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Microbiology terms
terms from micro at QUT brisbane
50
Biology
Professional
10/29/2008

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Term
what is the size of a virus ?
Definition
18 nanometers - 2.2 micro meters
Term
what are the 3 basic morphological virus types?
Definition
Helical, Isohedral, complex
Term
what is a bacteriophage?
Definition
virus that infects bacteria
Term
plant virus's have mostly what type of nucleic acid?
Definition
RNA
Term
Do virus's replicate or reproduce?
Definition
replicate
Term
What is the cytopathic affect?
Definition
Cytopathic effect (CPE) refers to degenerative changes in cells (especially in tissue culture) associated with the multiplication of certain viruses.
Term
what are 3 visible changes to cells on a monolayer when innoculated with a virus?
Definition
Cell rounding and gaps, synctia (multinucleated giant cells), inclusions
Term
what are 5 ways of controlling human virus Disease?
Definition
1.hygiene, 2. vector, 3.lifestyle changes, 4.antiviral agents, 5 vaccinaton
Term
what are 5 ways anti-viral agents work?
Definition
1. interfere with virus attachment/entry
2. interfere with virus fusion
3. inhibit virus transcription
4. Inhibit virus assembly
5. interefer with virus release
Term
what is Acyclovir used for?
Definition
herpesvirus (it inhibit virus transcription/replication)
Term
what are the 3 types of vaccines?
Definition
live (attenuated), killed (inactivated), sub-unit (genetically engineered)
Term
Prions cause what?
Definition
transmissible spongiform encephalopathies
Term
what are some human TSE's?
Definition
Creutzfedt-Jakob disease
Term
mad cow disease is caused by a what?
Definition
prion
Term
what is the normal healthy gene that codes for protein
Definition
PrPc which is found mainly on nuerons and thought to be involved in synpatic function
Term
Bacteria are of which kingdom
Definition
monera
Term
what is size of bacteria cells
Definition
1-6 micro meters
Term
Bacteria are all chemoheterotrophic, what is this?
Definition
feeding on organic material without photosynthesis: used to describe an organism that obtains its nourishment from organic, as opposed to inorganic, substances and does not require light energy for its metabolism.
Term
the colour of basic dyes is in what ion (pos or neg)
Definition
postivie ion
Term
the colour of acidic dyes are in the what ion?
Definition
negative ion
Term
Bacterial cells are slighlty negative or positive charged?
Definition
negative charged
Term
safranin, and crystol violet will be basic or acidically charged
Definition
basic (positively) charged
Term
Negative staining stains the inner parts of the cell true or false..
Definition
F, it does noit stain the cell but stains the background
Term
when desribing bacteria under a microscope, which thing will u mention
Definition
shape, arrangement, size, inclusions (spores,cysts)
Term
a colony has how many types of bacteria?
Definition
1
Term
growth on an agar, that is not required or expected is called a what?
Definition
contaminant
Term
what is a halophile
Definition
salt loving bacteria
Term
what is a microaerophile
Definition
a bacteria that needs very small mount of O2
Term
A mesophile loves what?
Definition
18-20 degrees
Term
The macConkey Agar contains what?
Definition
bile salts (git bacteria), dyes (select G-ve), and salt (halophiles)
Term
what is indole test used for?
Definition
E Coli is th main positive
Term
what is the urease test used for?
Definition
Proteus is the main positive..
Term
what is oxidase used for?
Definition
Divides G-ve bacilli into 2 grps
Term
what is catalase used for?
Definition
divides G+ve cocci into 2 grps
Term
pseudomonadaceae are all oxidase what/
Definition
positive
Term
enterobacteriacease are all oxidase what
Definition
negative
Term
what is seen with the indole test?
Definition
if positive them powder tuns blue (e coli)
Term
importantly gram positive cocci are very similar in microscopic appearance... so how would you tell staph from strept?
Definition
strep are catalse negative, while staph is catalase positive
Term
what is added in the catalase test?
Definition
H202 hyrdrogen peroxide
Term
define infection...
Definition
presence or multiplication of pathogenic microbes in or on the body
Term
what is a disease/
Definition
harmful alteration of physiological state of host
Term
define a vehicle in terms of infection
Definition
an inanimate substanece within or upon which infectious agents may be delivered to a suspectible host
Term
malaris is carried by what?
Definition
anopheles mosquito
Term
sterility
Definition
complete absence of anty type of living organism
Term
sterlisation
Definition
killing all organisms
Term
disinfection
Definition
killing most organism to an acceptable level
Term
define thermal death point..
Definition
lowest temp where all microbes will be killed in a liquid suspension in 10 minutes
Term
how hot is the autclave for material to be sterile in 15 mins
Definition
121 degrees
Term
at 134 degrees how long must the material remain in autoclave to be sterile
Definition
134 mins
Term
what heat is required for dry heat, for how long
Definition
180 for 60 mins
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