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In general, individual viruses are physically larger than individul bacteria |
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All bacteria have capsules |
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All bacteria have a cell wall |
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Spore formation allows certain bacteria to survive under unfavorable conditions. |
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A micron, or micrometer, is one thousandth of a millimeter. |
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| Low environmental temperatures are detrimental for bacteria because they denature bacterial proteins. |
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The optimum pH range for most bacteria is about the same as the body pH range for people and animals. |
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Bacterial growth in a broth tube that is clumpy is described as flocculent. |
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Every veterinary hospital must be prepared to do all of their own microbiology work. |
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It is ok to autoclave surgery packs and old micro cultures at the same time. |
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Aseptic technique is absolutely required for proper microbiological culturing. |
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It is possible for lab technicians to infect themselves through improper handling of microbiological samples. |
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The best time to collect culture specimen's in on the day after meds have been started. |
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Before transferring a broth sample to a new tube or culture plate, you should mix the broth culture by vigorously shaking it. |
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When sterilizing a wire loop, you should put the loop end directly into the flame first. |
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You should remove and hold the cap of the broth tube with your pinkie finger while you are working with a tube. |
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Broth tube lids should be sterilized both before and after the inoculating loop has been inserted. |
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| Bacterial cultures is petri dishes should be incubated lid down, agar up. |
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T/F
When gram staining bacterial smears on slides, you should squirt water under pressure directly onto the smears in order to wash them thoroughly. |
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Definition
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| Spherical bacterial cells are bacilli |
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Definition
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| After gram staining a smear on a slide, the slide should be wiped dry. |
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T/F
Dextrose and glucose are two completely different things. |
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A synthetic culture medium is one prepared from pure chemical substances. |
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Chocolate agar does not really have any chocolate in it. |
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Blood agar is made from rabbit blood. |
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Blood agar is considered to be a differential type of media. |
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Catalase and coagulase tests are used to further differentiate gram negative bacteria. |
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The catalase and coagulase tests both test for enzymes that are part of the bacterial defense mechanism. |
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The purpse of the coagulase test is to distinguish between Staph species. |
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Definition
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Both Staph and Strep are common commensal organisms |
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Enterobacteriaceae are gram negative intestinal bacterial rods that can ferment glucose. |
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Definition
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Enterobacteriaceae are subdivided into colliforms and non-colliforms. |
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Definition
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Term
| MacConkey agar contains lactose so it can differentiate among lactose and non-lactose fermenters. |
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Definition
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T/F
Lactose fermenters such as E. coli grow as pink colonies and turn MacConkey agar yellow. |
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TSI agar stands for triple salt iron agar. |
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When incubating inoculated agar tubes the lid should be tightly closed. |
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The enterotube test is run before the oxidase test. |
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Definition
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The purpose of inoculating an unknown bacteria into a motility tube is to test for the presence of flagella. |
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Definition
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A motility tube should be inoculated with a loop. |
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Definition
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All of the compartments of an enterotube and oxiform tube are run under anaerobic conditions. |
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Definition
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Enterotube and oxiform tube biocodes can be looked up in the same book. |
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Definition
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T/F
The hanging drop method is used to test for bacterial gas production. |
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Definition
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T/F
The purpose of antibiotic sensitivity testing is used ti select the drug that a particular strain of bacteria is susceptible to. |
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Definition
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All antimicrobial drugs diffuse through agar at the same rate. |
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Definition
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T/F
The isolation streak technique is used to apply bacteria to the surface of the agar in the disc diffusion antibiotic sensitivity test. |
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Definition
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T/F
Bullseye plates are commercial combination plates which are used for both bacterial ID and antimicrobial sensitivity testing. |
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Definition
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Term
| Mycology is the study of? |
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Definition
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Term
| Are bacteria eukaryotic or prokaryotic? |
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Definition
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Term
| What is the movement in response to light? |
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Definition
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Term
| can flagella be seen by a light microscope? |
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Definition
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Term
| What is the main function of pili? |
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Definition
| make the bacterium adhere to other bacteria and other objects |
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Term
| What is a paliside arrangement? |
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Definition
| chinese arrangement of rod shaped bacteria |
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Term
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Definition
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Term
| What type of solution causes bacteria to lyse? |
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Definition
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Term
