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| Hydrolyzes (Cuts) DNA @ particular sequence |
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| The sequence where DNA is hydrolyzed (cut) by restriction endonucleases |
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Enzyme that puts the cut ends of DNA back together through dehydration between 5' P and 3' OH (paste) |
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| The palodromic sequences that have been cleaved and then the ends are brought back together by complementary sequences |
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| When the complementary ends of two different DNA strands are combined |
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The process of separating pieces of DNA (or anything else) based on size (DNA is negatively charged so it moves toward the positive electrode) Smaller piece, larger movement and vise versa |
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Recognize a specific DNA sequence where ever it is Is labeled with light, color, reactivity to recognize where it binds |
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Probe has a free 3' OH which can be used as primers to begin DNA replication (sequencing) where ever you want process of joining two complementary strands of DNA |
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Probe has a free 3' OH which can be used as primers to begin DNA replication (sequencing) where ever you want which can be used to create a specific mutation Can synthesize DNA with a particular mutation |
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| A collection of one organism's DNA fragments that are stored within a host organism |
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| The fragments that are generated with restriction enzymes after DNA from an organism is isolated for its DNA library |
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| A small piece of DNA into which a foreign DNA fragment can be inserted |
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| The cell that gets the cloning vectors inserted into them |
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| Colony hybridization/ Library Screening |
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| Paper picks up each bacterial colony in the same way it was on the agar plate. The probe then binds to the DNA sequence on the filter paper to identify it |
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Add a gene with an easily detectable product into the middle of another gene When the promoter is "ON" the detectable product is made (reports the activity of the promoter) Allow easy detection of transcription from a promoter |
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| Green Fluorescent Protein (GFP) |
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Definition
Inserted into a gene to report the activity of the promoter When the gene is on the cells are green |
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Isolating a DNA sequence and figuring out where it is located Uses probes to find DNA fragments in an electrophoretic gel |
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| Polymerase Chain Reaction (PCR) |
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Amplifying a particular DNA sequence (1 copy of DNA to trillions) Three Steps: 1. Denature the DNA (heat) 2. Bind specific primers to DNA 3. Make new DNA from primers |
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| PCR Screening (for diseases) |
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By specifically amplifying the gene If the gene isn't present then there is no amplification |
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| Similar to making a library, except only a specific gene is inserted into the new host |
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| transgenic / genetically-modified organism |
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| An organism that possess a gene or genes that have been transferred from a different species |
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Allows you to compare the amount of mRNA produced from each gene under different conditions More red mRNA produced it shows up red More green mRNA produced it shows up green Equal amounts of green and red mRNA shows up yellow |
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Complementary DNA doublestranded DNA version of an mRNA molecule |
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