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microbiology final hornstein
final Exam 2006
156
Biology
Undergraduate 2
12/16/2006

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Term
The edema from hay fever occurs when a basophil...
Definition
Releases histamine
Term
The transfer of DNA from one bacteria to another using a PHAGE is...
Definition
transduction
Term
An organism which produces inorganic metabolic end products from glucose without using oxygen is
Definition
using anaerobic respiration
Term
Complement fixation results in
Definition
Cell Lysis
Term
interferon is part of the bodies nonspecific or specific defenses
Definition
non specific
Term
when an antibiotic quits working for you it means that
Definition
selection pressures have left only the resistant bacteria
Term
If I mix dead bacteria with live ones, DNA may transfer, this is an example of
Definition
transformation
Term
If I am born lacking the gene to make IL-1, My macrophages cannot stimulate my
Definition
T- Helper cells
Term
what are the three things that contribute to virulence of a microbe
Definition
Cell wall structures, Toxins, and Enzymes
Term
If your surface markers are such that a virus can not attach to them then you have _______ to that virus
Definition
Innate Immunity
Term
Antibiotics which kill only a selective group of bacteria are called what
Definition
Narrow Spectrum
Term
If you have antibodies to an organism it indicates
Definition
you have at some point in the past been exposed to that bug
Term
Paper towels, combs and silverware are all examples of potential
Definition
Fomites
Term
The killing of all vegetative bacteria and spores is
Definition
Sterilization
Term
The introduction of a new disease causing bacteria into a population tends to be devastating because...
Definition
the population has no immunity
Term
Which chemical would be more effective
A.Liminol- Coefficient 0.2
B.Ammonium Chlorate- P.E 0.4
C.Quaternary Ammonium Sulfate- P.E 1.2
Definition
the higher the P.E. the better...
C)
Term
What are the predisposing factors for infection
Definition
Age, Diet, and Immunosuppression
Term
Filtration has limited effectiveness in controlling microbes and their diseases because
Definition
toxins are not removes, viruses may pass through, and mycoplasmas may sneak through
Term
Antigen
Definition
What the body views as "foreign"
Term
Bacteriostatic
Definition
Any process or agent that inhibits bacterial growth
Term
Interferon
Definition
Most effective chemical defense your body has against infection
Term
Portal of Entry
Definition
Examples include, Gastrointestinal tract, and skin, mucous membranes
Term
T-Killer cells
Definition
Lymphocyte which targets virally-infected cells
Term
Fever
Definition
Nonspecific defense
Term
Antibody
Definition
Protein secreted by plasma cells
Term
Zoonoses
Definition
A microbe which jumps to humans from another species.
Term
DNA sequesnces which code for capsules, pigments, and antibiotic resistance are found on
Definition
Plasmids
Term
High levels of IgE and Eosinophils indicate
Definition
Worm infections and Allergies
Term
Scrubbing well with soap is an example of
Definition
Sanitation
Term
The gene for Antibiotic resistance is most likely found on
Definition
a Plasmid
Term
The cell which is attacked during an immune response is the
Definition
Antigen
Term
A person who can contaminate others with a pathogen without becoming sick themselves is
Definition
A carrier
Term
Salts control bacteria by
Definition
Dehydrating them
Term
The cell which notifies the T-helper that it is time to attack the antigen
Definition
Macrophage
Term
Some bacteria have the ability to resist antibiotics, while others of the same species can not this variation is due to
Definition
Different alleles
Term
when evaluating a new Antimicrobial drug you need to take into consideration
Definition
the type of microbe you are trying to control, can you get effective levels to the site of infection, is the drug toxic to the patient, does the microbe develop resistance readily, and other drugs by which the chemical may react
Term
what is the application of heat that selectively kills spoilage organisms without greatly altering the quality of the food
Definition
Pasteurization
Term
The ability of a pathogen to infect an organism is regulated by
Definition
the host species, the portal of entry, and the immune status of the potential host
Term
Attenuated Vaccine
Definition
Vaccine consisting of "Tamed" microbes; may revert to a wild, infection form
Term
Attenuated Vaccine
Definition
Vaccine consisting of "Tamed" microbes; may revert to a wild, infection form
Term
Plasma Cell
Definition
Antibody producing cell
Term
Monocyte
Definition
