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Microbiology Lecture 5 Vocab
Structures
35
Biology
Undergraduate 2
01/24/2009

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Term
Semipermeable Membrane
Definition

Membrane that allows the passage of some but not all molecules

 

Phospholipid Bilayer that allows water and small nonpolar solutes to pass, but not polar molecules or large molecules.

Term
Aquaporin
Definition
Open channels for water in membrane that allows free passage of water
Term
Gated Channel
Definition
Gates on channels in the membrane that do NOT allow free passage of polar molecules and ions
Term
Transporter
Definition

Type of protein found in cell membranes that functions in the transport of certain compounds (large molecules) across the membrane; may be called a permease or a carrier. Only OUT!

 

Need energy

 

Transport in: mostly monomers, big things need to be broken down

 

Transport out: big proteins can leave b/c of special transport

Term
Osmosis
Definition
Movement of water across a membrane from a dilute solution to a more concentrated solution because the solute cannot move across the membrane
Term
Hyperosmotic
Definition

A solution that is more concentrated than the fluid inside the cell.

 

Water leaves the cell causing the cell to shrivel

Term
Hypoosmotic
Definition

Solution outside of a cell that is less concentrated than the cell.

 

Water will move into the cell and cause the cell to swell or potentially lyse if no cell wall present

Term
Isoosmotic
Definition

Where the concentration in the solution and inside the cell are the same

 

No net movement of water so the cell remains the same size

Term
Lysis
Definition
When the cell bursts
Term
Turgor Pressure
Definition
Main pressure of the cell contents against the cell wall in plant cells and bacteria cells due to osmosis
Term
Plasmolysis
Definition
Process in which water diffuses out of a cell, causing the cytoplasm to dehydrate and shrink from the cell wall
Term
Osmoprotection/Osmoprotectant
Definition
Solutes that the cell produces when in a hypertonic solution so that less water leaves the cell
Term
Proton Motive Force
Definition

Form of energy generated by the electron transport chain, which expels protons to create a chemiosmotic gradient

 

When the electron transport chain ejects protons from the cell creating an electrochemical gradient which is a form of energy

 

The controlled flow of protons back into the cell release energy

 

Δp = ΔΨ + ΔpH

Term
Membrane Potential (ΔΨ)
Definition

Separation of charge across the membrane (positive outside and negative inside)

 

Protons want to go inside cell, but can't because they are charged

Term
Proton Gradient (ΔpH)
Definition
More protons outside the cell than inside so protons want to come in to equalize the concentration on both sides.
Term
Proton Pump
Definition

Complex of electron carriers in the electron transport chain that ejects protons from the cell

 

Used to create the proton gradient and proton motive force

Term
Respiratory Chain
Definition

Electron pairs go through a series of redox reactions and the ΔGrnx is used for energy to pump protons out of the cell

 

Electron donor: NADH

Electron acceptor: O2 

Term

Bacteriorhodopsin

Definition
Uses light energy to make proton gradient
Term
Peptidoglycan
Definition

Material that makes up the cell wall for bacteria

 

Important for cell shape and to hold in turgor pressure

Term
N-acetyl glucosamine (NAG)
Definition

One of the sugars that is the backbone of peptidoglycan

 

Links with NAM with β(1-4) bonds

Term
N-acetyl muramic acid
Definition

One of the sugars that is the backbone of peptidoglycan

 

Links with NAG with β(1-4) bonds
Term
Peptide Side Chain
Definition

Peptides that come off of NAM to help crosslink

 

4 R-groups

Term
Peptide Crosslinking
Definition

Connect the peptide chains that come off of NAM

 

Pentaglycine bridge

 

See figure in note for full picture

Term
Transpeptidation
Definition

AA side chains make peptide bonds

 

Cross link side chain

 

 

Term
Transglycosylation
Definition

Hook sugars together

 

Need enzyme to break chain and need another enzyme to put in monomer

Term

pentapeptide cross bridge (= pentaglycine bridge)

Definition

Gram (+) Bacteria

 

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Term
teichoic acid / lipoteichoic acid
Definition

On Gram (+) Bacteria

 

They are antigens

 

They trigger the body to produce antibodies against the bacteria

Term
Lipoprotein
Definition

Gram (-) bacteria

 

Macromolecule formed by the bonding of lipid to protein

 

Anchors outer membrane to PG layer

 

Made up of three porins

 

See figure in notes

 

 

Term
Porin
Definition

Gram (-) Bacteria

 

Proteins in the outer membrane of Gram-negative bacteria that form channels through which small molecules can pass

Term
Outer Membrane
Definition
Lipid bilayer that surrounds the PG layer of Gram negative bacteria
Term
Lipopolysaccharide (LPS)
Definition

Part of outer membrane of gram (-) bacteria

 

Molecule formed by bonding of lipid to polysaccharide

 

Lipid A + Core Polysaccharide + O-antigen

 

Endotoxin (toxic to our body)

Term
Core Polysaccharide
Definition

In gram negative bacteria

 

A part of LPS

Term
O-antigen
Definition

Gram (-) Bacteria

 

Part of LPS

 

Antigenic polysaccharide portion of lipopolysaccharide (LPS), the molecule that makes up the outer leaflet of the outer membrane of Gram negative bacteria

 

See figure

Term
Mycolic acids
Definition

Make up mycobacterium cell wall around PG

 

Anchored to sugars to long fatty acids

 

Make up the waxy layer around some bacteria which makes it hard to stain and kill them

Term
Mycoplasma
Definition

Has no cell wall

 

Can take any shape

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