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Microbiology Exam 2 Microbial Systematics
N/A
31
Biology
Undergraduate 3
11/25/2012

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Term
Systematics
Definition
study of the diversity of organisms and their relationships
Term
What does systematics link?
Definition
taxonomy and phylogeny
Term
Taxonomy
Definition
the science of biological classification
Term
Identification
Definition
determining that a particular isolate belongs to a recognized taxon
Term
Classification
Definition
arrangement into groups
Term
Nomenclature
Definition
assignment of names to the taxonomic group
Term
What did taxonomy used to rely on?
Definition
phenotypic comparisons
Term
Now what approach is used in taxonomy?
Definition
Polyphasic
Term
What are the three parts of polyphasic
Definition
Phenotypic
Genotypic
Phylogenetic
Term
What are the five parts of phenotypic analysis?
Definition
Motility, Morphology, Metabolism, Physiology, and Cell Chemistry
Term
Morphology
Definition
colony morphology, Gram reaction, cell size and shape
Term
Motility
Definition
Nonmotile, gliding, swimming, swarming motility
Term
Metabolism
Definition
Utilization of C, N2 or S compounds, fermentation of sugars, N2 fixation
Term
Physiology
Definition
Temperature, pH and salt ranges for growth, response to O2, production of extracellular enzymes
Term
Cell chemistry
Definition
Fatty acids, polar lipids
Term
Fatty acid analysis
Definition
FAME fatty acid methyl ester
Term
What does FAME rely on?
Definition
variation in type and proportion of fatty acids present in membrane lipids
Term
Why does FAME require rigid standardization?
Definition
because FAME profiles can vary as a function of temperature, growth phase, and growth medium
Term
What does genotypic analysis provide?
Definition
Information at the DNA level
Term
What are the four types of genotypic analysis?
Definition
DNA-DNA hybridization
DNA profiling methods
Multilocus Profiling
GC ratios
Term
DNA-DNA hybridization
Definition
focuses on identifying species within a genus
Term
DNA profiling methods
Definition
Focuses on identifying strains within a species
Term
Multilocus profiling
Definition
Focuses on multiple genes
Term
GC ratios
Definition
Percentage of guanine plus cytosine bp in the genome
Term
Phylogenetic analysis
Definition
complation of both the phenotypic and genotypic info
Based on analysis of
Individual genes – 16S rRNA
Multiple genes
Whole genome
Term
What percent is the same species when comparing DNA-DNA hybridization
Definition
70%
Term
What percent is the same species in 16s RNA gene sequence identity
Definition
97%
Term
Bacteriological
Definition
The International Code of Nomenclature of Bacteria
Term
Taxonomic outline of the prokaryotes
Definition
Second edition of Bergey’s Manual of Systematic Bacteriology
Term
Bergey's manual
Definition
compendium of information on all recognized species of bacteria
Term
The prokaryotes
Definition
provides detailed information on the enrichment, isolation, and culture of the many groups of Bacteria and Archaea
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