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Microbiology
Gram Negative Rods
9
Science
Graduate
05/08/2008

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Cards

Term
Enterobacteriaceae
Definition

Gram Negative

 

Facultative organisms

 

Glucose fermenters

 

Oxidase negative

 

capsular antigens

 

can be found in human/animal GI, but widely distributed  

Term
Effects of endotoxin
Definition
IL-1 release: fever
 
TNF release: septic shock mediator
 
Nitroic oxide release: vasodilation, hypotention
 
Hageman factor: activation of clotting (DIC disseminated intravascular coagulation)
 
Complent - alternative pathway 
Term
E. coli
Definition
Contains K-1 capsule
 
opportunistics - infects ulcers in GI
 
lactose +
EMB agar shows green sheen
 
Clinical manifestations: UTI, neonatal meningitis/sepsis, nosocomial infections, enterocolitis 
Term
Klebsiella
Definition
fecal-oral orfecal-vaginal transmission
 
opportunistic
 
lactose +, large capsule (mucoid colonies)
 
Clinical manifestations: community acquired pneumonia, nosocomial infections, UTI 
 
Term
Proteus
Definition
Opportunistic pathogen
 
lactose -, swarming growth, urease +
 
Clincal manifestations: UTI - stone formation, nosocomial infectgion - septicemia
Term
Serretia marcescens
Definition
found in GI tract/soil/ water
 
widely found in hospitals/environment
 
Produces red pignment, very antibiotic resistant
 
Clinical manifestations: nosocomial pathogen 
Term
Pseudomonas aerunginosa
Definition
warm water sources, some in GI tract
 
opportunistic
 
oxidase +, non-glucose fermenter, blue-green pigment (pyocyanin)
 
very antibiotic resistant: use 3rd generation AMG
 
Clinical manifestations: wound infections of burn pts, nosocomial infections (UTI/sepsis), pneumonia (cystic fibrosis), external ear infection, hot tub folliculitis 
Term
LAL preparation
Definition

Ameobocytes will clot in the presence of minute amount of endotoxins

 

Tag of these ameobocytes are placed with the autoclave to ensure no endotoxins are present 

Term
In preventing disease with enteric GNR, we can use _____________ or _____________
Definition

Filtration, LAL preparations

 

Filtration has small enough pore size such that endotoxins won't go through 

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