| how do bacteria reproduce? |
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Definition
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Term
| Where is the capsule of a bacterium located? |
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Definition
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Term
| what is the function of a bacterial capsule? |
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Definition
| protect against phagocytosis, promote attachment, and prevent dehydration |
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Term
| what is the function of a bacterial cell wall? |
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Definition
| provide rigidity and maintain size and shape |
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Term
| what structure is found just inside the bacterial cell wall? |
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Definition
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Term
| what is the function of ribosomes? |
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Definition
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Term
| what is peritrichous flagellation? |
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Definition
| flagella all around the bacterium |
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Term
| what transfers dna from one bacterium to another? |
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Definition
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Term
| do endospores stain easily? |
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Definition
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Term
| The most pathogenic bacteria are? |
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Definition
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Term
| under favorable conditions how long does it take a newly formed bacteria to reach adult size and divide? |
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Definition
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Term
| what are the four stages of bacterial growth in media? |
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Definition
| lag, logarithmic, stationary, decline |
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Term
| when is the best time to evaluate and identify bacteria growing in media? |
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Definition
| When bacteria is doubling at regular intervals |
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Term
T/F
In general, basic culture media should be isotonic to bacteria |
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Definition
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T/F
Gelatin culture media are liquid at the incubator temperature. |
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Definition
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T/F
Bacteria can use gelatin for nutrients |
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Definition
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T/F
Selective media kills or inhibits all but few bacteria |
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Definition
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Term
| What type of agar deals with hemolysis? |
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Definition
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Term
| what agar contains phenol red as a pH indicator? |
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Definition
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Term
| is an organism causing hemolysis pathogenic or non-pathogenic? |
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Definition
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Term
| mannitol salt agar is selective for what type of bacteria? |
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Definition
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Term
| what type of staph is most likely to turn mannitol salt agar yellow? |
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Definition
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Term
| catalse tests should be run on bacteria grown on what type of agar? |
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Definition
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Term
| whats more accurate, the tube or slide coagulase test? |
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Definition
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Term
| what kind of plasma does the coagulase test use? |
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Definition
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Term
| is s. epidermis or s. aureus coagulase positive? |
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Definition
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Term
| what bacteria is most likely to be found in an abscess? |
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Definition
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Term
| what is a fastidious organism? |
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Definition
| requires very specific conditions to grow |
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Term
| agar is a dried extract of what? |
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Definition
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Term
| what substance is used as the reagent in a catalase test? |
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Definition
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Term
| what are the bubbles composed of in a catalase test? |
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Definition
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Term
| when is a coagulase test performed? |
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Definition
| on a gram positive catalase positive bacteria |
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Term
| what is a commensal microscopic organism? |
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Definition
| derives slight benifit from its host without causing much harm |
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Term
| anthrax is caused by a type of what? |
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Definition
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Definition
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Definition
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Definition
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Definition
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Definition
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| what causes equine strangles? |
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Definition
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Term
| what causes circling disease in ruminants? |
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Definition
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Term
| what causes the diamond skin disease of swine? |
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Definition
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Term
| what causes botulism and tetanus? |
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Definition
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Term
| colliforms can ferment what? |
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Definition
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Term
| is e. coli a coliform or non-coliform? |
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Definition
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Term
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Definition
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Term
| what causes snuffles in rabbits? |
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Definition
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Term
| wha causes ear infections in dogs? |
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Definition
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Term
| what causes wooden tongue in cattle? |
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Definition
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Term
| what causes pink eye in cattle? |
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Definition
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Term
| what causes scours in calves? |
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Definition
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Term
| what causes shipping fever in cattle? |
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Definition
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