Phagocytic, agranular leukocyte which initiates the antibody response
Term
PMN
Definition
Most numerous Phagocytic leukocyte; granular
Term
Edema from an allergic reaction is due to histamine released from these leukocytes
Definition
Basophil
Term
Killed Vaccine
Definition
Vaccine typoe which can provide DNA for transformation. Often requires a large dose and more boosters to be effective. Ex. Cholera. The diseases are treated with formalin, radiation, heat or some other agent that does not destroy antigenicity. Surface markers intact. Typhoid Vaccine
Term
the skin and mucous membranes are part of the bodies
Definition
non specific defenses
Term
Type 1 hypersensitivity is due to reaction s of the
Definition
Ige and Mast Cells
Term
Streptomyces is a common soil oragnism it is also
Definition
Normal flora
Term
The primary virulence factor for streptococcus mutans (teeth bacteria) is
Definition
Lipopolysaccharide- (only in bacteria)
Term
IL-1 is secreted by the___ to activate the____
Definition
Antigens, B-Cells
Term
The only antibody to provide transplacental protection is the
Definition
Ig G
Term
Type III hypersensitivities are due to innapropriate responses by
Definition
IgM, Ig G, and Complement Proteins
Term
Loss of Sensation in fingers and toes, numerous small cuts, oozy brown lesion. When you streak it out you find an acid fast rod. Streak plates grew nothing. Most probable causative agent it
Definition
Mycobacterium Leprae
Term
Diapedesis
Definition
The ability of the WBC's to leave the bloodstream and move to the site of infection in the tissues
Term
Immunological memory (anamnestic response) prevents you from becoming sick with the same bug because...
Definition
the time from contact to antibody response is dramatically shortened
Term
After eating Salmon, you develop nausea, blurred vision, and you have a hard time getting your fingers to hold the phone to call the doctor. The salmon is cultured and an anaerobic spore forming rod is found. This is probably
Definition
Clostridium Botulinum (From food poisoning)
Term
Gr+ bacteria rarley infect the eyes because
Definition
Lysozyme in tears ruptures the cell wall
Term
Antibody Production is limited by the
Definition
T-Suppressor
Term
One of the Antigenic determants you find on the surface of a memory cell is
Definition
Ig G
Term
Virulence Factors
Definition
Capsules, Neurotoxins, and Hemolysins
Term
Micrococcus Luteus
Definition
Gr+ Cocci which is normal skin flora, grows in bright yellow colonies, and is responsible for the unpleasant odor of old sweat socks.
Term
Hemophilus Influenzae
Definition
Gr- organism responsible for Menigococcal meningitis
Term
Coughing up blood, difficulty breathing, high fever, very pale skin, and thin. Chest X-ray shows calcified scar tissue bubbles in the lungs. This should be checked for
Definition
Mycobacterium Tuberculosis
Term
Live mutant which attacks the fimbriae, vaccine developed for Gonorrhea
Definition
Attenuated Vaccine
Term
Immunocomprimised, hacking cough, high fever, Sputum culture shows an assachrolytic, gr- diplococci, it is probably
Definition
Neisseria Meningitis
Term
Micrococcus Luteus
Definition
Normal Skin cocci which is a facultative anaerobe, produces ivory colonies, and is catalase positive
Term
Penicillin
Definition
A selective antibiotic used to treat children with
Term
Index case
Definition
The first person to cause the symptoms to come up with the illness
Term
You draw blood samples from all the patients and find no bacteria. this means
Definition
Nothing- Blood should be sterile
Term
The conversion of glucose to inorganic end products requires
Definition
Respiration and glycolysis
Term
If E.Coli is a facultative anaerobe which culture will produce the most lactic acid?
In glucose with O2
In Glucose without 02?
Definition
Anaerobic does not require 02 to grow so it therefore does not need oxygen
Term
If one recieves plasma what kind of immunization are they getting
Definition
Passive Artificial immunization
Term
Conjugation
Definition
The exchange of genetic material via pilli
Term
Wwhat is most effective at killing Candida?(people yeast)
Definition
A fungicide
Term
How can a microbe be highly pathogenic but have very low virulence
Definition
Pathogenicity refers to the ability of an organism to infect a new host. Many organisms are easily transmitted, but lack virulence factors- things like toxins- which make them lethal to a host
Term
what characteristics are unique about the bacterial genome
Definition
DNA in the plasmid
Term
What is a metabolic pathway
Definition
Seriies of chemical reactions catalyzed by enzymes
Term
Beneficial Effects of Microbes
Definition
Food, beer, wine, yogurt, milk cheese bread vinegar, pickles...
