the electron transport system are obtained from...
Term

photosystem II

ATP production

photosystem I

NADPH production

Definition
What is the correct sequence in noncyclic photophosphorylation?
Term
True
Definition

Cyclic photophosphorylation involves a single photosystem.

 

T/F

Term
True
Definition

Oxygen is produced in noncyclic photophosphorylation but not in cyclic photophosphorylation.

 

T/F

Term
True
Definition

Bacterial photosynthesis uses only one photosystem

 

T/F

Term
True
Definition

Bacterial photosynthesis does not produce oxygen

 

T/F

Term
True
Definition

The photosynthetic bacterium Rhodobacter sphaeroides uses bacteriopheophytin and ubiquinone as electron carriers.

 

T/F

Term
False
Definition

In reverse electron flow, ATP hydrolysis provides the energy to convert NADH to NAD+

 

 T/F

Term
Chlorobium
Definition
___ uses H2S as an electron donor forming elemental sulfur as a byproduct.
Term
Stroma
Definition
The Calvin Cycle takes place in the ________ of chloroplasts.
Term
ATP and NADPH
Definition
The Calvin Cycle uses ___ and ___, which are products of the light reactions of photosynthesis.
Term
RuBP
Definition
The Calvin cycle begins by the attachment of CO2 to___
Term
True
Definition

Glyceraldehyde-3-phosphate and 3-phosphoglycerate are three-carbon molecules produced during the Calvin Cycle.

 

T/F

Term
True
Definition

The Calvin Cycle functions to construct carbohydrates from carbon dioxide.

 

T/F

Term
ATP
Definition

The energy currency of cells

Hydrolysis of its unstable phosphate bonds can be used to power endergonic reactions

Term
Anabolism
Definition

Processes that utilize energy stored in ATP to synthesize and assemble the subunits (building blocks) of macromolecules that make up the cell

Biosynthesis

Term
Catabolism
Definition
Processes that harvest energy released during the breakdown of compounds such as glucose, using it to synthesize ATP
Term
Electron Transport Chain
Definition
Group of membrane-embedded electron carriers that pass electrons from one to another, and, in the process, move protons across the membrane to create a proton motive force
Term
Enzyme
Definition
A protein that functions as a catalyst, speeding up a biological reaction
Term
Fermentation
Definition
Metabolic process that stops short of oxidizing glucose or other organic compounds completely, using an organic intermediate such as pyruvate or a derivative as a terminal electron acceptor.
Term
Oxidative Phosphorylation
Definition
Synthesis of ATP using the energy of a proton motive force created by harvesting chemical energy
Term
Photophosphorylation
Definition
Synthesis of ATP using the energy of a proton motive force created by harvesting radiant energy
Term
Precursor Metabolites
Definition
Metabolic intermediates that can either be used to make the subunits of macromolecules, or be oxidized to generate ATP
Term
Proton motive force
Definition
Form of energy generated as an electron transport chain moves protons across a membrane, creating a chemiosmotic gradient
Term
Respiration
Definition
Process that involves transfer of electrons stripped from a chemical energy source to an electron transport chain, generating a proton motive force that is then used to synthesize ATP
Term
Substrate-Level Phosphorylation
Definition
Synthesis of ATP using the energy released in an exergonic (energy-releasing) chemical reaction
Term
Terminal Electron Acceptor
Definition
Chemical such as O2 that is ultimately reduced as a consequence of fermentation or respiration
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