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Microbiology Test 2
Chapter 7 - DNA to Protein
14
Biology
Graduate
11/05/2008

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Term
  1. genome
  2. gene
  3. genetics
  4. genomics
Definition
  1. complete set of genetic info
  2. functional unit of genome
  3. study of transfer of genes
  4. study of sequence of DNA
Term
Living cells must accomplish two general tasks to multiply:
Definition
  1. DNA replication
  2. DNA expression (transcription - DNA to RNA, translation - RNA to protein)
Term
What makes up DNA?
Definition
  • phosphate group
  • 5-carbon sugar (deoxyribose)
  • nitrogenous base
Term
Nitrogenous Bases
Definition
  1. Adenine (A) - purine
  2. Guanine (G) - purine
  3. Thymine (T) - pyrimidine
  4. Cytosine (C) - pyrimidine
Term
How are DNA joined together?
Definition
  • they differ at the ends (5' and 3')
  • phosphate group attaches to the #5 carbon and the hydroxyl (OH-) group attaches to the #3 carbon
  • double stranded... A with T, C with G with hydrogen bonds
  • 2 DNA strands attach in opposite directions
Term
RNA
Definition
  • made up of ribose and nitrogenous bases (A, G, C, U - Uracil instead of Thymine)
  • single-stranded
  • RNA molecule called transcript
  • 3 different functional groups - messenger RNA (mRNA), ribosomal RNA (rRNA), transfer RNA (tRNA)
Term
DNA Replication
Definition
  1. DNA replicates to make second copy (identical)
  2. replication is bidirectional
  3. the 2 strands are unwound and separated; free, unbound nucleotides match up to newly separated bases of parent (template) strand
  4. each new strand of DNA consists of one parent strand and one daughter strand
Term
  1. primer
  2. DNA gyrase
  3. DNA ligase
Definition
  1. fragment of DNA to which DNA polymerase can add nucleotides
  2. enzyme that temporarily breaks the strands of DNA
  3. enzyme that seals gaps b/w 2 DNA fragments by forming covalent bonds
Term
  1. DNA polymerase
  2. helicases
  3. primase
Definition
  1. enzyme that synthesizes the DNA; uses one strand of DNA as template; occurs 5'--->3'
  2. enzymes that breaks down hydrogen bonds between the 2 strands- bonds b/w A-T and G-C
  3. enzyme that synthesizes small fragments of RNA
Term
  1. Okasaki fragment
  2. Origin of Replication
Definition
  1. nucleic acid fragment generated during discontinuous replication of the lagging strand of DNA
  2. region of DNA where where replication begins
Term
Transcription
Definition
  • synthesis of a strand of mRNA from a DNA template
  • mRNA carries coded info from DNA to ribosome

 

Term
Translation
Definition
  • decoding info held in the mRNA to synthesize proteins
  • rRNA builds ribosomes
  • tRNA recognizes sequences of mRNA and transports the required amino acids to form polypeptide chain
Term
Codons
Definition
  • group of 3 nucleotides
  • 64 codons (61 are sense codons, 3 are nonsense [stop] codons)
  • start codon = AUG (methionine); stop codons = UAA, UAG, UGA
Term
More Translation
Definition
  • in response to each codon, tRNA brings its anticodon (ribosomes direct the binding)
  • tRNA binds to P site then A site
  • P site and A site attach through peptide bond then detach from tRNA
  • translation terminates at stop codon
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