Term
-Static-
Definition
to stand still (stasis) to hold
Term
Transposons
Definition
Jumping Genes
Term
-Cide
Definition
To kill
Term
Characteristics of the ideal antimicrobial drug
Definition
highly toxic, cheap to make, low resistance, specific to bug
Term
What are the primary "attack points" for antibiotics
Definition
attack cell walls: peptidoglycan layer, 70s ribosomes
Term
Why wont antibiotics work on viruses?
Definition
They dont have peptidoglycan, dont have metabolism, no mitochondria. Basically all the targets are missing
Term
Normal Flora
Definition
Bacteria, viruses, fungus, bugs, that live in us and protect us. Ex. eyelashes, intestinal bugs provide nutrients
Term
Pathogenicity
Definition
The ability of an organism to enter a host and cause disease
Term
Virulence
Definition
Degree of damage by pathogen and how sick they make you. Toxins, Capsules, enzymes all work together against you
Term
Reservoir
Definition
place that will house the microbe and let it multiply
Term
Vector
Definition
Usually insects; transmits from one host to the other ex. malaria
Term
What is the difference between T-Cell and B-Cell immunity?
Definition
Lymphocytes start out in the bone marrow and either stay there and mature into B-Cells or they leave for the thymus gland, where they mature into T-Cells. B-cells and T-cells have different jobs to do. B-lymphocytes seek out their targets and send defenses to lock onto them. The T-Cells will then destroy the invaders identified by the B-Cells
Term
Humoral Immune System
Definition
Consists of B-Cells which originate in the bone marrow and stay there to mature. Memory against subsequent attacks
Term
Cell Mediated Immune system
Definition
Consists of T-Cells which originate in bone marrow but then go to the thymus to continue their development. These Destroy what the B-cells tell them to
Term
Complement
Definition
Fights viruses, cancer cells, and parasites
Term
NK (natural killer cell)
Definition
Activated by double strand RNA and fights off viral infections and tumor
Term
Eosinophils
Definition
Attack parasites and phagocyte antigen-antibody complexes
Term
Leukemia
Definition
Cancer of the bone marrow, typicall treated by killing the persons bone marrow, leaving them without any type of immune system. Must be extremely careful to avoid all possible pathogens.
Term
Antibodies-(immunoglobulin)
Definition
A protein produced by a B-lymphocyte that protects an organism against an antigen.
Term
IgG
Definition
Only Antibody to cross placenta. Long term immunity, Memory Antibodies
Term
IgA
Definition
Antibody on mucous membranes ex intestine, breast lung and genitourinary tract
Term
IgM
Definition
binds to antigens. many immune reactions
Term
IgD
Definition
B-cell activation, along with IgM
Term
IgE
Definition
Allergic reactions and worm infections Anaphylaxis, asthma...
Term
Sub Unit Vaccine
Definition
Can escape if mutated. Containing one or more pure or semi pure antigens
Term
Enterobacteraceae
Definition
Gr- Facultative rod, many animals and fish host the enteric form. Plague form in Rodents. Fleas act as a vector. Bubonic Plague transmittted by flea bite. Symptoms: Massive lymph nodes, high fever, extreme head and body aches, ecchymosis (bruising) Streptomycin, tetracycline, chloramphenicol.
Term
Clostridium Botulinum
Definition
Gr+ Rod, Spore former with very swollen central spores, strict anaerobe, will not tolerate acidic conditions, spores common in soil and water. Causes botulinism in food poisoning, Blurred vision, nausea, FLACCID PARALYSIS. Botox, Used to treat muscle spasms, cosmetic for wrinkles, migranes? Severe cross or wall eye
Term
In bacteria nonessential genetic material is stored in the
Definition
Ribosome
Term
A pure culture contains
Definition
Bacteria derived from a single "parent" bug
Term
Viruses have
Definition
DNA or RNA NEVER BOTH!!!
Term
Smallpox virus is
Definition
Latent
Term
If you want to find endospores, the best bacterial group to examine are the
Definition
BACILLUS
Term
An outer mycolic acid layer is characteristic of
Definition
Acid Fast Bacteria
Term
YEAST
Definition
A single celled fungus
Term
Aerotolerant Organisms obtain energy through
Definition
Fermentation
Term
An organism which uses carbohydrates as a carbon source is
Definition
An autotroph
Term
New organism, has a cell wall and is a photoautotroph, which kindom does it belong to?
