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Microbiology
Chapter 3
29
Biology
Not Applicable
02/10/2009

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Term
wavelength
Definition
feature of light that describes the distance between two successive waves
Term
resolution
Definition
ability to distinquish fine detail
Term
refraction
Definition
feature of light that describes its path change as it moves from one medium to another; bending of light
Term
magnification
Definition
the number of times larger an image is compared to the object
Term
compound light microscope
Definition
two magnifying lenses
Term
condenser
Definition
lens system below the stage that focuses light onto the specimen
Term
light source
Definition
light bulb at the base of the microscope
Term
diaphragm lever
Definition
controlling extensions from the condenser system to increase or decrease the amount of light reaching the specimen
Term
objective lens
Definition
magnifying lens coming out of the nose piece
Term
ocular lens
Definition
magnifying lens near where the eyes are placed
Term
darkfield
Definition
blocks direct light, unstained cells easily seen; a live bacteria cell
Term
brightfield
Definition
large cells easily seen but small cells difficult to see w/o stain; a Gram stained bacterial cell
Term
TEM
Definition
greatest magnification and resolution; intracellular detail of a bacterial cell
Term
phase contrast
Definition
partially blocks light allowing for direct and diffracted light; intracellular detail of an unstained eukaryote cell
Term
fluorescent
Definition
UV strikes specimen and is converted to visible light; sputum stained with auramine O
Term
basic dye
Definition
positively charged, easily adheres to most cells; includes both dyes in the Gram stain; has a color associated with it
Term
smear
Definition
a monolayer spread of cells on a microscope slide
Term
fix
Definition
apply heat to cells to cells to make them adhere to the glass slide
Term
mordant
Definition
additive that enhances stain property
Term
stain
Definition
colored agent to allow better visualization
Term
decolorizer
Definition
agent to remove stain
Term
refraction index
Definition
The relative velocity with which light passes through a substance; each medium has a refractive property in comparison to air; air = 1.o
Term
electron microscope
Definition
electrons coming off a heated metal move as waves but with much smaller wavelengths than light, this allows greater resolution
Term
SEM
Definition
exterior morphology revealed
Term
differential stain
Definition
A stain that distinguishes objects on the basis of reactions to the staining procedure
Term
counterstain
Definition
A second stain applied to a smear, provides contrast to the primary stain
Term
acid fast stain
Definition
A differential stain used to identify bacteria that are not decolorized by acid-alcohol
Term
gram stain
Definition
A differential stain that classifies bacteria into two groups, gram-positive and gram-negative
Term
capsule stain
Definition
Capsule staining provides a contrasting background, so the capsules show up as light areas surrounding the stained cells.
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