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Microbiology Lab
Exam 3
29
Biology
Undergraduate 4
11/16/2008

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Term
Standard Plate Count of Milk
Definition
most reliable to determine sanitary quality of milk, High numbers means there is a higher liklihood of disease transmission, but does not means pathogens are present
Term
Quality Control of Milk--Total Coliform Test
Definition
enumeration of coliforms;
uses violet Red Bile Agar
Term
Violet Red Bile Agar
Definition
selective -- gram-negatives;
differential -- lactose fermentation
positive: purple/red with red haze of bile precipitate
Term
Ouchterlony
Definition
antigen-antibodies complexes form and precipitate.
identity: curved line;
non-identity: crossed lines;
partial-identity: curved line with spur
Term
What organism causes Strep throat, and what does the Strep test detect on the organism?
Definition
Strep. pyogenes;
Lancefield Group A antigen
Term
Direct Agglutination
Definition
detcts O and H antigens of enteric bacilli;
positive: clumping, for Shigella or Salmonella antiserum
Term
Rapid Staph ID test
Definition
positive for S. aureus: grey clumps
Term
ELISA
Definition
Enzyme Linked ImmunoSorbent Assay;
for the detection of antigens in bodily fluids.
Term
Bacteriophage
Definition
a virus that attacks bacteria through the lytic cycle
Term
plaque
Definition
the virus will kill cells that will create a clearing zone in the bacterial growth
Term
Ammonification
Definition
amine groups are removed from amino acids and converted into ammonia; production of ammonia from organia ammonia.
Term
Nitrification
Definition
oxidation of nitrogen in the ammonium ion to produce nitrite; nitrite is then oxidied into nitrate.
Term
Nitrogen Fixation
Definition
the conversion of atmospheric nitrogen to ammonia
Term
Denitrification
Definition
the conversion of nitrates to free nitrogen
Term
Coliforms
Definition
small, non-sporulating, Gram-negative facultative rods that ferments lactose to acid and gas
Term
Endo Agar
Definition
selective: gram- (-); diff.:lactose ferm.; positve: green metallic sheen
Term
KF strep Agar
Definition
sele: gram- (+); diff: maltose and latose ferm.; positive: yellow/red/brown colonies.
Term
FC Agar
Definition
Sele: gram (-); Diffe: gram(-); positive: blue colonies.
Term
what is used to test for ammonification, and what happens?
Definition
Nessler's reagent; yellow or brown color change.
Term
What is used to test for nitrate production, and what happens?
Definition
Trommsdorf's reagent, Sulfuric acid, Diphenylamine reagent; blue-black color change.
Term
what are some common pathogenic microbes found in food?
Definition
Salmonella, Shigella, E.coli, Listeria monocytogenes, Staph. aureus
Term
Staphylococci
Definition
gram (+), fac anaerobe, clusters, skin normal flora
Term
beta-Lactamase Test
Definition
a rapid test to determine is a bacterium is resistant to penicillins ot cephalasporins
Term
Steptococci
Definition
gram (+), catalase negative, chains, skin normal flora, divided into group A,B,C,D, Pneumococcus, or viridans
Term
Enterotube II
Definition
to identify gram-negative enteric bacilli based on biochemical testing; 14 test in one tube; 24 hr. incubation and obtain code from Enterotube Code Book.
Term
Epidemiology
Definition
the study of the causes, occurrence, transmission, distrubution, and prevention of disease
Term
Index case
Definition
the first person that is ingected with a disease
Term
prevalence rate
Definition
the total number of cases of a disease occuring in a population during a particular time period
Term
prevalence rate
Definition
total # of cases/# of people in Pop.
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