Definition
Plantae
Term
Normal Flora are
Definition
Organisms that developed a symbiosis with us
Term
Patient has severe septicemia, (bllod infection) plating out blood samples shows nothing, but a chemical analysis of the blood shows peptidioglycan and Lipopolysaccaride. The patient has
Definition
Gr- Bacterial infection
Term
Psychrophile
Definition
An organism that would grow well in the refrigerator
Term
Organisms such as bacillus that convert nitrates to nitrogen are
Definition
Denitrifying bacteria
Term
Endospores are highly resistant to heat and drying because
Definition
they have almost no water
Term
Mesophils
Definition
Organisms likely to affect humans
Term
Crystal Violet
Definition
Primary Stain in a gram stain
Term
Ability of a virus to infect an organism is regulated by
Definition
the host species, the type of cells, and the availability of the attachment sites
Term
E.Coli, Micrococcus Luteus, and Lactobacillus all have what in common
Definition
They are normal flora for humans
Term
Which organism survives longer in a gram of sugar?
A)Strict Aerobe
B)Strict Anaerobe
Definition
Strtict anaerobe
Term
Solid media made from dandelion flowers and grass clipping extract
Definition
Non synthetic
Term
Vector
Definition
An insect that acts like a hypodermic needle, transferring infection from one host to another
Term
Archeobacterium
Definition
Bacterial group that lives in Extreme, usually lethal conditions but survives just fine
Term
Mycolic Acid
Definition
Chemical which makes some bacteria acid fast
Term
Lipopolysaccharide
Definition
Highly toxic layer found in some bacterial walls, ruptures red blood cells. Produces pathological effects such as fever
Term
Anabena
Definition
Bacteria that is used commercially
Term
An organism which gets its energy from sulfur and Iron
Definition
Heterotroph
Term
Mastigophora
Definition
single celled eukariotic organism which moves by pseudopods
Term
Purple Slide
Definition
Positive
Term
Red Slide
Definition
Red (wrong) negative
Term
Things moving on a slide would usually be
Definition
a protozoan
Term
A camper takes a drink from a lake, and soon develops intestinal distress he probably picked up
Definition
trophozoites- Common normal flora for deer, bear, moose, beaver..ect
Term
DNA Virus consists of
Definition
Adenosine, cytosine, uracil, and Guanine
Term
Why is it difficult to kill spores?
Definition
The spore coat resists heat, chemicals and drying
Term
Hyphae
Definition
Multicellular form of a virus
Term
Yeast
Definition
Single celled eukaryote
Term
Pili
Definition
Cytoplasmic extensions used to transfer genetic material between bacteria
Term
Capsule
Definition
Exterior coating which alters the pathogenicity of bacteria
Term
influenza virus
Definition
classic res infection, mutable virus with up to 8 strands of RNA, symptoms include, muscle ache, N, intestinal distress - are usually caused by secondary infections or you own immune sys
Term
Epstein Barr Virus
Definition
common DNA virus which can combine w/ environmental factors to produce cancers or mononucleosis; most people are carriers
Term
measles
Definition
infection characterized by fever, rash, KOPLIK spots, possible res or auditory complications, RNA virus
Term
Hepatitis A virus
Definition
transmitted by food or water, this RNA virus causes a self-limiting infection of the liver but produces no chronic carriers.
Term
hantavirus
Definition
"NEW" RNA virus , mice:vector, fluid in lungs
Term
polio virus
Definition
transmitted intestingally, headache, stiff neck, paralysis & extreme pain by attacking the CNS, RNA virus
Term
chicken pox virus
Definition
common childhood infection characterized by small skin lesions which may cause a latent infection of the peripheral nerves, member of the herpes family
Term
hepatitis B virus
Definition
trans by bld, causes liver infection associated w/ permanent liver damage and inc. risk of liver cancer; DNA virus
Term
All retroviruses use
Definition
revers transcriptase
Term
poison ivy is type I,II,III, or IV immune response
Definition
type IV hypersensitivity
Term
pneumocystis carnii pneumonia and cryptosporidium are common _________________ allowed to colonize due to HIV
Definition
secondary infections
Term
AIDS virus attacks the immune sys by destroying?
Definition
T-Helper Cells
Term
A microbe which moves readily from pet to human is a ______________
Definition
zoonoses
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