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Microbiology Lecture 6
Transporters
21
Microbiology
Undergraduate 2
09/04/2011

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Term

Passive Transport 

Definition

transport of a substance across a cell membrane by diffusion, uses gradient energy 

transports with gradient(cannot use to concentrate nutrients)

Term
Active Transport 
Definition

The movement of ions or molecules across a cell membrane into a region of higher concentration, assisted by enzymes and requiring energy

uses PMF or ATP for energy 

Term
Primary Active Transport 
Definition
 also called direct active transport, directly uses energy to transport molecules across a membrane
Term
ABC ATPase 
Definition

transporters use ATP in addition to a signal from a periplasmic binding protein to open a transport channel and import a solute. 

 

provides a cap that prevents backflow

Term

 

Secondary active transport

Definition
In secondary active transport, in contrast to primary active transport, there is no direct coupling of ATP; instead, the electrochemical potential difference created by pumping ions out of the cell is used
Term
MFS transporters
Definition

are single-polypeptide secondary carriers capable only of transporting small solutes in response to chemiosmotic ion gradients

 

 

proteins use energy from gradient of one ion (H+) to move another ion or uncharged solute against its gradient
Term

 

uniport

Definition

 

uses electrical potential (negative inside) like a magnet to pull (+) charged ions into the cell or (-) out
 
A channel protein that transfers only one substrate at a time across the.
Term

symport

Definition
 a mechanism of transporting two compounds simultaneously across a cell membrane in the same direction, one compound being transported down a concentration gradient, the other against a gradient
Term

 

antiport

Definition
  • An antiporter (also called exchanger or counter-transporter) is an integral membrane protein which is involved in secondary active transport of two or more different molecules or ions (i.e. solutes) across a phospholipid membrane such as the plasma membrane in opposite directions.
Term

 

rocker-switch mechanism

Definition

how gated channels open and close

when something moves across the membrane a gate closes behind it to ensure it will not escape.

Term

 

Phosphotransferase system

Definition

is a distinct method used by bacteria for sugar uptake where the source of energy is from phosphoenolpyruvate (PEP)

 

 

uses a phosphate “relay” to transport glucose
Term

 

Type I secretory system

Definition

 

can cross both membranes in one step
Term

 

Drug efflux pump

Definition
Example of Type I transporter that uses the PMF rather than ATP to power transport
Term

 

Type III secretory system

Definition

 

can cross both membranes in one step
 
 
protein is only secreted once bacterium has contacted host cell
Term

 

needle complex

Definition

part in type III secretory system

 

Transport is blocked until the needle complex contacts a target cell.

Term

 

syringe secretion

Definition

in type III secretory systems

 

protein is secreted DIRECTLY into host (like a syringe)
Term
Type II secretory system
Definition

can have a periplasmic intermediate

 

requires a signal sequence

 

depend on the Sec or Tat system for initial transport into the periplasm. Once there, they pass through the outer membrane via a multimeric (12-14 subunits) complex of pore forming secretin proteins

Term
Chaperone
Definition
part of Type II secretory system
Term
signal sequence
Definition
part of type II secretory system
Term
sec translocon
Definition
part of type II secretory system
Term
TAT secretory system
Definition
can cross the inner membrane fully folded
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